Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2303 and GFF2304 are separated by 100 nucleotides GFF2304 and GFF2305 are separated by 106 nucleotides GFF2305 and GFF2306 are separated by 96 nucleotides
GFF2303 - Putative uncharacterized protein YiiQ, at 90,069 to 90,665
GFF2303
GFF2304 - Putative membrane protein, at 90,766 to 91,194
GFF2304
GFF2305 - Ferredoxin--NADP(+) reductase (EC 1.18.1.2), at 91,301 to 92,047
GFF2305
GFF2306 - Fructose-1,6-bisphosphatase, GlpX type (EC 3.1.3.11), at 92,144 to 93,154
GFF2306
Position (kb)
91
92
93 Strain fitness (log2 ratio)
-1
0
1 at 90.374 kb on + strand, within GFF2303 at 90.530 kb on + strand, within GFF2303 at 90.566 kb on - strand, within GFF2303 at 90.640 kb on - strand at 90.730 kb on + strand at 90.752 kb on + strand at 90.752 kb on + strand at 90.753 kb on - strand at 91.325 kb on - strand at 91.563 kb on + strand, within GFF2305 at 91.673 kb on + strand, within GFF2305 at 91.674 kb on - strand, within GFF2305 at 91.674 kb on - strand, within GFF2305 at 91.674 kb on - strand, within GFF2305 at 91.674 kb on - strand, within GFF2305 at 91.852 kb on - strand, within GFF2305 at 92.018 kb on - strand at 92.077 kb on + strand at 92.078 kb on - strand at 92.078 kb on - strand at 92.133 kb on + strand at 92.153 kb on - strand at 92.176 kb on + strand at 92.187 kb on + strand at 92.194 kb on + strand at 92.320 kb on + strand, within GFF2306 at 92.320 kb on + strand, within GFF2306 at 92.567 kb on - strand, within GFF2306 at 92.567 kb on - strand, within GFF2306 at 92.798 kb on - strand, within GFF2306 at 92.799 kb on + strand, within GFF2306 at 92.799 kb on + strand, within GFF2306 at 92.799 kb on + strand, within GFF2306 at 92.800 kb on - strand, within GFF2306 at 92.800 kb on - strand, within GFF2306 at 92.800 kb on - strand, within GFF2306 at 92.831 kb on - strand, within GFF2306 at 92.955 kb on + strand, within GFF2306 at 92.955 kb on + strand, within GFF2306 at 93.031 kb on + strand, within GFF2306 at 93.031 kb on + strand, within GFF2306 at 93.032 kb on - strand, within GFF2306
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 90,374 + GFF2303 0.51 -0.1 90,530 + GFF2303 0.77 -0.3 90,566 - GFF2303 0.83 -0.1 90,640 - -0.5 90,730 + -0.2 90,752 + -0.3 90,752 + -0.5 90,753 - -0.3 91,325 - -0.3 91,563 + GFF2305 0.35 -1.5 91,673 + GFF2305 0.50 -0.8 91,674 - GFF2305 0.50 -1.6 91,674 - GFF2305 0.50 +1.7 91,674 - GFF2305 0.50 -0.3 91,674 - GFF2305 0.50 -1.7 91,852 - GFF2305 0.74 +0.1 92,018 - +0.1 92,077 + +0.5 92,078 - -0.3 92,078 - +0.1 92,133 + +0.2 92,153 - +0.4 92,176 + -0.2 92,187 + +0.9 92,194 + -0.2 92,320 + GFF2306 0.17 +0.0 92,320 + GFF2306 0.17 +0.1 92,567 - GFF2306 0.42 -0.6 92,567 - GFF2306 0.42 +0.4 92,798 - GFF2306 0.65 -0.4 92,799 + GFF2306 0.65 -0.5 92,799 + GFF2306 0.65 -0.5 92,799 + GFF2306 0.65 -0.9 92,800 - GFF2306 0.65 +0.5 92,800 - GFF2306 0.65 +0.8 92,800 - GFF2306 0.65 -0.7 92,831 - GFF2306 0.68 -0.1 92,955 + GFF2306 0.80 +0.4 92,955 + GFF2306 0.80 -0.2 93,031 + GFF2306 0.88 +0.2 93,031 + GFF2306 0.88 +0.1 93,032 - GFF2306 0.88 +0.3
Or see this region's nucleotide sequence