Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3081 and GFF3082 overlap by 8 nucleotides GFF3082 and GFF3083 are separated by 72 nucleotides GFF3083 and GFF3084 are separated by 14 nucleotides GFF3084 and GFF3085 are separated by 161 nucleotides
GFF3081 - Unknown, probable amino acid transporter, at 90,547 to 92,046
GFF3081
GFF3082 - hypothetical protein, at 92,039 to 92,182
GFF3082
GFF3083 - FIG074102: hypothetical protein, at 92,255 to 92,629
GFF3083
GFF3084 - FIG00553873: hypothetical protein, at 92,644 to 93,945
GFF3084
GFF3085 - Aldehyde dehydrogenase (EC 1.2.1.3), at 94,107 to 95,591
GFF3085
Position (kb)
92
93
94 Strain fitness (log2 ratio)
-1
0
1 at 91.800 kb on + strand, within GFF3081 at 91.831 kb on - strand, within GFF3081 at 91.878 kb on + strand, within GFF3081 at 91.878 kb on + strand, within GFF3081 at 91.891 kb on + strand, within GFF3081 at 92.041 kb on - strand at 92.076 kb on + strand, within GFF3082 at 92.101 kb on + strand, within GFF3082 at 92.101 kb on + strand, within GFF3082 at 92.101 kb on + strand, within GFF3082 at 92.102 kb on - strand, within GFF3082 at 92.105 kb on + strand, within GFF3082 at 92.120 kb on - strand, within GFF3082 at 92.123 kb on + strand, within GFF3082 at 92.123 kb on + strand, within GFF3082 at 92.124 kb on - strand, within GFF3082 at 92.124 kb on - strand, within GFF3082 at 92.124 kb on - strand, within GFF3082 at 92.210 kb on - strand at 92.492 kb on - strand, within GFF3083 at 92.495 kb on + strand, within GFF3083 at 92.495 kb on + strand, within GFF3083 at 92.495 kb on + strand, within GFF3083 at 92.496 kb on - strand, within GFF3083 at 92.630 kb on + strand at 92.630 kb on + strand at 92.630 kb on + strand at 92.631 kb on - strand at 92.707 kb on + strand at 92.708 kb on - strand at 92.786 kb on - strand, within GFF3084 at 92.810 kb on - strand, within GFF3084 at 92.960 kb on + strand, within GFF3084 at 93.015 kb on + strand, within GFF3084 at 93.160 kb on - strand, within GFF3084 at 93.259 kb on + strand, within GFF3084 at 93.600 kb on + strand, within GFF3084 at 93.673 kb on + strand, within GFF3084 at 93.673 kb on + strand, within GFF3084 at 93.674 kb on - strand, within GFF3084 at 93.706 kb on + strand, within GFF3084 at 93.707 kb on - strand, within GFF3084 at 93.783 kb on + strand, within GFF3084 at 93.918 kb on - strand at 93.981 kb on + strand at 94.060 kb on + strand at 94.060 kb on + strand at 94.061 kb on - strand at 94.080 kb on + strand at 94.123 kb on + strand at 94.169 kb on + strand at 94.368 kb on - strand, within GFF3085 at 94.368 kb on - strand, within GFF3085 at 94.431 kb on - strand, within GFF3085 at 94.622 kb on + strand, within GFF3085 at 94.622 kb on + strand, within GFF3085 at 94.623 kb on - strand, within GFF3085 at 94.657 kb on + strand, within GFF3085 at 94.680 kb on - strand, within GFF3085 at 94.692 kb on + strand, within GFF3085 at 94.692 kb on + strand, within GFF3085 at 94.693 kb on - strand, within GFF3085 at 94.693 kb on - strand, within GFF3085 at 94.694 kb on + strand, within GFF3085 at 94.695 kb on - strand, within GFF3085 at 94.769 kb on - strand, within GFF3085 at 94.909 kb on + strand, within GFF3085 at 94.910 kb on - strand, within GFF3085
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 91,800 + GFF3081 0.84 +0.4 91,831 - GFF3081 0.86 -0.7 91,878 + GFF3081 0.89 +0.1 91,878 + GFF3081 0.89 +0.8 91,891 + GFF3081 0.90 -0.2 92,041 - +0.2 92,076 + GFF3082 0.26 +0.4 92,101 + GFF3082 0.43 +0.7 92,101 + GFF3082 0.43 -0.2 92,101 + GFF3082 0.43 -0.5 92,102 - GFF3082 0.44 +0.0 92,105 + GFF3082 0.46 -0.1 92,120 - GFF3082 0.56 -0.5 92,123 + GFF3082 0.58 -0.7 92,123 + GFF3082 0.58 +0.0 92,124 - GFF3082 0.59 +0.4 92,124 - GFF3082 0.59 +0.1 92,124 - GFF3082 0.59 -0.1 92,210 - +0.6 92,492 - GFF3083 0.63 +0.3 92,495 + GFF3083 0.64 +0.5 92,495 + GFF3083 0.64 +0.4 92,495 + GFF3083 0.64 -0.5 92,496 - GFF3083 0.64 -0.0 92,630 + -0.2 92,630 + +0.4 92,630 + -0.3 92,631 - -0.2 92,707 + -0.6 92,708 - -0.8 92,786 - GFF3084 0.11 +0.7 92,810 - GFF3084 0.13 +1.0 92,960 + GFF3084 0.24 -1.1 93,015 + GFF3084 0.28 +0.6 93,160 - GFF3084 0.40 +0.7 93,259 + GFF3084 0.47 +0.1 93,600 + GFF3084 0.73 +0.2 93,673 + GFF3084 0.79 -0.1 93,673 + GFF3084 0.79 -0.3 93,674 - GFF3084 0.79 +0.4 93,706 + GFF3084 0.82 +0.5 93,707 - GFF3084 0.82 +0.2 93,783 + GFF3084 0.87 -0.1 93,918 - +0.6 93,981 + -0.0 94,060 + -0.1 94,060 + +1.4 94,061 - +1.3 94,080 + -0.2 94,123 + +0.3 94,169 + +0.4 94,368 - GFF3085 0.18 -0.1 94,368 - GFF3085 0.18 -0.2 94,431 - GFF3085 0.22 -0.1 94,622 + GFF3085 0.35 -0.2 94,622 + GFF3085 0.35 +0.8 94,623 - GFF3085 0.35 +0.0 94,657 + GFF3085 0.37 +0.4 94,680 - GFF3085 0.39 -0.2 94,692 + GFF3085 0.39 -0.5 94,692 + GFF3085 0.39 -0.4 94,693 - GFF3085 0.39 +0.1 94,693 - GFF3085 0.39 -0.4 94,694 + GFF3085 0.40 -0.2 94,695 - GFF3085 0.40 +0.1 94,769 - GFF3085 0.45 -0.1 94,909 + GFF3085 0.54 -0.3 94,910 - GFF3085 0.54 +0.3
Or see this region's nucleotide sequence