Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3266 and GFF3267 are separated by 547 nucleotides GFF3267 and GFF3268 are separated by 16 nucleotides GFF3268 and GFF3269 are separated by 361 nucleotides GFF3269 and GFF3270 are separated by 31 nucleotides GFF3270 and GFF3271 are separated by 2 nucleotides
GFF3266 - FIG01046201: hypothetical protein, at 38,316 to 39,191
GFF3266
GFF3267 - Invasion gene E protein (Pathogenicity island encoded protein: homologous to ipgE of Shigella), at 39,739 to 40,080
GFF3267
GFF3268 - Inositol phosphate phosphatase sopB (EC 3.1.3.-), at 40,097 to 41,782
GFF3268
GFF3269 - FIG01045843: hypothetical protein, at 42,144 to 42,284
GFF3269
GFF3270 - FIG01046223: hypothetical protein, at 42,316 to 42,501
GFF3270
GFF3271 - Probable dipeptidase (EC 3.4.-.-), at 42,504 to 44,066
GFF3271
Position (kb) 40
41
42 Strain fitness (log2 ratio) -1
0
1 at 39.253 kb on - strand at 39.852 kb on + strand, within GFF3267 at 40.082 kb on + strand at 40.141 kb on + strand at 40.328 kb on - strand, within GFF3268 at 40.328 kb on - strand, within GFF3268 at 40.651 kb on - strand, within GFF3268 at 40.651 kb on - strand, within GFF3268 at 40.712 kb on - strand, within GFF3268 at 40.712 kb on - strand, within GFF3268 at 41.229 kb on + strand, within GFF3268 at 41.380 kb on + strand, within GFF3268 at 41.546 kb on + strand, within GFF3268 at 41.553 kb on + strand, within GFF3268 at 41.554 kb on - strand, within GFF3268 at 41.560 kb on + strand, within GFF3268 at 41.635 kb on + strand at 41.783 kb on - strand at 41.944 kb on - strand at 41.944 kb on - strand at 42.064 kb on - strand at 42.275 kb on + strand at 42.294 kb on + strand at 42.336 kb on - strand, within GFF3270 at 42.336 kb on - strand, within GFF3270 at 42.340 kb on - strand, within GFF3270 at 42.705 kb on - strand, within GFF3271 at 42.764 kb on - strand, within GFF3271
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 39,253 - +0.0 39,852 + GFF3267 0.33 -1.0 40,082 + +0.6 40,141 + +0.5 40,328 - GFF3268 0.14 +0.4 40,328 - GFF3268 0.14 +0.5 40,651 - GFF3268 0.33 +0.4 40,651 - GFF3268 0.33 -0.4 40,712 - GFF3268 0.36 +0.7 40,712 - GFF3268 0.36 +0.9 41,229 + GFF3268 0.67 +0.0 41,380 + GFF3268 0.76 +0.0 41,546 + GFF3268 0.86 +1.1 41,553 + GFF3268 0.86 +0.3 41,554 - GFF3268 0.86 +0.3 41,560 + GFF3268 0.87 +0.4 41,635 + -0.4 41,783 - +0.6 41,944 - -0.5 41,944 - +0.5 42,064 - -0.2 42,275 + -0.1 42,294 + +0.2 42,336 - GFF3270 0.11 -0.4 42,336 - GFF3270 0.11 +0.2 42,340 - GFF3270 0.13 -0.2 42,705 - GFF3271 0.13 -0.3 42,764 - GFF3271 0.17 +0.2
Or see this region's nucleotide sequence