Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2152 and GFF2153 are separated by 32 nucleotides GFF2153 and GFF2154 overlap by 1 nucleotides GFF2154 and GFF2155 are separated by 155 nucleotides
GFF2152 - Putative membrane protein, at 41,855 to 43,123
GFF2152
GFF2153 - L(+)-tartrate dehydratase alpha subunit (EC 4.2.1.32), at 43,156 to 44,055
GFF2153
GFF2154 - L(+)-tartrate dehydratase beta subunit (EC 4.2.1.32), at 44,055 to 44,672
GFF2154
GFF2155 - Oxaloacetate decarboxylase gamma chain (EC 4.1.1.3), at 44,828 to 45,082
GFF2155
Position (kb) 44
45 Strain fitness (log2 ratio) -2
-1
0
1 at 43.302 kb on + strand, within GFF2153 at 43.306 kb on - strand, within GFF2153 at 43.460 kb on + strand, within GFF2153 at 43.460 kb on + strand, within GFF2153 at 43.487 kb on + strand, within GFF2153 at 43.488 kb on - strand, within GFF2153 at 43.585 kb on + strand, within GFF2153 at 43.585 kb on + strand, within GFF2153 at 43.587 kb on + strand, within GFF2153 at 43.591 kb on + strand, within GFF2153 at 43.592 kb on - strand, within GFF2153 at 43.592 kb on - strand, within GFF2153 at 43.594 kb on - strand, within GFF2153 at 43.594 kb on - strand, within GFF2153 at 43.696 kb on + strand, within GFF2153 at 43.697 kb on - strand, within GFF2153 at 43.987 kb on - strand at 43.996 kb on - strand at 44.028 kb on + strand at 44.141 kb on + strand, within GFF2154 at 44.161 kb on + strand, within GFF2154 at 44.565 kb on - strand, within GFF2154 at 44.656 kb on + strand at 44.705 kb on + strand at 44.724 kb on + strand at 44.770 kb on + strand at 44.786 kb on + strand at 44.786 kb on + strand at 44.786 kb on + strand at 44.823 kb on - strand at 45.073 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 43,302 + GFF2153 0.16 +0.1 43,306 - GFF2153 0.17 +0.4 43,460 + GFF2153 0.34 -0.3 43,460 + GFF2153 0.34 -1.0 43,487 + GFF2153 0.37 -0.2 43,488 - GFF2153 0.37 -0.1 43,585 + GFF2153 0.48 -0.1 43,585 + GFF2153 0.48 +0.2 43,587 + GFF2153 0.48 -0.1 43,591 + GFF2153 0.48 -0.0 43,592 - GFF2153 0.48 -0.6 43,592 - GFF2153 0.48 +0.1 43,594 - GFF2153 0.49 -0.4 43,594 - GFF2153 0.49 +1.0 43,696 + GFF2153 0.60 -0.5 43,697 - GFF2153 0.60 +0.4 43,987 - +1.0 43,996 - +0.5 44,028 + +0.3 44,141 + GFF2154 0.14 -1.8 44,161 + GFF2154 0.17 +0.3 44,565 - GFF2154 0.83 -1.3 44,656 + -0.2 44,705 + -0.4 44,724 + -0.2 44,770 + +0.0 44,786 + -1.6 44,786 + +0.4 44,786 + +0.1 44,823 - -0.4 45,073 + +0.0
Or see this region's nucleotide sequence