Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2188 and GFF2189 are separated by 184 nucleotides GFF2189 and GFF2190 are separated by 185 nucleotides
GFF2188 - RND efflux system, inner membrane transporter CmeB, at 34,624 to 37,737
GFF2188
GFF2189 - Nitrate/nitrite sensor protein (EC 2.7.3.-), at 37,922 to 39,622
GFF2189
GFF2190 - Glutamate synthase [NADPH] small chain (EC 1.4.1.13), at 39,808 to 41,769
GFF2190
Position (kb) 37
38
39
40 Strain fitness (log2 ratio) -2
-1
0
1 at 37.035 kb on + strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.194 kb on - strand, within GFF2188 at 37.205 kb on + strand, within GFF2188 at 37.294 kb on - strand, within GFF2188 at 37.508 kb on + strand at 37.509 kb on - strand at 37.509 kb on - strand at 37.605 kb on + strand at 37.610 kb on - strand at 37.634 kb on + strand at 37.763 kb on + strand at 37.764 kb on - strand at 37.833 kb on + strand at 37.838 kb on + strand at 37.923 kb on + strand at 37.966 kb on + strand at 38.042 kb on + strand at 38.043 kb on - strand at 38.108 kb on - strand, within GFF2189 at 38.488 kb on - strand, within GFF2189 at 38.496 kb on - strand, within GFF2189 at 38.589 kb on + strand, within GFF2189 at 38.644 kb on + strand, within GFF2189 at 38.871 kb on + strand, within GFF2189 at 38.871 kb on + strand, within GFF2189 at 38.871 kb on + strand, within GFF2189 at 38.872 kb on - strand, within GFF2189 at 39.109 kb on + strand, within GFF2189 at 39.136 kb on + strand, within GFF2189 at 39.137 kb on - strand, within GFF2189 at 39.141 kb on - strand, within GFF2189 at 39.240 kb on + strand, within GFF2189 at 39.250 kb on + strand, within GFF2189 at 39.251 kb on - strand, within GFF2189 at 39.354 kb on + strand, within GFF2189 at 39.557 kb on - strand at 39.571 kb on + strand at 39.572 kb on - strand at 39.573 kb on + strand at 39.702 kb on + strand at 39.702 kb on + strand at 39.703 kb on - strand at 39.703 kb on - strand at 39.753 kb on - strand at 39.819 kb on + strand at 39.832 kb on - strand at 39.934 kb on - strand at 40.006 kb on - strand, within GFF2190 at 40.032 kb on + strand, within GFF2190 at 40.032 kb on + strand, within GFF2190 at 40.035 kb on + strand, within GFF2190 at 40.122 kb on - strand, within GFF2190
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 37,035 + GFF2188 0.77 +0.3 37,194 - GFF2188 0.83 +0.2 37,194 - GFF2188 0.83 -0.1 37,194 - GFF2188 0.83 +0.7 37,194 - GFF2188 0.83 +0.4 37,205 + GFF2188 0.83 +0.7 37,294 - GFF2188 0.86 +0.3 37,508 + +0.3 37,509 - -0.0 37,509 - -0.6 37,605 + +0.0 37,610 - -0.7 37,634 + +0.2 37,763 + +0.2 37,764 - +0.4 37,833 + +0.0 37,838 + -0.2 37,923 + -0.2 37,966 + -0.1 38,042 + -0.0 38,043 - -0.1 38,108 - GFF2189 0.11 -1.3 38,488 - GFF2189 0.33 -0.4 38,496 - GFF2189 0.34 -0.9 38,589 + GFF2189 0.39 +0.9 38,644 + GFF2189 0.42 -1.2 38,871 + GFF2189 0.56 -0.8 38,871 + GFF2189 0.56 +0.0 38,871 + GFF2189 0.56 -0.1 38,872 - GFF2189 0.56 +0.9 39,109 + GFF2189 0.70 +0.3 39,136 + GFF2189 0.71 +0.3 39,137 - GFF2189 0.71 +0.1 39,141 - GFF2189 0.72 -0.8 39,240 + GFF2189 0.77 -0.0 39,250 + GFF2189 0.78 -0.7 39,251 - GFF2189 0.78 +0.3 39,354 + GFF2189 0.84 -0.1 39,557 - -0.1 39,571 + +0.2 39,572 - -1.8 39,573 + +0.7 39,702 + +0.6 39,702 + +0.3 39,703 - -0.3 39,703 - +0.4 39,753 - -0.9 39,819 + +0.7 39,832 - -0.1 39,934 - +0.0 40,006 - GFF2190 0.10 +0.2 40,032 + GFF2190 0.11 -0.3 40,032 + GFF2190 0.11 -0.8 40,035 + GFF2190 0.12 -0.1 40,122 - GFF2190 0.16 +0.2
Or see this region's nucleotide sequence