Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1219 and GFF1220 are separated by 250 nucleotides GFF1220 and GFF1221 are separated by 253 nucleotides GFF1221 and GFF1222 are separated by 326 nucleotides GFF1222 and GFF1223 are separated by 1 nucleotides GFF1223 and GFF1224 are separated by 1 nucleotides
GFF1219 - FIG01048676: hypothetical protein, at 58,758 to 58,871
GFF1219
GFF1220 - FIG01047634: hypothetical protein, at 59,122 to 59,610
GFF1220
GFF1221 - hypothetical protein, at 59,864 to 59,992
GFF1221
GFF1222 - CcdA protein (antitoxin to CcdB), at 60,319 to 60,537
GFF1222
GFF1223 - CcdB toxin protein, at 60,539 to 60,844
GFF1223
GFF1224 - putative cytoplasmic protein, at 60,846 to 61,136
GFF1224
Position (kb) 59
60 Strain fitness (log2 ratio) -2
-1
0
1 at 58.879 kb on + strand at 58.905 kb on + strand at 58.906 kb on - strand at 58.916 kb on + strand at 58.917 kb on - strand at 59.032 kb on - strand at 59.054 kb on + strand at 59.055 kb on - strand at 59.061 kb on + strand at 59.062 kb on - strand at 59.085 kb on + strand at 59.096 kb on - strand at 59.097 kb on + strand at 59.098 kb on - strand at 59.098 kb on - strand at 59.103 kb on + strand at 59.182 kb on + strand, within GFF1220 at 59.182 kb on + strand, within GFF1220 at 59.182 kb on + strand, within GFF1220 at 59.182 kb on + strand, within GFF1220 at 59.182 kb on + strand, within GFF1220 at 59.182 kb on + strand, within GFF1220 at 59.183 kb on - strand, within GFF1220 at 59.291 kb on - strand, within GFF1220 at 59.316 kb on + strand, within GFF1220 at 59.367 kb on + strand, within GFF1220 at 59.482 kb on + strand, within GFF1220 at 59.483 kb on - strand, within GFF1220 at 59.541 kb on - strand, within GFF1220 at 59.544 kb on - strand, within GFF1220 at 59.552 kb on + strand, within GFF1220 at 59.552 kb on + strand, within GFF1220 at 59.583 kb on + strand at 59.584 kb on - strand at 59.597 kb on - strand at 59.597 kb on - strand at 59.623 kb on - strand at 59.623 kb on - strand at 59.628 kb on + strand at 59.628 kb on + strand at 59.628 kb on + strand at 59.629 kb on - strand at 59.667 kb on - strand at 59.801 kb on + strand at 59.857 kb on + strand at 59.900 kb on - strand, within GFF1221 at 60.170 kb on - strand at 60.174 kb on - strand at 60.186 kb on + strand at 60.221 kb on + strand at 60.275 kb on + strand at 60.358 kb on + strand, within GFF1222 at 60.377 kb on - strand, within GFF1222 at 60.471 kb on + strand, within GFF1222 at 60.472 kb on - strand, within GFF1222 at 60.472 kb on - strand, within GFF1222 at 60.472 kb on - strand, within GFF1222 at 60.493 kb on - strand, within GFF1222 at 60.494 kb on + strand, within GFF1222 at 60.548 kb on - strand at 60.548 kb on - strand at 60.554 kb on + strand at 60.555 kb on - strand at 60.703 kb on + strand, within GFF1223 at 60.875 kb on + strand, within GFF1224 at 60.880 kb on + strand, within GFF1224
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 58,879 + +0.2 58,905 + -0.6 58,906 - -0.5 58,916 + -0.2 58,917 - +0.8 59,032 - -0.2 59,054 + -1.1 59,055 - -0.5 59,061 + -0.2 59,062 - -1.9 59,085 + +0.4 59,096 - -0.2 59,097 + +0.3 59,098 - +0.8 59,098 - +0.3 59,103 + +0.1 59,182 + GFF1220 0.12 +0.2 59,182 + GFF1220 0.12 +0.4 59,182 + GFF1220 0.12 +0.1 59,182 + GFF1220 0.12 +0.0 59,182 + GFF1220 0.12 +0.1 59,182 + GFF1220 0.12 -0.3 59,183 - GFF1220 0.12 +1.2 59,291 - GFF1220 0.35 +0.3 59,316 + GFF1220 0.40 -0.6 59,367 + GFF1220 0.50 -0.8 59,482 + GFF1220 0.74 -0.4 59,483 - GFF1220 0.74 -0.2 59,541 - GFF1220 0.86 -0.2 59,544 - GFF1220 0.86 -0.1 59,552 + GFF1220 0.88 +0.3 59,552 + GFF1220 0.88 +0.2 59,583 + -0.2 59,584 - +0.1 59,597 - -0.4 59,597 - -0.8 59,623 - -0.7 59,623 - +0.2 59,628 + -0.4 59,628 + +0.0 59,628 + +0.3 59,629 - -0.3 59,667 - +1.0 59,801 + +0.0 59,857 + -0.1 59,900 - GFF1221 0.28 -0.6 60,170 - -0.4 60,174 - +0.6 60,186 + -1.0 60,221 + +0.5 60,275 + -0.9 60,358 + GFF1222 0.18 -0.2 60,377 - GFF1222 0.26 -0.0 60,471 + GFF1222 0.69 +0.6 60,472 - GFF1222 0.70 +0.9 60,472 - GFF1222 0.70 +0.2 60,472 - GFF1222 0.70 -0.6 60,493 - GFF1222 0.79 -1.0 60,494 + GFF1222 0.80 +0.1 60,548 - -0.5 60,548 - -0.3 60,554 + +1.1 60,555 - -0.4 60,703 + GFF1223 0.54 +0.0 60,875 + GFF1224 0.10 +0.1 60,880 + GFF1224 0.12 -0.0
Or see this region's nucleotide sequence