Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF409 and GFF410 overlap by 4 nucleotides GFF410 and GFF411 overlap by 4 nucleotides GFF411 and GFF412 are separated by 298 nucleotides
GFF409 - L-lactate dehydrogenase (EC 1.1.2.3), at 123,551 to 124,741
GFF409
GFF410 - Lactate-responsive regulator LldR in Enterobacteria, GntR family, at 124,738 to 125,514
GFF410
GFF411 - L-lactate permease, at 125,511 to 127,166
GFF411
GFF412 - Peptide transport system permease protein sapB (TC 3.A.1.5.5), at 127,465 to 131,850
GFF412
Position (kb) 125
126
127
128 Strain fitness (log2 ratio) -1
0
1 at 124.643 kb on - strand at 124.644 kb on + strand at 124.746 kb on + strand at 124.767 kb on + strand at 124.767 kb on + strand at 124.768 kb on - strand at 124.869 kb on + strand, within GFF410 at 124.958 kb on + strand, within GFF410 at 124.958 kb on + strand, within GFF410 at 124.959 kb on - strand, within GFF410 at 124.962 kb on + strand, within GFF410 at 124.962 kb on + strand, within GFF410 at 124.963 kb on - strand, within GFF410 at 124.963 kb on - strand, within GFF410 at 125.387 kb on - strand, within GFF410 at 125.549 kb on + strand at 125.567 kb on + strand at 125.567 kb on + strand at 125.568 kb on - strand at 125.568 kb on - strand at 125.757 kb on + strand, within GFF411 at 125.757 kb on + strand, within GFF411 at 125.897 kb on - strand, within GFF411 at 126.018 kb on - strand, within GFF411 at 126.076 kb on + strand, within GFF411 at 126.076 kb on + strand, within GFF411 at 126.077 kb on - strand, within GFF411 at 126.089 kb on - strand, within GFF411 at 126.159 kb on - strand, within GFF411 at 126.172 kb on + strand, within GFF411 at 126.173 kb on - strand, within GFF411 at 126.284 kb on + strand, within GFF411 at 126.285 kb on - strand, within GFF411 at 126.285 kb on - strand, within GFF411 at 126.285 kb on - strand, within GFF411 at 126.713 kb on + strand, within GFF411 at 126.956 kb on - strand, within GFF411 at 126.956 kb on - strand, within GFF411 at 127.048 kb on + strand at 127.048 kb on + strand at 127.049 kb on - strand at 127.049 kb on - strand at 127.049 kb on - strand at 127.049 kb on - strand at 127.068 kb on - strand at 127.233 kb on - strand at 127.286 kb on - strand at 127.317 kb on - strand at 127.346 kb on + strand at 127.458 kb on + strand at 127.466 kb on + strand at 127.533 kb on + strand at 127.558 kb on + strand at 127.788 kb on - strand at 127.804 kb on - strand at 127.877 kb on + strand at 127.877 kb on + strand at 127.878 kb on - strand at 128.028 kb on + strand, within GFF412 at 128.029 kb on - strand, within GFF412 at 128.031 kb on + strand, within GFF412 at 128.031 kb on + strand, within GFF412 at 128.060 kb on - strand, within GFF412 at 128.089 kb on - strand, within GFF412 at 128.163 kb on - strand, within GFF412
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 124,643 - -0.5 124,644 + +0.0 124,746 + +0.8 124,767 + +0.2 124,767 + -0.4 124,768 - +0.0 124,869 + GFF410 0.17 -0.6 124,958 + GFF410 0.28 +0.6 124,958 + GFF410 0.28 +0.1 124,959 - GFF410 0.28 +0.8 124,962 + GFF410 0.29 -0.5 124,962 + GFF410 0.29 +0.1 124,963 - GFF410 0.29 +0.2 124,963 - GFF410 0.29 +0.5 125,387 - GFF410 0.84 +1.0 125,549 + +0.0 125,567 + +0.2 125,567 + -0.1 125,568 - -0.4 125,568 - -0.8 125,757 + GFF411 0.15 -0.4 125,757 + GFF411 0.15 +0.1 125,897 - GFF411 0.23 +0.0 126,018 - GFF411 0.31 -0.1 126,076 + GFF411 0.34 +0.2 126,076 + GFF411 0.34 -0.2 126,077 - GFF411 0.34 +0.3 126,089 - GFF411 0.35 +0.4 126,159 - GFF411 0.39 -0.9 126,172 + GFF411 0.40 +0.7 126,173 - GFF411 0.40 +0.0 126,284 + GFF411 0.47 -0.1 126,285 - GFF411 0.47 -0.3 126,285 - GFF411 0.47 +0.7 126,285 - GFF411 0.47 +0.5 126,713 + GFF411 0.73 +0.1 126,956 - GFF411 0.87 -0.2 126,956 - GFF411 0.87 +0.2 127,048 + +0.9 127,048 + +1.0 127,049 - -0.3 127,049 - +0.2 127,049 - +1.2 127,049 - -0.3 127,068 - +0.2 127,233 - -1.3 127,286 - -0.4 127,317 - +0.0 127,346 + -0.2 127,458 + -0.0 127,466 + -0.2 127,533 + +0.0 127,558 + +0.2 127,788 - -0.2 127,804 - -0.7 127,877 + +0.6 127,877 + -0.3 127,878 - +0.2 128,028 + GFF412 0.13 -0.6 128,029 - GFF412 0.13 -1.2 128,031 + GFF412 0.13 -0.1 128,031 + GFF412 0.13 +0.4 128,060 - GFF412 0.14 -0.3 128,089 - GFF412 0.14 -0.3 128,163 - GFF412 0.16 +0.1
Or see this region's nucleotide sequence