Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4234 and GFF4235 are separated by 155 nucleotides GFF4235 and GFF4236 overlap by 4 nucleotides GFF4236 and GFF4237 are separated by 323 nucleotides GFF4237 and GFF4238 are separated by 99 nucleotides GFF4238 and GFF4239 overlap by 28 nucleotides
GFF4234 - Possible transmembrane regulator, at 15,815 to 16,549
GFF4234
GFF4235 - Putative inner membrane protein, at 16,705 to 16,821
GFF4235
GFF4236 - Putative inner membrane protein, at 16,818 to 17,021
GFF4236
GFF4237 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 17,345 to 18,895
GFF4237
GFF4238 - Possible transmembrane regulator, at 18,995 to 19,738
GFF4238
GFF4239 - Putative inner membrane protein, at 19,711 to 20,199
GFF4239
Position (kb) 17
18
19 Strain fitness (log2 ratio) -1
0
1 at 16.787 kb on + strand, within GFF4235 at 17.254 kb on - strand at 17.465 kb on - strand at 17.570 kb on + strand, within GFF4237 at 17.570 kb on + strand, within GFF4237 at 17.747 kb on + strand, within GFF4237 at 17.747 kb on + strand, within GFF4237 at 17.747 kb on + strand, within GFF4237 at 17.748 kb on - strand, within GFF4237 at 17.802 kb on - strand, within GFF4237 at 17.802 kb on - strand, within GFF4237 at 18.141 kb on - strand, within GFF4237 at 18.271 kb on + strand, within GFF4237 at 18.377 kb on - strand, within GFF4237 at 18.391 kb on + strand, within GFF4237 at 18.393 kb on + strand, within GFF4237 at 18.665 kb on - strand, within GFF4237 at 18.670 kb on + strand, within GFF4237 at 18.671 kb on - strand, within GFF4237 at 18.738 kb on - strand, within GFF4237 at 18.744 kb on + strand at 18.745 kb on - strand at 18.808 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 16,787 + GFF4235 0.70 +0.1 17,254 - -0.6 17,465 - -0.8 17,570 + GFF4237 0.15 +0.1 17,570 + GFF4237 0.15 +0.5 17,747 + GFF4237 0.26 +0.8 17,747 + GFF4237 0.26 +0.4 17,747 + GFF4237 0.26 +0.2 17,748 - GFF4237 0.26 +0.1 17,802 - GFF4237 0.29 -0.2 17,802 - GFF4237 0.29 -0.6 18,141 - GFF4237 0.51 +0.8 18,271 + GFF4237 0.60 +0.2 18,377 - GFF4237 0.67 +0.8 18,391 + GFF4237 0.67 +0.3 18,393 + GFF4237 0.68 +0.1 18,665 - GFF4237 0.85 +0.2 18,670 + GFF4237 0.85 -0.1 18,671 - GFF4237 0.85 -0.1 18,738 - GFF4237 0.90 -0.6 18,744 + +0.0 18,745 - -0.1 18,808 + -0.1
Or see this region's nucleotide sequence