Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1411

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1410 and GFF1411 are separated by 170 nucleotidesGFF1411 and GFF1412 are separated by 108 nucleotidesGFF1412 and GFF1413 are separated by 178 nucleotides GFF1410 - Glycerol-3-phosphate acyltransferase (EC 2.3.1.15), at 56,311 to 58,731 GFF1410 GFF1411 - Diacylglycerol kinase (EC 2.7.1.107), at 58,902 to 59,270 GFF1411 GFF1412 - SOS-response repressor and protease LexA (EC 3.4.21.88), at 59,379 to 59,987 GFF1412 GFF1413 - DNA-damage-inducible protein F, at 60,166 to 61,491 GFF1413 Position (kb) 58 59 60Strain fitness (log2 ratio) -2 -1 0 1at 58.862 kb on - strandat 58.862 kb on - strandat 58.862 kb on - strandat 58.862 kb on - strandat 58.864 kb on - strandat 58.911 kb on - strandat 58.951 kb on - strand, within GFF1411at 59.080 kb on + strand, within GFF1411at 59.092 kb on + strand, within GFF1411at 59.097 kb on - strand, within GFF1411at 59.097 kb on - strand, within GFF1411at 59.127 kb on - strand, within GFF1411at 59.209 kb on + strand, within GFF1411at 59.209 kb on + strand, within GFF1411at 59.986 kb on - strandat 60.109 kb on + strandat 60.228 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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58,862 - +0.1
58,862 - -0.4
58,862 - -0.1
58,862 - -0.2
58,864 - -1.7
58,911 - -1.5
58,951 - GFF1411 0.13 -0.5
59,080 + GFF1411 0.48 -2.3
59,092 + GFF1411 0.51 -2.1
59,097 - GFF1411 0.53 -1.7
59,097 - GFF1411 0.53 -0.5
59,127 - GFF1411 0.61 -0.3
59,209 + GFF1411 0.83 +0.2
59,209 + GFF1411 0.83 -0.6
59,986 - -0.6
60,109 + +0.8
60,228 + -0.1

Or see this region's nucleotide sequence