Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2173 and GFF2174 are separated by 26 nucleotides GFF2174 and GFF2175 are separated by 39 nucleotides GFF2175 and GFF2176 are separated by 216 nucleotides
GFF2173 - Chromosomal replication initiator protein DnaA, at 20,799 to 21,524
GFF2173
GFF2174 - Arsenate reductase (EC 1.20.4.1), at 21,551 to 21,910
GFF2174
GFF2175 - Exported zinc metalloprotease YfgC precursor, at 21,950 to 23,413
GFF2175
GFF2176 - Putative permease PerM (= YfgO), at 23,630 to 24,688
GFF2176
Position (kb) 21
22
23
24 Strain fitness (log2 ratio) -1
0
1 at 20.955 kb on + strand, within GFF2173 at 20.955 kb on + strand, within GFF2173 at 20.955 kb on + strand, within GFF2173 at 20.955 kb on + strand, within GFF2173 at 20.956 kb on - strand, within GFF2173 at 20.957 kb on + strand, within GFF2173 at 20.957 kb on + strand, within GFF2173 at 20.961 kb on + strand, within GFF2173 at 21.183 kb on + strand, within GFF2173 at 21.251 kb on - strand, within GFF2173 at 21.760 kb on + strand, within GFF2174 at 21.761 kb on - strand, within GFF2174 at 21.889 kb on - strand at 21.964 kb on - strand at 21.984 kb on - strand at 22.104 kb on + strand, within GFF2175 at 22.209 kb on + strand, within GFF2175 at 22.275 kb on - strand, within GFF2175 at 22.546 kb on + strand, within GFF2175 at 22.561 kb on - strand, within GFF2175 at 22.561 kb on - strand, within GFF2175 at 23.190 kb on + strand, within GFF2175 at 23.191 kb on - strand, within GFF2175 at 23.234 kb on + strand, within GFF2175 at 23.463 kb on + strand at 23.464 kb on - strand at 23.489 kb on - strand at 23.495 kb on - strand at 23.523 kb on + strand at 23.524 kb on - strand at 23.737 kb on - strand, within GFF2176 at 23.913 kb on - strand, within GFF2176 at 24.047 kb on - strand, within GFF2176 at 24.113 kb on + strand, within GFF2176 at 24.150 kb on + strand, within GFF2176 at 24.254 kb on + strand, within GFF2176 at 24.254 kb on + strand, within GFF2176 at 24.254 kb on + strand, within GFF2176 at 24.257 kb on - strand, within GFF2176 at 24.332 kb on + strand, within GFF2176 at 24.332 kb on + strand, within GFF2176 at 24.381 kb on - strand, within GFF2176
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 20,955 + GFF2173 0.21 +0.8 20,955 + GFF2173 0.21 +0.2 20,955 + GFF2173 0.21 +0.6 20,955 + GFF2173 0.21 +1.0 20,956 - GFF2173 0.22 +1.1 20,957 + GFF2173 0.22 +0.4 20,957 + GFF2173 0.22 -0.1 20,961 + GFF2173 0.22 +1.3 21,183 + GFF2173 0.53 +0.5 21,251 - GFF2173 0.62 +1.5 21,760 + GFF2174 0.58 -0.2 21,761 - GFF2174 0.58 +0.4 21,889 - +0.3 21,964 - -0.1 21,984 - +0.5 22,104 + GFF2175 0.11 +0.3 22,209 + GFF2175 0.18 +0.1 22,275 - GFF2175 0.22 -0.0 22,546 + GFF2175 0.41 +0.4 22,561 - GFF2175 0.42 +0.1 22,561 - GFF2175 0.42 -0.3 23,190 + GFF2175 0.85 -0.3 23,191 - GFF2175 0.85 -0.8 23,234 + GFF2175 0.88 -0.7 23,463 + -0.5 23,464 - +0.1 23,489 - -0.4 23,495 - +0.5 23,523 + +0.7 23,524 - -0.1 23,737 - GFF2176 0.10 +0.6 23,913 - GFF2176 0.27 +0.3 24,047 - GFF2176 0.39 -0.2 24,113 + GFF2176 0.46 -1.0 24,150 + GFF2176 0.49 +0.6 24,254 + GFF2176 0.59 +0.7 24,254 + GFF2176 0.59 -0.1 24,254 + GFF2176 0.59 +0.6 24,257 - GFF2176 0.59 +0.0 24,332 + GFF2176 0.66 +0.0 24,332 + GFF2176 0.66 -0.5 24,381 - GFF2176 0.71 -0.3
Or see this region's nucleotide sequence