Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1997 and GFF1998 are separated by 10 nucleotides GFF1998 and GFF1999 are separated by 10 nucleotides GFF1999 and GFF2000 are separated by 111 nucleotides GFF2000 and GFF2001 are separated by 16 nucleotides
GFF1997 - Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1), at 12,485 to 13,219
GFF1997
GFF1998 - Fumarate reductase subunit C, at 13,230 to 13,625
GFF1998
GFF1999 - Fumarate reductase subunit D, at 13,636 to 13,995
GFF1999
GFF2000 - Outer membrane lipoprotein Blc, at 14,107 to 14,640
GFF2000
GFF2001 - Quaternary ammonium compound-resistance protein SugE, at 14,657 to 14,974
GFF2001
Position (kb) 13
14 Strain fitness (log2 ratio) -1
0
1 at 12.738 kb on - strand, within GFF1997 at 12.842 kb on + strand, within GFF1997 at 12.842 kb on + strand, within GFF1997 at 12.847 kb on + strand, within GFF1997 at 12.847 kb on + strand, within GFF1997 at 12.951 kb on - strand, within GFF1997 at 13.238 kb on + strand at 13.251 kb on + strand at 13.251 kb on + strand at 13.252 kb on - strand at 13.252 kb on - strand at 13.305 kb on + strand, within GFF1998 at 13.306 kb on - strand, within GFF1998 at 13.307 kb on + strand, within GFF1998 at 13.308 kb on - strand, within GFF1998 at 13.594 kb on + strand at 13.606 kb on - strand at 13.737 kb on - strand, within GFF1999 at 13.767 kb on - strand, within GFF1999 at 13.874 kb on + strand, within GFF1999 at 13.939 kb on + strand, within GFF1999 at 13.993 kb on + strand at 14.014 kb on + strand at 14.079 kb on + strand at 14.164 kb on - strand, within GFF2000 at 14.199 kb on - strand, within GFF2000 at 14.237 kb on - strand, within GFF2000 at 14.296 kb on + strand, within GFF2000 at 14.296 kb on + strand, within GFF2000 at 14.455 kb on - strand, within GFF2000 at 14.613 kb on - strand at 14.638 kb on + strand at 14.800 kb on - strand, within GFF2001 at 14.957 kb on + strand at 14.974 kb on + strand at 14.988 kb on + strand at 14.992 kb on + strand at 14.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 12,738 - GFF1997 0.34 -0.2 12,842 + GFF1997 0.49 -0.1 12,842 + GFF1997 0.49 -1.3 12,847 + GFF1997 0.49 -0.6 12,847 + GFF1997 0.49 -0.1 12,951 - GFF1997 0.63 +0.2 13,238 + +0.1 13,251 + -0.1 13,251 + -0.1 13,252 - -0.1 13,252 - +0.1 13,305 + GFF1998 0.19 +0.2 13,306 - GFF1998 0.19 +0.2 13,307 + GFF1998 0.19 -1.0 13,308 - GFF1998 0.20 -0.0 13,594 + -1.1 13,606 - -1.5 13,737 - GFF1999 0.28 -1.0 13,767 - GFF1999 0.36 +0.2 13,874 + GFF1999 0.66 +0.0 13,939 + GFF1999 0.84 +0.5 13,993 + +0.4 14,014 + +0.3 14,079 + +0.4 14,164 - GFF2000 0.11 +0.1 14,199 - GFF2000 0.17 +0.6 14,237 - GFF2000 0.24 +0.7 14,296 + GFF2000 0.35 +0.3 14,296 + GFF2000 0.35 -0.2 14,455 - GFF2000 0.65 +0.3 14,613 - -0.1 14,638 + -0.5 14,800 - GFF2001 0.45 -0.2 14,957 + -0.2 14,974 + -0.6 14,988 + -0.9 14,992 + -0.7 14,993 - -0.5
Or see this region's nucleotide sequence