Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2044 and GFF2045 are separated by 118 nucleotides GFF2045 and GFF2046 are separated by 139 nucleotides GFF2046 and GFF2047 are separated by 48 nucleotides GFF2047 and GFF2048 are separated by 40 nucleotides GFF2048 and GFF2049 are separated by 148 nucleotides GFF2049 and GFF2050 overlap by 4 nucleotides
GFF2044 - Cysteine synthase (EC 2.5.1.47), at 11,250 to 12,221
GFF2044
GFF2045 - hypothetical protein, at 12,340 to 12,465
GFF2045
GFF2046 - Phosphotransferase system, phosphocarrier protein HPr, at 12,605 to 12,862
GFF2046
GFF2047 - Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9), at 12,911 to 14,638
GFF2047
GFF2048 - PTS system, glucose-specific IIA component (EC 2.7.1.69), at 14,679 to 15,188
GFF2048
GFF2049 - FIG01045978: hypothetical protein, at 15,337 to 15,576
GFF2049
GFF2050 - Pyridoxal kinase (EC 2.7.1.35), at 15,573 to 16,439
GFF2050
Position (kb) 12
13
14
15 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 11.925 kb on - strand, within GFF2044 at 12.014 kb on + strand, within GFF2044 at 12.014 kb on + strand, within GFF2044 at 12.197 kb on + strand at 12.364 kb on - strand, within GFF2045 at 12.465 kb on + strand at 12.465 kb on + strand at 12.533 kb on + strand at 13.138 kb on + strand, within GFF2047 at 13.139 kb on - strand, within GFF2047 at 13.139 kb on - strand, within GFF2047 at 13.274 kb on + strand, within GFF2047 at 13.274 kb on + strand, within GFF2047 at 13.299 kb on + strand, within GFF2047 at 13.299 kb on + strand, within GFF2047 at 13.300 kb on - strand, within GFF2047 at 13.300 kb on - strand, within GFF2047 at 13.559 kb on + strand, within GFF2047 at 13.559 kb on + strand, within GFF2047 at 13.592 kb on + strand, within GFF2047 at 13.593 kb on - strand, within GFF2047 at 13.859 kb on + strand, within GFF2047 at 13.940 kb on + strand, within GFF2047 at 14.285 kb on + strand, within GFF2047 at 14.399 kb on - strand, within GFF2047 at 14.510 kb on - strand at 14.660 kb on + strand at 15.099 kb on - strand, within GFF2048 at 15.238 kb on + strand at 15.493 kb on - strand, within GFF2049
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 11,925 - GFF2044 0.69 -0.2 12,014 + GFF2044 0.79 -0.3 12,014 + GFF2044 0.79 +0.1 12,197 + +0.2 12,364 - GFF2045 0.19 -0.3 12,465 + -0.3 12,465 + -0.3 12,533 + -0.6 13,138 + GFF2047 0.13 -1.6 13,139 - GFF2047 0.13 -1.2 13,139 - GFF2047 0.13 -1.9 13,274 + GFF2047 0.21 -2.7 13,274 + GFF2047 0.21 -0.5 13,299 + GFF2047 0.22 -1.5 13,299 + GFF2047 0.22 -2.7 13,300 - GFF2047 0.23 +0.1 13,300 - GFF2047 0.23 -1.5 13,559 + GFF2047 0.38 -3.6 13,559 + GFF2047 0.38 -1.9 13,592 + GFF2047 0.39 -1.5 13,593 - GFF2047 0.39 -2.9 13,859 + GFF2047 0.55 -1.3 13,940 + GFF2047 0.60 -2.8 14,285 + GFF2047 0.80 -2.0 14,399 - GFF2047 0.86 -2.7 14,510 - -3.4 14,660 + -1.2 15,099 - GFF2048 0.82 -1.9 15,238 + +0.3 15,493 - GFF2049 0.65 -0.3
Or see this region's nucleotide sequence