Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF187 and GFF188 are separated by 53 nucleotides GFF188 and GFF189 overlap by 1 nucleotides GFF189 and GFF190 overlap by 4 nucleotides
GFF187 - Outer membrane protein Imp, required for envelope biogenesis / Organic solvent tolerance protein precursor, at 180,920 to 183,280
GFF187
GFF188 - Survival protein SurA precursor (Peptidyl-prolyl cis-trans isomerase SurA) (EC 5.2.1.8), at 183,334 to 184,620
GFF188
GFF189 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 184,620 to 185,609
GFF189
GFF190 - SSU rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase (EC 2.1.1.182), at 185,606 to 186,427
GFF190
Position (kb) 183
184
185 Strain fitness (log2 ratio) -1
0
1 at 183.218 kb on + strand at 183.274 kb on + strand at 183.405 kb on - strand at 183.559 kb on - strand, within GFF188 at 183.706 kb on + strand, within GFF188 at 183.707 kb on - strand, within GFF188 at 183.744 kb on + strand, within GFF188 at 183.744 kb on + strand, within GFF188 at 183.984 kb on + strand, within GFF188 at 183.988 kb on + strand, within GFF188 at 183.989 kb on - strand, within GFF188 at 184.417 kb on - strand, within GFF188 at 184.427 kb on + strand, within GFF188 at 184.485 kb on + strand, within GFF188 at 184.485 kb on + strand, within GFF188 at 184.491 kb on - strand, within GFF188 at 184.530 kb on + strand at 184.598 kb on - strand at 184.603 kb on - strand at 184.603 kb on - strand at 184.614 kb on + strand at 184.906 kb on - strand, within GFF189 at 184.906 kb on - strand, within GFF189 at 184.906 kb on - strand, within GFF189 at 185.178 kb on - strand, within GFF189 at 185.287 kb on + strand, within GFF189 at 185.287 kb on + strand, within GFF189 at 185.288 kb on - strand, within GFF189 at 185.350 kb on + strand, within GFF189 at 185.361 kb on - strand, within GFF189 at 185.383 kb on + strand, within GFF189 at 185.438 kb on - strand, within GFF189
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 183,218 + +0.1 183,274 + -0.3 183,405 - +0.1 183,559 - GFF188 0.17 -0.3 183,706 + GFF188 0.29 -1.4 183,707 - GFF188 0.29 -0.7 183,744 + GFF188 0.32 -0.1 183,744 + GFF188 0.32 -0.8 183,984 + GFF188 0.51 -0.7 183,988 + GFF188 0.51 -0.9 183,989 - GFF188 0.51 -0.9 184,417 - GFF188 0.84 -0.3 184,427 + GFF188 0.85 -0.2 184,485 + GFF188 0.89 +0.3 184,485 + GFF188 0.89 -0.5 184,491 - GFF188 0.90 -1.7 184,530 + -0.2 184,598 - -0.6 184,603 - -0.1 184,603 - -0.1 184,614 + +0.5 184,906 - GFF189 0.29 -0.7 184,906 - GFF189 0.29 +0.2 184,906 - GFF189 0.29 -0.6 185,178 - GFF189 0.56 -0.2 185,287 + GFF189 0.67 -0.1 185,287 + GFF189 0.67 -0.0 185,288 - GFF189 0.67 -1.0 185,350 + GFF189 0.74 +0.4 185,361 - GFF189 0.75 -0.3 185,383 + GFF189 0.77 -0.1 185,438 - GFF189 0.83 -0.4
Or see this region's nucleotide sequence