Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1439 and GFF1440 are separated by 622 nucleotides GFF1440 and GFF1441 overlap by 13 nucleotides GFF1441 and GFF1442 are separated by 86 nucleotides
GFF1439 - YjcB protein, at 101,668 to 101,889
GFF1439
GFF1440 - FIG00638940: hypothetical protein, at 102,512 to 104,113
GFF1440
GFF1441 - Regulatory protein SoxS, at 104,101 to 104,424
GFF1441
GFF1442 - Redox-sensitive transcriptional activator SoxR, at 104,511 to 104,969
GFF1442
Position (kb) 102
103
104
105 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 101.767 kb on - strand, within GFF1439 at 101.770 kb on - strand, within GFF1439 at 101.777 kb on - strand, within GFF1439 at 101.842 kb on - strand, within GFF1439 at 101.842 kb on - strand, within GFF1439 at 101.844 kb on - strand, within GFF1439 at 101.967 kb on + strand at 102.307 kb on - strand at 102.636 kb on + strand at 102.879 kb on - strand, within GFF1440 at 102.906 kb on - strand, within GFF1440 at 102.951 kb on - strand, within GFF1440 at 102.952 kb on + strand, within GFF1440 at 102.952 kb on + strand, within GFF1440 at 102.952 kb on + strand, within GFF1440 at 103.040 kb on + strand, within GFF1440 at 103.041 kb on - strand, within GFF1440 at 103.041 kb on - strand, within GFF1440 at 103.041 kb on - strand, within GFF1440 at 103.098 kb on + strand, within GFF1440 at 103.101 kb on + strand, within GFF1440 at 103.217 kb on + strand, within GFF1440 at 103.270 kb on + strand, within GFF1440 at 103.288 kb on + strand, within GFF1440 at 103.313 kb on + strand, within GFF1440 at 103.317 kb on - strand, within GFF1440 at 103.317 kb on - strand, within GFF1440 at 103.410 kb on + strand, within GFF1440 at 103.410 kb on + strand, within GFF1440 at 103.411 kb on - strand, within GFF1440 at 103.411 kb on - strand, within GFF1440 at 103.521 kb on - strand, within GFF1440 at 103.548 kb on + strand, within GFF1440 at 103.580 kb on + strand, within GFF1440 at 103.581 kb on - strand, within GFF1440 at 103.581 kb on - strand, within GFF1440 at 103.589 kb on - strand, within GFF1440 at 103.681 kb on - strand, within GFF1440 at 103.709 kb on - strand, within GFF1440 at 103.825 kb on + strand, within GFF1440 at 103.825 kb on + strand, within GFF1440 at 103.846 kb on + strand, within GFF1440 at 103.874 kb on - strand, within GFF1440 at 103.904 kb on - strand, within GFF1440 at 103.904 kb on - strand, within GFF1440 at 103.904 kb on - strand, within GFF1440 at 104.001 kb on - strand at 104.152 kb on + strand, within GFF1441 at 104.177 kb on - strand, within GFF1441 at 104.180 kb on + strand, within GFF1441 at 104.316 kb on - strand, within GFF1441 at 104.373 kb on + strand, within GFF1441 at 104.437 kb on + strand at 104.542 kb on + strand at 104.571 kb on - strand, within GFF1442 at 104.676 kb on + strand, within GFF1442 at 104.745 kb on - strand, within GFF1442 at 104.762 kb on + strand, within GFF1442 at 104.762 kb on + strand, within GFF1442 at 104.763 kb on - strand, within GFF1442 at 104.763 kb on - strand, within GFF1442 at 104.763 kb on - strand, within GFF1442 at 104.815 kb on + strand, within GFF1442 at 104.832 kb on - strand, within GFF1442 at 104.967 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 101,767 - GFF1439 0.45 -0.3 101,770 - GFF1439 0.46 +0.5 101,777 - GFF1439 0.49 -0.6 101,842 - GFF1439 0.78 -0.6 101,842 - GFF1439 0.78 -0.3 101,844 - GFF1439 0.79 +0.2 101,967 + +0.4 102,307 - -0.1 102,636 + -1.8 102,879 - GFF1440 0.23 -1.1 102,906 - GFF1440 0.25 -2.5 102,951 - GFF1440 0.27 -1.4 102,952 + GFF1440 0.27 -1.7 102,952 + GFF1440 0.27 -0.8 102,952 + GFF1440 0.27 -1.1 103,040 + GFF1440 0.33 -3.4 103,041 - GFF1440 0.33 -0.6 103,041 - GFF1440 0.33 -0.6 103,041 - GFF1440 0.33 -1.3 103,098 + GFF1440 0.37 -4.6 103,101 + GFF1440 0.37 -1.6 103,217 + GFF1440 0.44 -2.1 103,270 + GFF1440 0.47 -0.9 103,288 + GFF1440 0.48 -0.8 103,313 + GFF1440 0.50 -1.4 103,317 - GFF1440 0.50 -1.0 103,317 - GFF1440 0.50 -1.0 103,410 + GFF1440 0.56 -1.6 103,410 + GFF1440 0.56 -1.7 103,411 - GFF1440 0.56 -0.9 103,411 - GFF1440 0.56 -0.8 103,521 - GFF1440 0.63 -1.3 103,548 + GFF1440 0.65 -2.0 103,580 + GFF1440 0.67 -1.4 103,581 - GFF1440 0.67 -1.5 103,581 - GFF1440 0.67 -1.2 103,589 - GFF1440 0.67 -1.2 103,681 - GFF1440 0.73 -1.1 103,709 - GFF1440 0.75 -3.8 103,825 + GFF1440 0.82 -1.8 103,825 + GFF1440 0.82 -1.1 103,846 + GFF1440 0.83 -1.0 103,874 - GFF1440 0.85 -2.5 103,904 - GFF1440 0.87 -2.3 103,904 - GFF1440 0.87 +0.0 103,904 - GFF1440 0.87 -0.7 104,001 - -2.6 104,152 + GFF1441 0.16 +0.2 104,177 - GFF1441 0.23 -0.2 104,180 + GFF1441 0.24 -0.2 104,316 - GFF1441 0.66 +1.0 104,373 + GFF1441 0.84 -0.5 104,437 + -0.2 104,542 + +1.0 104,571 - GFF1442 0.13 -0.3 104,676 + GFF1442 0.36 -0.7 104,745 - GFF1442 0.51 -0.1 104,762 + GFF1442 0.55 -0.2 104,762 + GFF1442 0.55 +0.2 104,763 - GFF1442 0.55 -0.3 104,763 - GFF1442 0.55 -0.2 104,763 - GFF1442 0.55 +0.3 104,815 + GFF1442 0.66 +0.3 104,832 - GFF1442 0.70 -1.3 104,967 + -0.0
Or see this region's nucleotide sequence