Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2189 and GFF2190 are separated by 185 nucleotides GFF2190 and GFF2191 are separated by 387 nucleotides
GFF2189 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 37,922 to 39,622
GFF2189
GFF2190 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 39,808 to 41,769
GFF2190
GFF2191 - Beta-lactamase class C and other penicillin binding proteins, at 42,157 to 43,455
GFF2191
Position (kb)
39
40
41
42 Strain fitness (log2 ratio)
-2
-1
0
1 at 38.871 kb on + strand, within GFF2189 at 38.871 kb on + strand, within GFF2189 at 38.871 kb on + strand, within GFF2189 at 38.872 kb on - strand, within GFF2189 at 39.109 kb on + strand, within GFF2189 at 39.136 kb on + strand, within GFF2189 at 39.137 kb on - strand, within GFF2189 at 39.141 kb on - strand, within GFF2189 at 39.240 kb on + strand, within GFF2189 at 39.250 kb on + strand, within GFF2189 at 39.251 kb on - strand, within GFF2189 at 39.354 kb on + strand, within GFF2189 at 39.557 kb on - strand at 39.571 kb on + strand at 39.572 kb on - strand at 39.573 kb on + strand at 39.702 kb on + strand at 39.702 kb on + strand at 39.703 kb on - strand at 39.703 kb on - strand at 39.753 kb on - strand at 39.819 kb on + strand at 39.832 kb on - strand at 39.934 kb on - strand at 40.006 kb on - strand, within GFF2190 at 40.032 kb on + strand, within GFF2190 at 40.032 kb on + strand, within GFF2190 at 40.035 kb on + strand, within GFF2190 at 40.122 kb on - strand, within GFF2190 at 40.674 kb on + strand, within GFF2190 at 40.675 kb on - strand, within GFF2190 at 40.713 kb on + strand, within GFF2190 at 40.723 kb on + strand, within GFF2190 at 40.724 kb on - strand, within GFF2190 at 40.725 kb on + strand, within GFF2190 at 40.725 kb on + strand, within GFF2190 at 40.775 kb on + strand, within GFF2190 at 41.044 kb on + strand, within GFF2190 at 41.045 kb on - strand, within GFF2190 at 41.122 kb on + strand, within GFF2190 at 41.123 kb on - strand, within GFF2190 at 41.258 kb on + strand, within GFF2190 at 41.302 kb on + strand, within GFF2190 at 41.382 kb on + strand, within GFF2190 at 41.412 kb on + strand, within GFF2190 at 41.492 kb on + strand, within GFF2190 at 41.565 kb on + strand, within GFF2190 at 41.565 kb on + strand, within GFF2190 at 41.602 kb on - strand at 41.614 kb on - strand at 41.768 kb on - strand at 41.768 kb on - strand at 42.025 kb on - strand at 42.144 kb on - strand at 42.173 kb on + strand at 42.173 kb on + strand at 42.197 kb on + strand at 42.215 kb on - strand at 42.298 kb on - strand, within GFF2191 at 42.350 kb on + strand, within GFF2191 at 42.627 kb on + strand, within GFF2191 at 42.627 kb on + strand, within GFF2191 at 42.628 kb on - strand, within GFF2191 at 42.739 kb on - strand, within GFF2191
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 38,871 + GFF2189 0.56 -0.8 38,871 + GFF2189 0.56 +0.0 38,871 + GFF2189 0.56 -0.1 38,872 - GFF2189 0.56 +0.9 39,109 + GFF2189 0.70 +0.3 39,136 + GFF2189 0.71 +0.3 39,137 - GFF2189 0.71 +0.1 39,141 - GFF2189 0.72 -0.8 39,240 + GFF2189 0.77 -0.0 39,250 + GFF2189 0.78 -0.7 39,251 - GFF2189 0.78 +0.3 39,354 + GFF2189 0.84 -0.1 39,557 - -0.1 39,571 + +0.2 39,572 - -1.8 39,573 + +0.7 39,702 + +0.6 39,702 + +0.3 39,703 - -0.3 39,703 - +0.4 39,753 - -0.9 39,819 + +0.7 39,832 - -0.1 39,934 - +0.0 40,006 - GFF2190 0.10 +0.2 40,032 + GFF2190 0.11 -0.3 40,032 + GFF2190 0.11 -0.8 40,035 + GFF2190 0.12 -0.1 40,122 - GFF2190 0.16 +0.2 40,674 + GFF2190 0.44 +0.6 40,675 - GFF2190 0.44 +0.2 40,713 + GFF2190 0.46 -0.4 40,723 + GFF2190 0.47 -0.3 40,724 - GFF2190 0.47 +0.6 40,725 + GFF2190 0.47 +0.3 40,725 + GFF2190 0.47 +0.2 40,775 + GFF2190 0.49 +0.1 41,044 + GFF2190 0.63 -0.1 41,045 - GFF2190 0.63 +0.3 41,122 + GFF2190 0.67 +0.2 41,123 - GFF2190 0.67 -0.3 41,258 + GFF2190 0.74 -0.1 41,302 + GFF2190 0.76 +0.7 41,382 + GFF2190 0.80 -0.0 41,412 + GFF2190 0.82 -0.2 41,492 + GFF2190 0.86 +0.9 41,565 + GFF2190 0.90 +0.3 41,565 + GFF2190 0.90 +0.2 41,602 - +0.7 41,614 - +0.0 41,768 - -0.2 41,768 - -0.1 42,025 - -0.0 42,144 - +0.1 42,173 + +0.0 42,173 + +0.0 42,197 + +0.6 42,215 - +0.1 42,298 - GFF2191 0.11 -0.3 42,350 + GFF2191 0.15 -0.5 42,627 + GFF2191 0.36 -1.6 42,627 + GFF2191 0.36 +0.0 42,628 - GFF2191 0.36 +1.5 42,739 - GFF2191 0.45 -0.4
Or see this region's nucleotide sequence