Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1634

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1633 and GFF1634 are separated by 72 nucleotidesGFF1634 and GFF1635 are separated by 116 nucleotides GFF1633 - Pyruvate kinase (EC 2.7.1.40), at 18,898 to 20,340 GFF1633 GFF1634 - Lipid A biosynthesis (KDO) 2-(lauroyl)-lipid IVA acyltransferase (EC 2.3.1.-), at 20,413 to 21,384 GFF1634 GFF1635 - Cell wall endopeptidase, family M23/M37, at 21,501 to 22,820 GFF1635 Position (kb) 20 21 22Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 19.472 kb on - strand, within GFF1633at 20.323 kb on - strandat 20.339 kb on - strandat 20.369 kb on + strandat 20.498 kb on + strandat 20.625 kb on + strand, within GFF1634at 20.634 kb on - strand, within GFF1634at 20.634 kb on - strand, within GFF1634at 20.708 kb on + strand, within GFF1634at 20.709 kb on - strand, within GFF1634at 20.769 kb on - strand, within GFF1634at 20.874 kb on - strand, within GFF1634at 20.899 kb on - strand, within GFF1634at 21.357 kb on - strandat 21.425 kb on - strandat 21.429 kb on + strandat 21.547 kb on + strandat 21.630 kb on + strandat 21.631 kb on - strandat 21.745 kb on + strand, within GFF1635at 21.757 kb on + strand, within GFF1635at 21.970 kb on - strand, within GFF1635at 22.006 kb on + strand, within GFF1635at 22.040 kb on - strand, within GFF1635at 22.040 kb on - strand, within GFF1635at 22.040 kb on - strand, within GFF1635at 22.242 kb on - strand, within GFF1635at 22.242 kb on - strand, within GFF1635at 22.254 kb on - strand, within GFF1635at 22.331 kb on - strand, within GFF1635

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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19,472 - GFF1633 0.40 -1.5
20,323 - -0.0
20,339 - -1.0
20,369 + +0.1
20,498 + +0.9
20,625 + GFF1634 0.22 +0.1
20,634 - GFF1634 0.23 -2.2
20,634 - GFF1634 0.23 -0.9
20,708 + GFF1634 0.30 -1.5
20,709 - GFF1634 0.30 -1.9
20,769 - GFF1634 0.37 -2.2
20,874 - GFF1634 0.47 +0.1
20,899 - GFF1634 0.50 -3.1
21,357 - +0.9
21,425 - +0.6
21,429 + -1.0
21,547 + -0.6
21,630 + -0.1
21,631 - +0.8
21,745 + GFF1635 0.18 -0.9
21,757 + GFF1635 0.19 +1.9
21,970 - GFF1635 0.36 +0.4
22,006 + GFF1635 0.38 +0.0
22,040 - GFF1635 0.41 -0.6
22,040 - GFF1635 0.41 +0.3
22,040 - GFF1635 0.41 -0.7
22,242 - GFF1635 0.56 +0.2
22,242 - GFF1635 0.56 +1.1
22,254 - GFF1635 0.57 +0.3
22,331 - GFF1635 0.63 +0.5

Or see this region's nucleotide sequence