Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3921 and GFF3922 overlap by 4 nucleotides GFF3922 and GFF3923 are separated by 123 nucleotides GFF3923 and GFF3924 are separated by 152 nucleotides GFF3924 and GFF3925 are separated by 118 nucleotides
GFF3921 - Thiosulfate reductase electron transport protein phsB, at 206,193 to 206,771
GFF3921
GFF3922 - Thiosulfate reductase cytochrome B subunit, at 206,768 to 207,532
GFF3922
GFF3923 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 207,656 to 208,828
GFF3923
GFF3924 - DNA gyrase inhibitory protein, at 208,981 to 209,448
GFF3924
GFF3925 - Putative inner membrane protein, at 209,567 to 210,625
GFF3925
Position (kb) 207
208
209 Strain fitness (log2 ratio) -2
-1
0
1 at 206.763 kb on - strand at 206.774 kb on - strand at 206.776 kb on + strand at 206.793 kb on + strand at 206.793 kb on + strand at 206.937 kb on - strand, within GFF3922 at 206.998 kb on - strand, within GFF3922 at 207.051 kb on + strand, within GFF3922 at 207.250 kb on + strand, within GFF3922 at 207.279 kb on - strand, within GFF3922 at 207.294 kb on - strand, within GFF3922 at 207.294 kb on - strand, within GFF3922 at 207.853 kb on + strand, within GFF3923 at 207.853 kb on + strand, within GFF3923 at 207.862 kb on - strand, within GFF3923 at 207.990 kb on + strand, within GFF3923 at 208.125 kb on - strand, within GFF3923 at 208.125 kb on - strand, within GFF3923 at 208.195 kb on + strand, within GFF3923 at 208.195 kb on + strand, within GFF3923 at 208.266 kb on - strand, within GFF3923 at 208.266 kb on - strand, within GFF3923 at 208.331 kb on - strand, within GFF3923 at 208.354 kb on + strand, within GFF3923 at 208.652 kb on + strand, within GFF3923 at 208.664 kb on - strand, within GFF3923 at 208.786 kb on + strand at 208.827 kb on - strand at 209.018 kb on + strand at 209.153 kb on + strand, within GFF3924 at 209.153 kb on + strand, within GFF3924 at 209.348 kb on - strand, within GFF3924 at 209.354 kb on + strand, within GFF3924 at 209.427 kb on + strand at 209.519 kb on + strand at 209.520 kb on - strand at 209.529 kb on - strand at 209.529 kb on - strand at 209.586 kb on + strand at 209.609 kb on + strand at 209.610 kb on - strand at 209.642 kb on - strand at 209.722 kb on - strand, within GFF3925 at 209.731 kb on + strand, within GFF3925
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 206,763 - -0.6 206,774 - -0.1 206,776 + +0.5 206,793 + -0.7 206,793 + +0.7 206,937 - GFF3922 0.22 -1.2 206,998 - GFF3922 0.30 +0.7 207,051 + GFF3922 0.37 +0.1 207,250 + GFF3922 0.63 -0.6 207,279 - GFF3922 0.67 -0.7 207,294 - GFF3922 0.69 -0.3 207,294 - GFF3922 0.69 +0.1 207,853 + GFF3923 0.17 +0.2 207,853 + GFF3923 0.17 +0.6 207,862 - GFF3923 0.18 +0.2 207,990 + GFF3923 0.28 -0.2 208,125 - GFF3923 0.40 -1.9 208,125 - GFF3923 0.40 -0.1 208,195 + GFF3923 0.46 -0.7 208,195 + GFF3923 0.46 +0.5 208,266 - GFF3923 0.52 +0.2 208,266 - GFF3923 0.52 -0.4 208,331 - GFF3923 0.58 -0.5 208,354 + GFF3923 0.60 -0.4 208,652 + GFF3923 0.85 -0.5 208,664 - GFF3923 0.86 -1.1 208,786 + +0.7 208,827 - +0.1 209,018 + -0.2 209,153 + GFF3924 0.37 -1.0 209,153 + GFF3924 0.37 -1.3 209,348 - GFF3924 0.78 +0.6 209,354 + GFF3924 0.80 +0.2 209,427 + +0.1 209,519 + -0.4 209,520 - -0.6 209,529 - -0.8 209,529 - +0.5 209,586 + +0.3 209,609 + -0.7 209,610 - -1.3 209,642 - -0.1 209,722 - GFF3925 0.15 +0.5 209,731 + GFF3925 0.15 +0.3
Or see this region's nucleotide sequence