Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF20

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF19 and GFF20 are separated by 70 nucleotidesGFF20 and GFF21 are separated by 189 nucleotides GFF19 - hypothetical protein, at 86 to 208 GFF19 GFF20 - D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase (EC 3.1.1.-), at 279 to 845 GFF20 GFF21 - Methionine ABC transporter ATP-binding protein, at 1,035 to 2,066 GFF21 Position (kb) 0 1Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2at 0.206 kb on + strandat 0.425 kb on + strand, within GFF20at 0.426 kb on - strand, within GFF20at 0.450 kb on - strand, within GFF20at 0.451 kb on + strand, within GFF20at 0.452 kb on - strand, within GFF20at 0.547 kb on - strand, within GFF20at 0.548 kb on + strand, within GFF20at 0.577 kb on + strand, within GFF20at 0.578 kb on - strand, within GFF20at 0.751 kb on - strand, within GFF20at 0.782 kb on - strand, within GFF20at 0.918 kb on - strandat 1.100 kb on + strandat 1.193 kb on - strand, within GFF21at 1.240 kb on + strand, within GFF21at 1.506 kb on - strand, within GFF21at 1.622 kb on + strand, within GFF21at 1.652 kb on + strand, within GFF21at 1.653 kb on - strand, within GFF21at 1.691 kb on + strand, within GFF21at 1.691 kb on + strand, within GFF21

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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206 + +0.2
425 + GFF20 0.26 -1.3
426 - GFF20 0.26 -3.9
450 - GFF20 0.30 -2.2
451 + GFF20 0.30 -3.2
452 - GFF20 0.31 -2.4
547 - GFF20 0.47 -4.8
548 + GFF20 0.47 -1.3
577 + GFF20 0.53 +0.1
578 - GFF20 0.53 -2.7
751 - GFF20 0.83 -2.2
782 - GFF20 0.89 -0.5
918 - -1.3
1,100 + +0.6
1,193 - GFF21 0.15 +0.1
1,240 + GFF21 0.20 -0.1
1,506 - GFF21 0.46 +2.1
1,622 + GFF21 0.57 -0.7
1,652 + GFF21 0.60 +0.4
1,653 - GFF21 0.60 +0.4
1,691 + GFF21 0.64 -0.6
1,691 + GFF21 0.64 +0.2

Or see this region's nucleotide sequence