Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2066 and GFF2067 are separated by 161 nucleotides GFF2067 and GFF2068 are separated by 128 nucleotides GFF2068 and GFF2069 are separated by 199 nucleotides
GFF2066 - Coproporphyrinogen III oxidase, aerobic (EC 1.3.3.3), at 29,224 to 30,123
GFF2066
GFF2067 - Putative membrane protein, at 30,285 to 30,473
GFF2067
GFF2068 - FIG01045300: hypothetical protein, at 30,602 to 31,879
GFF2068
GFF2069 - Ethanolamine operon regulatory protein, at 32,079 to 33,131
GFF2069
Position (kb) 30
31
32 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 29.623 kb on + strand, within GFF2066 at 29.624 kb on - strand, within GFF2066 at 29.848 kb on + strand, within GFF2066 at 30.100 kb on - strand at 30.132 kb on + strand at 30.132 kb on + strand at 30.137 kb on + strand at 30.169 kb on + strand at 30.245 kb on - strand at 30.358 kb on + strand, within GFF2067 at 30.369 kb on - strand, within GFF2067 at 30.415 kb on + strand, within GFF2067 at 30.551 kb on - strand at 30.561 kb on + strand at 30.566 kb on + strand at 30.685 kb on + strand at 30.686 kb on - strand at 30.728 kb on + strand at 30.729 kb on - strand at 30.773 kb on - strand, within GFF2068 at 30.869 kb on + strand, within GFF2068 at 30.911 kb on + strand, within GFF2068 at 31.021 kb on - strand, within GFF2068 at 31.370 kb on + strand, within GFF2068 at 31.504 kb on + strand, within GFF2068 at 31.733 kb on - strand, within GFF2068 at 31.777 kb on - strand at 31.782 kb on - strand at 31.831 kb on + strand at 32.133 kb on + strand at 32.133 kb on + strand at 32.226 kb on - strand, within GFF2069 at 32.259 kb on - strand, within GFF2069 at 32.328 kb on + strand, within GFF2069 at 32.328 kb on + strand, within GFF2069 at 32.329 kb on - strand, within GFF2069 at 32.346 kb on + strand, within GFF2069 at 32.347 kb on - strand, within GFF2069 at 32.414 kb on + strand, within GFF2069 at 32.447 kb on + strand, within GFF2069 at 32.494 kb on - strand, within GFF2069 at 32.494 kb on - strand, within GFF2069 at 32.514 kb on - strand, within GFF2069 at 32.514 kb on - strand, within GFF2069 at 32.639 kb on + strand, within GFF2069 at 32.640 kb on - strand, within GFF2069 at 32.640 kb on - strand, within GFF2069 at 32.688 kb on - strand, within GFF2069 at 32.722 kb on + strand, within GFF2069
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 29,623 + GFF2066 0.44 -0.1 29,624 - GFF2066 0.44 +0.2 29,848 + GFF2066 0.69 +0.2 30,100 - +0.1 30,132 + +1.8 30,132 + -0.4 30,137 + -1.6 30,169 + -0.0 30,245 - -0.9 30,358 + GFF2067 0.39 -0.1 30,369 - GFF2067 0.44 +0.3 30,415 + GFF2067 0.69 +0.0 30,551 - +0.3 30,561 + -0.7 30,566 + -0.4 30,685 + +0.2 30,686 - -0.2 30,728 + +0.4 30,729 - -2.2 30,773 - GFF2068 0.13 -0.7 30,869 + GFF2068 0.21 -0.4 30,911 + GFF2068 0.24 -0.3 31,021 - GFF2068 0.33 -0.0 31,370 + GFF2068 0.60 -2.6 31,504 + GFF2068 0.71 -1.3 31,733 - GFF2068 0.88 -0.2 31,777 - +0.1 31,782 - +0.6 31,831 + -0.9 32,133 + -0.0 32,133 + +2.1 32,226 - GFF2069 0.14 +0.9 32,259 - GFF2069 0.17 -0.2 32,328 + GFF2069 0.24 +0.4 32,328 + GFF2069 0.24 +0.0 32,329 - GFF2069 0.24 -0.1 32,346 + GFF2069 0.25 +0.2 32,347 - GFF2069 0.25 -0.9 32,414 + GFF2069 0.32 -0.1 32,447 + GFF2069 0.35 -0.1 32,494 - GFF2069 0.39 +2.9 32,494 - GFF2069 0.39 +0.2 32,514 - GFF2069 0.41 -0.1 32,514 - GFF2069 0.41 +0.8 32,639 + GFF2069 0.53 +0.3 32,640 - GFF2069 0.53 +0.1 32,640 - GFF2069 0.53 +0.6 32,688 - GFF2069 0.58 -0.3 32,722 + GFF2069 0.61 +0.1
Or see this region's nucleotide sequence