Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1830 and GFF1831 are separated by 16 nucleotides GFF1831 and GFF1832 are separated by 102 nucleotides GFF1832 and GFF1833 are separated by 117 nucleotides
GFF1830 - Two-component sensor protein RcsD (EC 2.7.3.-), at 13,701 to 16,370
GFF1830
GFF1831 - DNA-binding capsular synthesis response regulator RcsB, at 16,387 to 17,037
GFF1831
GFF1832 - Two-component sensor protein RcsC (EC 2.7.3.-), at 17,140 to 19,986
GFF1832
GFF1833 - DNA gyrase subunit A (EC 5.99.1.3), at 20,104 to 22,740
GFF1833
Position (kb) 17
18
19
20 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 16.163 kb on + strand at 16.173 kb on + strand at 16.385 kb on - strand at 16.396 kb on - strand at 16.431 kb on - strand at 16.923 kb on + strand, within GFF1831 at 17.046 kb on + strand at 17.047 kb on - strand at 17.075 kb on + strand at 17.377 kb on - strand at 17.441 kb on + strand, within GFF1832 at 17.591 kb on + strand, within GFF1832 at 17.592 kb on - strand, within GFF1832 at 17.595 kb on + strand, within GFF1832 at 17.596 kb on - strand, within GFF1832 at 17.596 kb on - strand, within GFF1832 at 17.903 kb on + strand, within GFF1832 at 18.140 kb on + strand, within GFF1832 at 18.220 kb on - strand, within GFF1832 at 18.277 kb on - strand, within GFF1832 at 18.320 kb on + strand, within GFF1832 at 18.320 kb on + strand, within GFF1832 at 18.333 kb on + strand, within GFF1832 at 18.333 kb on + strand, within GFF1832 at 18.380 kb on - strand, within GFF1832 at 18.655 kb on + strand, within GFF1832 at 19.149 kb on + strand, within GFF1832 at 19.251 kb on + strand, within GFF1832 at 19.475 kb on - strand, within GFF1832 at 19.569 kb on - strand, within GFF1832 at 19.824 kb on + strand at 19.881 kb on - strand at 19.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 16,163 + -3.5 16,173 + -1.3 16,385 - -2.5 16,396 - -3.5 16,431 - -2.5 16,923 + GFF1831 0.82 -2.3 17,046 + -1.5 17,047 - -0.7 17,075 + -0.5 17,377 - -1.5 17,441 + GFF1832 0.11 -1.5 17,591 + GFF1832 0.16 -3.0 17,592 - GFF1832 0.16 -2.1 17,595 + GFF1832 0.16 -2.8 17,596 - GFF1832 0.16 -0.9 17,596 - GFF1832 0.16 -4.0 17,903 + GFF1832 0.27 -3.7 18,140 + GFF1832 0.35 -1.7 18,220 - GFF1832 0.38 -4.1 18,277 - GFF1832 0.40 -2.5 18,320 + GFF1832 0.41 -4.1 18,320 + GFF1832 0.41 -2.7 18,333 + GFF1832 0.42 -2.5 18,333 + GFF1832 0.42 -2.0 18,380 - GFF1832 0.44 -3.0 18,655 + GFF1832 0.53 -3.1 19,149 + GFF1832 0.71 -3.0 19,251 + GFF1832 0.74 -3.8 19,475 - GFF1832 0.82 -2.9 19,569 - GFF1832 0.85 +0.0 19,824 + -3.0 19,881 - -3.3 19,937 - -1.3
Or see this region's nucleotide sequence