Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1171 and GFF1172 overlap by 16 nucleotides GFF1172 and GFF1173 overlap by 33 nucleotides GFF1173 and GFF1174 are separated by 42 nucleotides GFF1174 and GFF1175 overlap by 4 nucleotides
GFF1171 - Conjugative transfer protein PSLT093, at 19,592 to 19,831
GFF1171
GFF1172 - IncF plasmid conjugative transfer protein TrbC, at 19,816 to 20,454
GFF1172
GFF1173 - hypothetical protein, at 20,422 to 20,586
GFF1173
GFF1174 - FIG00642834: hypothetical protein, at 20,629 to 20,910
GFF1174
GFF1175 - IncF plasmid conjugative transfer protein TraN, at 20,907 to 22,733
GFF1175
Position (kb) 20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 19.697 kb on - strand, within GFF1171 at 19.765 kb on + strand, within GFF1171 at 19.766 kb on - strand, within GFF1171 at 19.798 kb on + strand, within GFF1171 at 19.798 kb on + strand, within GFF1171 at 19.891 kb on - strand, within GFF1172 at 19.893 kb on + strand, within GFF1172 at 19.894 kb on - strand, within GFF1172 at 19.894 kb on - strand, within GFF1172 at 20.162 kb on + strand, within GFF1172 at 20.288 kb on - strand, within GFF1172 at 20.288 kb on - strand, within GFF1172 at 20.288 kb on - strand, within GFF1172 at 20.321 kb on - strand, within GFF1172 at 20.321 kb on - strand, within GFF1172 at 20.335 kb on + strand, within GFF1172 at 20.335 kb on + strand, within GFF1172 at 20.443 kb on - strand at 20.752 kb on + strand, within GFF1174 at 20.817 kb on + strand, within GFF1174 at 20.850 kb on + strand, within GFF1174 at 20.850 kb on + strand, within GFF1174 at 20.850 kb on + strand, within GFF1174 at 20.915 kb on + strand at 20.916 kb on - strand at 20.920 kb on + strand at 21.056 kb on + strand at 21.057 kb on - strand at 21.068 kb on + strand at 21.275 kb on + strand, within GFF1175 at 21.275 kb on + strand, within GFF1175 at 21.275 kb on + strand, within GFF1175 at 21.276 kb on - strand, within GFF1175 at 21.276 kb on - strand, within GFF1175 at 21.340 kb on - strand, within GFF1175 at 21.341 kb on + strand, within GFF1175 at 21.341 kb on + strand, within GFF1175 at 21.341 kb on + strand, within GFF1175 at 21.341 kb on + strand, within GFF1175 at 21.372 kb on + strand, within GFF1175 at 21.373 kb on - strand, within GFF1175 at 21.373 kb on - strand, within GFF1175 at 21.432 kb on + strand, within GFF1175 at 21.432 kb on + strand, within GFF1175 at 21.450 kb on - strand, within GFF1175 at 21.487 kb on + strand, within GFF1175 at 21.546 kb on + strand, within GFF1175 at 21.547 kb on - strand, within GFF1175 at 21.573 kb on - strand, within GFF1175 at 21.578 kb on + strand, within GFF1175 at 21.578 kb on + strand, within GFF1175 at 21.611 kb on + strand, within GFF1175 at 21.611 kb on + strand, within GFF1175 at 21.612 kb on - strand, within GFF1175 at 21.612 kb on - strand, within GFF1175 at 21.612 kb on - strand, within GFF1175 at 21.612 kb on - strand, within GFF1175 at 21.644 kb on + strand, within GFF1175 at 21.645 kb on - strand, within GFF1175 at 21.645 kb on - strand, within GFF1175 at 21.686 kb on + strand, within GFF1175 at 21.773 kb on - strand, within GFF1175
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 19,697 - GFF1171 0.44 -0.4 19,765 + GFF1171 0.72 +0.2 19,766 - GFF1171 0.72 -0.1 19,798 + GFF1171 0.86 +1.4 19,798 + GFF1171 0.86 -0.0 19,891 - GFF1172 0.12 -0.0 19,893 + GFF1172 0.12 -0.3 19,894 - GFF1172 0.12 -0.7 19,894 - GFF1172 0.12 -0.1 20,162 + GFF1172 0.54 -0.6 20,288 - GFF1172 0.74 -3.4 20,288 - GFF1172 0.74 +0.0 20,288 - GFF1172 0.74 +0.6 20,321 - GFF1172 0.79 +0.1 20,321 - GFF1172 0.79 -0.8 20,335 + GFF1172 0.81 -1.9 20,335 + GFF1172 0.81 -0.2 20,443 - +0.6 20,752 + GFF1174 0.44 +0.0 20,817 + GFF1174 0.67 -1.4 20,850 + GFF1174 0.78 -0.6 20,850 + GFF1174 0.78 -0.1 20,850 + GFF1174 0.78 -1.0 20,915 + +0.1 20,916 - +0.4 20,920 + +0.5 21,056 + -0.2 21,057 - +0.5 21,068 + -0.5 21,275 + GFF1175 0.20 +0.3 21,275 + GFF1175 0.20 -0.2 21,275 + GFF1175 0.20 -0.9 21,276 - GFF1175 0.20 -0.3 21,276 - GFF1175 0.20 +0.2 21,340 - GFF1175 0.24 +0.3 21,341 + GFF1175 0.24 -0.6 21,341 + GFF1175 0.24 +0.1 21,341 + GFF1175 0.24 -0.0 21,341 + GFF1175 0.24 -1.9 21,372 + GFF1175 0.25 +0.1 21,373 - GFF1175 0.26 -1.3 21,373 - GFF1175 0.26 +0.9 21,432 + GFF1175 0.29 +0.6 21,432 + GFF1175 0.29 -0.7 21,450 - GFF1175 0.30 +0.1 21,487 + GFF1175 0.32 -0.9 21,546 + GFF1175 0.35 +0.5 21,547 - GFF1175 0.35 -1.6 21,573 - GFF1175 0.36 -0.3 21,578 + GFF1175 0.37 +0.0 21,578 + GFF1175 0.37 +0.4 21,611 + GFF1175 0.39 +0.3 21,611 + GFF1175 0.39 +0.9 21,612 - GFF1175 0.39 +0.9 21,612 - GFF1175 0.39 -0.1 21,612 - GFF1175 0.39 -0.3 21,612 - GFF1175 0.39 +0.1 21,644 + GFF1175 0.40 -0.9 21,645 - GFF1175 0.40 +1.8 21,645 - GFF1175 0.40 +0.2 21,686 + GFF1175 0.43 +0.2 21,773 - GFF1175 0.47 -0.5
Or see this region's nucleotide sequence