Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1234

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1233 and GFF1234 are separated by 864 nucleotidesGFF1234 and GFF1235 are separated by 53 nucleotides GFF1233 - Transposase, at 66,121 to 66,363 GFF1233 GFF1234 - hypothetical protein, at 67,228 to 67,347 GFF1234 GFF1235 - 27.5 kDa virulence protein, at 67,401 to 67,988 GFF1235 Position (kb) 67 68Strain fitness (log2 ratio) -2 -1 0 1at 66.287 kb on - strand, within GFF1233at 66.318 kb on - strand, within GFF1233at 66.334 kb on + strand, within GFF1233at 66.335 kb on - strand, within GFF1233at 66.335 kb on - strand, within GFF1233at 66.397 kb on + strandat 66.398 kb on - strandat 67.243 kb on - strand, within GFF1234at 67.488 kb on - strand, within GFF1235at 67.488 kb on - strand, within GFF1235at 67.590 kb on - strand, within GFF1235at 67.624 kb on + strand, within GFF1235at 67.914 kb on - strand, within GFF1235at 68.115 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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66,287 - GFF1233 0.68 -2.6
66,318 - GFF1233 0.81 -0.3
66,334 + GFF1233 0.88 -0.9
66,335 - GFF1233 0.88 -0.6
66,335 - GFF1233 0.88 -0.2
66,397 + -0.1
66,398 - -0.6
67,243 - GFF1234 0.12 -0.6
67,488 - GFF1235 0.15 -0.7
67,488 - GFF1235 0.15 -1.3
67,590 - GFF1235 0.32 +0.4
67,624 + GFF1235 0.38 -0.6
67,914 - GFF1235 0.87 -1.2
68,115 - -0.3

Or see this region's nucleotide sequence