Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF115

Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF114 and GFF115 are separated by 60 nucleotidesGFF115 and GFF116 are separated by 466 nucleotides GFF114 - HNH endonuclease, at 99,733 to 100,575 GFF114 GFF115 - Aconitate hydratase 2 (EC 4.2.1.3) @ 2-methylisocitrate dehydratase (EC 4.2.1.99), at 100,636 to 103,155 GFF115 GFF116 - FIG01200701: possible membrane protein, at 103,622 to 105,244 GFF116 Position (kb) 100 101 102 103 104Strain fitness (log2 ratio) -3 -2 -1 0 1at 99.693 kb on - strandat 100.716 kb on + strandat 100.749 kb on - strandat 100.866 kb on + strandat 101.053 kb on - strand, within GFF115at 101.053 kb on - strand, within GFF115at 101.379 kb on - strand, within GFF115at 101.413 kb on - strand, within GFF115at 101.779 kb on - strand, within GFF115at 101.842 kb on - strand, within GFF115at 102.042 kb on + strand, within GFF115at 102.287 kb on - strand, within GFF115at 102.394 kb on - strand, within GFF115at 103.097 kb on - strandat 103.208 kb on - strandat 103.314 kb on - strandat 103.317 kb on + strandat 103.326 kb on - strandat 103.344 kb on - strandat 103.430 kb on + strandat 103.461 kb on + strandat 103.469 kb on + strandat 103.498 kb on - strandat 103.519 kb on - strandat 103.545 kb on + strandat 103.618 kb on + strandat 103.925 kb on - strand, within GFF116

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI
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99,693 - -0.3
100,716 + +0.3
100,749 - -0.9
100,866 + -0.3
101,053 - GFF115 0.17 +0.1
101,053 - GFF115 0.17 -0.2
101,379 - GFF115 0.29 -0.9
101,413 - GFF115 0.31 -0.9
101,779 - GFF115 0.45 -0.5
101,842 - GFF115 0.48 -1.4
102,042 + GFF115 0.56 -3.2
102,287 - GFF115 0.66 -2.7
102,394 - GFF115 0.70 -1.1
103,097 - -1.1
103,208 - -0.7
103,314 - +0.2
103,317 + -1.2
103,326 - -0.2
103,344 - -0.0
103,430 + -0.4
103,461 + -0.3
103,469 + +0.3
103,498 - -0.8
103,519 - +0.1
103,545 + -0.7
103,618 + -0.5
103,925 - GFF116 0.19 +0.5

Or see this region's nucleotide sequence