Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3327 and GFF3328 are separated by 253 nucleotides GFF3328 and GFF3329 overlap by 8 nucleotides
GFF3327 - Glucans biosynthesis protein C (EC 2.1.-.-), at 97,043 to 98,197
GFF3327
GFF3328 - Glucans biosynthesis protein G precursor, at 98,451 to 100,004
GFF3328
GFF3329 - Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-), at 99,997 to 102,540
GFF3329
Position (kb) 98
99
100
101 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 97.599 kb on - strand, within GFF3327 at 97.630 kb on - strand, within GFF3327 at 97.726 kb on + strand, within GFF3327 at 97.727 kb on - strand, within GFF3327 at 98.104 kb on - strand at 98.132 kb on + strand at 98.155 kb on + strand at 98.197 kb on + strand at 98.199 kb on + strand at 98.200 kb on - strand at 98.209 kb on + strand at 98.270 kb on - strand at 98.418 kb on - strand at 98.443 kb on + strand at 98.444 kb on - strand at 98.444 kb on - strand at 98.448 kb on - strand at 98.459 kb on + strand at 98.459 kb on + strand at 98.502 kb on - strand at 98.689 kb on + strand, within GFF3328 at 98.689 kb on + strand, within GFF3328 at 98.690 kb on - strand, within GFF3328 at 98.703 kb on - strand, within GFF3328 at 98.730 kb on + strand, within GFF3328 at 98.803 kb on - strand, within GFF3328 at 98.918 kb on + strand, within GFF3328 at 98.934 kb on + strand, within GFF3328 at 98.934 kb on + strand, within GFF3328 at 98.965 kb on - strand, within GFF3328 at 98.972 kb on - strand, within GFF3328 at 99.224 kb on + strand, within GFF3328 at 99.298 kb on + strand, within GFF3328 at 99.372 kb on + strand, within GFF3328 at 99.373 kb on - strand, within GFF3328 at 99.453 kb on + strand, within GFF3328 at 99.551 kb on + strand, within GFF3328 at 99.848 kb on + strand, within GFF3328 at 99.853 kb on + strand at 99.853 kb on + strand at 99.854 kb on - strand at 100.115 kb on - strand at 100.200 kb on - strand at 100.200 kb on - strand at 100.403 kb on + strand, within GFF3329 at 100.404 kb on - strand, within GFF3329 at 100.404 kb on - strand, within GFF3329 at 100.404 kb on - strand, within GFF3329 at 100.459 kb on + strand, within GFF3329 at 100.460 kb on - strand, within GFF3329 at 100.461 kb on + strand, within GFF3329 at 100.462 kb on - strand, within GFF3329 at 100.506 kb on + strand, within GFF3329 at 100.507 kb on - strand, within GFF3329 at 100.527 kb on + strand, within GFF3329 at 100.690 kb on - strand, within GFF3329 at 100.837 kb on + strand, within GFF3329 at 100.838 kb on - strand, within GFF3329 at 100.856 kb on - strand, within GFF3329
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 97,599 - GFF3327 0.48 -0.0 97,630 - GFF3327 0.51 -0.1 97,726 + GFF3327 0.59 -0.5 97,727 - GFF3327 0.59 +0.5 98,104 - -0.3 98,132 + -0.8 98,155 + -0.0 98,197 + +0.3 98,199 + +0.5 98,200 - -0.2 98,209 + -0.5 98,270 - +1.8 98,418 - -1.5 98,443 + +0.0 98,444 - -0.1 98,444 - -0.8 98,448 - -0.8 98,459 + -0.1 98,459 + -2.4 98,502 - -0.5 98,689 + GFF3328 0.15 -1.0 98,689 + GFF3328 0.15 +0.4 98,690 - GFF3328 0.15 -0.7 98,703 - GFF3328 0.16 -1.0 98,730 + GFF3328 0.18 -1.0 98,803 - GFF3328 0.23 -1.1 98,918 + GFF3328 0.30 -0.5 98,934 + GFF3328 0.31 -0.2 98,934 + GFF3328 0.31 -0.9 98,965 - GFF3328 0.33 -1.0 98,972 - GFF3328 0.34 -1.6 99,224 + GFF3328 0.50 -1.1 99,298 + GFF3328 0.55 -0.9 99,372 + GFF3328 0.59 -0.3 99,373 - GFF3328 0.59 -1.1 99,453 + GFF3328 0.64 -0.1 99,551 + GFF3328 0.71 +0.0 99,848 + GFF3328 0.90 -0.3 99,853 + -1.1 99,853 + -0.6 99,854 - -1.0 100,115 - -0.7 100,200 - +1.2 100,200 - -2.2 100,403 + GFF3329 0.16 -0.6 100,404 - GFF3329 0.16 +0.7 100,404 - GFF3329 0.16 -3.1 100,404 - GFF3329 0.16 -1.7 100,459 + GFF3329 0.18 -0.7 100,460 - GFF3329 0.18 -0.8 100,461 + GFF3329 0.18 +0.3 100,462 - GFF3329 0.18 -1.3 100,506 + GFF3329 0.20 -0.7 100,507 - GFF3329 0.20 -1.4 100,527 + GFF3329 0.21 -0.1 100,690 - GFF3329 0.27 -1.1 100,837 + GFF3329 0.33 -1.1 100,838 - GFF3329 0.33 -0.7 100,856 - GFF3329 0.34 -2.1
Or see this region's nucleotide sequence