Experiment: LB_plus_SM_buffer with 6D2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF108 and GFF109 are separated by 29 nucleotides GFF109 and GFF110 are separated by 9 nucleotides GFF110 and GFF111 overlap by 11 nucleotides
GFF108 - S-adenosylmethionine decarboxylase proenzyme (EC 4.1.1.50), prokaryotic class 1A, at 94,095 to 94,889
GFF108
GFF109 - DeoR family transcriptional regulator probably related to glycerate, glycolaldehyde metabolism, at 94,919 to 95,686
GFF109
GFF110 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 95,696 to 96,679
GFF110
GFF111 - Candidate type III effector Hop protein, at 96,669 to 97,940
GFF111
Position (kb) 95
96
97 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 94.709 kb on - strand, within GFF108 at 94.751 kb on + strand, within GFF108 at 94.773 kb on + strand, within GFF108 at 94.773 kb on + strand, within GFF108 at 94.774 kb on - strand, within GFF108 at 95.055 kb on + strand, within GFF109 at 95.055 kb on + strand, within GFF109 at 95.059 kb on + strand, within GFF109 at 95.059 kb on + strand, within GFF109 at 95.179 kb on + strand, within GFF109 at 95.649 kb on + strand at 95.896 kb on + strand, within GFF110 at 95.962 kb on + strand, within GFF110 at 96.104 kb on - strand, within GFF110 at 96.144 kb on - strand, within GFF110 at 96.144 kb on - strand, within GFF110 at 96.149 kb on - strand, within GFF110 at 96.215 kb on + strand, within GFF110 at 96.384 kb on - strand, within GFF110 at 96.683 kb on - strand at 96.683 kb on - strand at 96.697 kb on + strand at 96.720 kb on - strand at 97.399 kb on + strand, within GFF111 at 97.407 kb on - strand, within GFF111 at 97.410 kb on + strand, within GFF111 at 97.411 kb on - strand, within GFF111 at 97.419 kb on + strand, within GFF111 at 97.456 kb on + strand, within GFF111 at 97.486 kb on - strand, within GFF111 at 97.673 kb on + strand, within GFF111
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6D2_phage 15 MOI remove 94,709 - GFF108 0.77 -0.2 94,751 + GFF108 0.83 +0.6 94,773 + GFF108 0.85 -0.3 94,773 + GFF108 0.85 -0.2 94,774 - GFF108 0.85 +0.5 95,055 + GFF109 0.18 -0.7 95,055 + GFF109 0.18 +0.8 95,059 + GFF109 0.18 -0.1 95,059 + GFF109 0.18 -1.1 95,179 + GFF109 0.34 +2.1 95,649 + +0.3 95,896 + GFF110 0.20 -0.2 95,962 + GFF110 0.27 -1.9 96,104 - GFF110 0.41 +1.1 96,144 - GFF110 0.46 -0.3 96,144 - GFF110 0.46 -0.7 96,149 - GFF110 0.46 +0.8 96,215 + GFF110 0.53 -1.7 96,384 - GFF110 0.70 -0.8 96,683 - -0.9 96,683 - -2.9 96,697 + -0.4 96,720 - +0.6 97,399 + GFF111 0.57 -0.7 97,407 - GFF111 0.58 -0.7 97,410 + GFF111 0.58 -0.7 97,411 - GFF111 0.58 +0.5 97,419 + GFF111 0.59 -0.1 97,456 + GFF111 0.62 +0.3 97,486 - GFF111 0.64 -1.0 97,673 + GFF111 0.79 +0.5
Or see this region's nucleotide sequence