Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF3352

Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3349 and GFF3350 are separated by 81 nucleotidesGFF3350 and GFF3351 are separated by 34 nucleotidesGFF3351 and GFF3352 are separated by 0 nucleotidesGFF3352 and GFF3353 overlap by 26 nucleotidesGFF3353 and GFF3354 are separated by 156 nucleotides GFF3349 - Proposed peptidoglycan lipid II flippase MurJ, at 117,015 to 118,589 GFF3349 GFF3350 - Flagellar biosynthesis protein FlgN, at 118,671 to 119,063 GFF3350 GFF3351 - Negative regulator of flagellin synthesis FlgM, at 119,098 to 119,391 GFF3351 GFF3352 - hypothetical protein, at 119,392 to 119,508 GFF3352 GFF3353 - Flagellar basal-body P-ring formation protein FlgA, at 119,483 to 120,142 GFF3353 GFF3354 - Flagellar basal-body rod protein FlgB, at 120,299 to 120,715 GFF3354 Position (kb) 119 120Strain fitness (log2 ratio) -2 -1 0 1 2at 118.624 kb on - strandat 118.729 kb on + strand, within GFF3350at 118.729 kb on + strand, within GFF3350at 118.967 kb on + strand, within GFF3350at 118.971 kb on - strand, within GFF3350at 118.977 kb on + strand, within GFF3350at 119.094 kb on - strandat 119.100 kb on - strandat 119.100 kb on - strandat 119.104 kb on - strandat 119.104 kb on - strandat 119.115 kb on - strandat 119.215 kb on - strand, within GFF3351at 119.582 kb on - strand, within GFF3353at 119.639 kb on + strand, within GFF3353at 119.675 kb on + strand, within GFF3353at 119.676 kb on - strand, within GFF3353at 119.683 kb on + strand, within GFF3353at 120.146 kb on - strandat 120.179 kb on - strandat 120.207 kb on - strandat 120.210 kb on + strandat 120.211 kb on - strandat 120.261 kb on + strandat 120.262 kb on - strandat 120.298 kb on + strandat 120.401 kb on - strand, within GFF3354

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI
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118,624 - +0.3
118,729 + GFF3350 0.15 -0.2
118,729 + GFF3350 0.15 -1.8
118,967 + GFF3350 0.75 +2.5
118,971 - GFF3350 0.76 +0.1
118,977 + GFF3350 0.78 -0.0
119,094 - -0.4
119,100 - +0.2
119,100 - -0.2
119,104 - +0.2
119,104 - -0.5
119,115 - +0.3
119,215 - GFF3351 0.40 -0.3
119,582 - GFF3353 0.15 -0.1
119,639 + GFF3353 0.24 -0.3
119,675 + GFF3353 0.29 -0.7
119,676 - GFF3353 0.29 -0.4
119,683 + GFF3353 0.30 +0.0
120,146 - +0.2
120,179 - +0.5
120,207 - +0.6
120,210 + -0.8
120,211 - -0.4
120,261 + -1.4
120,262 - +0.1
120,298 + -0.8
120,401 - GFF3354 0.24 -0.8

Or see this region's nucleotide sequence