Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2213

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2212 and GFF2213 are separated by 16 nucleotidesGFF2213 and GFF2214 are separated by 12 nucleotides GFF2212 - YihE protein, a ser/thr kinase implicated in LPS synthesis and Cpx signalling, at 2,120 to 3,106 GFF2212 GFF2213 - Periplasmic thiol:disulfide interchange protein DsbA, at 3,123 to 3,746 GFF2213 GFF2214 - 1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 3,759 to 4,622 GFF2214 Position (kb) 3 4Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 2.445 kb on + strand, within GFF2212at 2.502 kb on - strand, within GFF2212at 2.661 kb on + strand, within GFF2212at 2.661 kb on + strand, within GFF2212at 2.662 kb on - strand, within GFF2212at 2.945 kb on + strand, within GFF2212at 2.946 kb on - strand, within GFF2212at 3.152 kb on + strandat 3.167 kb on + strandat 3.206 kb on + strand, within GFF2213at 3.206 kb on + strand, within GFF2213at 3.264 kb on + strand, within GFF2213at 3.428 kb on - strand, within GFF2213at 3.435 kb on - strand, within GFF2213at 3.482 kb on - strand, within GFF2213at 3.482 kb on - strand, within GFF2213at 3.482 kb on - strand, within GFF2213at 3.541 kb on - strand, within GFF2213at 3.660 kb on - strand, within GFF2213at 3.798 kb on + strandat 3.828 kb on - strandat 3.830 kb on + strandat 3.951 kb on - strand, within GFF2214at 3.956 kb on - strand, within GFF2214at 3.993 kb on - strand, within GFF2214at 3.993 kb on - strand, within GFF2214at 4.032 kb on - strand, within GFF2214at 4.229 kb on - strand, within GFF2214at 4.260 kb on - strand, within GFF2214at 4.264 kb on - strand, within GFF2214at 4.326 kb on - strand, within GFF2214at 4.391 kb on + strand, within GFF2214at 4.392 kb on - strand, within GFF2214at 4.392 kb on - strand, within GFF2214at 4.401 kb on + strand, within GFF2214at 4.440 kb on + strand, within GFF2214at 4.441 kb on - strand, within GFF2214at 4.501 kb on + strand, within GFF2214at 4.501 kb on + strand, within GFF2214at 4.502 kb on - strand, within GFF2214at 4.502 kb on - strand, within GFF2214at 4.502 kb on - strand, within GFF2214at 4.542 kb on - strandat 4.670 kb on + strandat 4.744 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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2,445 + GFF2212 0.33 +0.0
2,502 - GFF2212 0.39 +0.4
2,661 + GFF2212 0.55 -1.8
2,661 + GFF2212 0.55 +0.5
2,662 - GFF2212 0.55 +1.2
2,945 + GFF2212 0.84 +0.0
2,946 - GFF2212 0.84 +0.7
3,152 + -2.6
3,167 + -4.7
3,206 + GFF2213 0.13 -1.8
3,206 + GFF2213 0.13 -2.7
3,264 + GFF2213 0.23 -3.6
3,428 - GFF2213 0.49 -3.3
3,435 - GFF2213 0.50 -3.8
3,482 - GFF2213 0.58 -1.5
3,482 - GFF2213 0.58 -2.0
3,482 - GFF2213 0.58 -1.5
3,541 - GFF2213 0.67 -2.9
3,660 - GFF2213 0.86 -2.8
3,798 + -0.0
3,828 - -0.2
3,830 + +0.6
3,951 - GFF2214 0.22 +1.0
3,956 - GFF2214 0.23 +0.6
3,993 - GFF2214 0.27 +0.3
3,993 - GFF2214 0.27 +0.5
4,032 - GFF2214 0.32 -0.2
4,229 - GFF2214 0.54 -0.5
4,260 - GFF2214 0.58 +0.2
4,264 - GFF2214 0.58 -0.9
4,326 - GFF2214 0.66 +0.4
4,391 + GFF2214 0.73 -0.0
4,392 - GFF2214 0.73 -1.2
4,392 - GFF2214 0.73 -0.3
4,401 + GFF2214 0.74 +1.1
4,440 + GFF2214 0.79 +0.6
4,441 - GFF2214 0.79 +0.1
4,501 + GFF2214 0.86 +0.3
4,501 + GFF2214 0.86 -0.7
4,502 - GFF2214 0.86 -0.3
4,502 - GFF2214 0.86 +0.1
4,502 - GFF2214 0.86 -0.3
4,542 - -0.5
4,670 + -1.1
4,744 + +0.5

Or see this region's nucleotide sequence