Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1388 and GFF1389 overlap by 11 nucleotides GFF1389 and GFF1390 overlap by 4 nucleotides GFF1390 and GFF1391 are separated by 159 nucleotides GFF1391 and GFF1392 are separated by 12 nucleotides
GFF1388 - Phage tail sheath monomer, at 34,094 to 35,521
GFF1388
GFF1389 - FIG074102: hypothetical protein, at 35,511 to 35,708
GFF1389
GFF1390 - Phage protein, at 35,705 to 36,160
GFF1390
GFF1391 - Putative inner membrane protein, at 36,320 to 36,634
GFF1391
GFF1392 - Phage lysin, at 36,647 to 37,252
GFF1392
Position (kb) 35
36 Strain fitness (log2 ratio) -1
0
1 at 34.762 kb on + strand, within GFF1388 at 34.762 kb on + strand, within GFF1388 at 34.830 kb on + strand, within GFF1388 at 34.831 kb on - strand, within GFF1388 at 34.834 kb on + strand, within GFF1388 at 34.835 kb on - strand, within GFF1388 at 35.034 kb on + strand, within GFF1388 at 35.049 kb on + strand, within GFF1388 at 35.050 kb on - strand, within GFF1388 at 35.119 kb on - strand, within GFF1388 at 35.134 kb on + strand, within GFF1388 at 35.137 kb on - strand, within GFF1388 at 35.275 kb on + strand, within GFF1388 at 35.495 kb on + strand at 35.496 kb on - strand at 35.496 kb on - strand at 35.513 kb on - strand at 35.646 kb on + strand, within GFF1389 at 35.647 kb on - strand, within GFF1389 at 35.833 kb on - strand, within GFF1390 at 35.919 kb on - strand, within GFF1390 at 35.930 kb on + strand, within GFF1390 at 35.931 kb on - strand, within GFF1390 at 36.095 kb on - strand, within GFF1390 at 36.120 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 34,762 + GFF1388 0.47 -0.1 34,762 + GFF1388 0.47 +0.1 34,830 + GFF1388 0.52 -1.0 34,831 - GFF1388 0.52 +0.2 34,834 + GFF1388 0.52 -1.1 34,835 - GFF1388 0.52 +0.4 35,034 + GFF1388 0.66 -0.4 35,049 + GFF1388 0.67 +0.2 35,050 - GFF1388 0.67 -0.0 35,119 - GFF1388 0.72 +0.2 35,134 + GFF1388 0.73 +0.5 35,137 - GFF1388 0.73 -0.4 35,275 + GFF1388 0.83 +0.6 35,495 + +0.7 35,496 - +0.2 35,496 - -0.4 35,513 - -0.4 35,646 + GFF1389 0.68 -0.6 35,647 - GFF1389 0.69 -0.5 35,833 - GFF1390 0.28 +0.1 35,919 - GFF1390 0.47 +0.8 35,930 + GFF1390 0.49 +0.8 35,931 - GFF1390 0.50 +0.2 36,095 - GFF1390 0.86 +0.5 36,120 - +0.0
Or see this region's nucleotide sequence