Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2081 and GFF2082 overlap by 4 nucleotides GFF2082 and GFF2083 overlap by 4 nucleotides GFF2083 and GFF2084 overlap by 23 nucleotides GFF2084 and GFF2085 are separated by 12 nucleotides
GFF2081 - Phosphate acetyltransferase (EC 2.3.1.8), ethanolamine utilization-specific, at 43,562 to 44,578
GFF2081
GFF2082 - ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17) @ ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17), ethanolamine utilization, at 44,575 to 45,378
GFF2082
GFF2083 - Ethanolamine utilization protein EutQ, at 45,375 to 46,064
GFF2083
GFF2084 - Ethanolamine utilization protein EutP, at 46,042 to 46,521
GFF2084
GFF2085 - Ethanolamine utilization polyhedral-body-like protein EutS, at 46,534 to 46,869
GFF2085
Position (kb) 45
46
47 Strain fitness (log2 ratio) -1
0
1 at 44.401 kb on + strand, within GFF2081 at 44.401 kb on + strand, within GFF2081 at 44.778 kb on + strand, within GFF2082 at 44.917 kb on + strand, within GFF2082 at 44.918 kb on - strand, within GFF2082 at 44.918 kb on - strand, within GFF2082 at 44.934 kb on + strand, within GFF2082 at 45.337 kb on - strand at 45.378 kb on + strand at 45.594 kb on - strand, within GFF2083 at 45.977 kb on + strand, within GFF2083 at 45.977 kb on + strand, within GFF2083 at 45.978 kb on - strand, within GFF2083 at 46.267 kb on + strand, within GFF2084 at 46.632 kb on - strand, within GFF2085 at 46.969 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 44,401 + GFF2081 0.82 -0.5 44,401 + GFF2081 0.82 +1.5 44,778 + GFF2082 0.25 +0.1 44,917 + GFF2082 0.43 +1.1 44,918 - GFF2082 0.43 +0.6 44,918 - GFF2082 0.43 +0.5 44,934 + GFF2082 0.45 +0.1 45,337 - +0.3 45,378 + +1.3 45,594 - GFF2083 0.32 -1.5 45,977 + GFF2083 0.87 +0.1 45,977 + GFF2083 0.87 -0.2 45,978 - GFF2083 0.87 -1.5 46,267 + GFF2084 0.47 +0.7 46,632 - GFF2085 0.29 +0.5 46,969 + -0.2
Or see this region's nucleotide sequence