Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1996 and GFF1997 overlap by 8 nucleotides GFF1997 and GFF1998 are separated by 10 nucleotides GFF1998 and GFF1999 are separated by 10 nucleotides GFF1999 and GFF2000 are separated by 111 nucleotides
GFF1996 - Succinate dehydrogenase flavoprotein subunit (EC 1.3.99.1), at 10,702 to 12,492
GFF1996
GFF1997 - Succinate dehydrogenase iron-sulfur protein (EC 1.3.99.1), at 12,485 to 13,219
GFF1997
GFF1998 - Fumarate reductase subunit C, at 13,230 to 13,625
GFF1998
GFF1999 - Fumarate reductase subunit D, at 13,636 to 13,995
GFF1999
GFF2000 - Outer membrane lipoprotein Blc, at 14,107 to 14,640
GFF2000
Position (kb) 13
14 Strain fitness (log2 ratio) -2
-1
0
1 at 12.255 kb on + strand, within GFF1996 at 12.255 kb on + strand, within GFF1996 at 12.469 kb on - strand at 12.611 kb on + strand, within GFF1997 at 12.611 kb on + strand, within GFF1997 at 12.738 kb on - strand, within GFF1997 at 12.842 kb on + strand, within GFF1997 at 12.842 kb on + strand, within GFF1997 at 12.847 kb on + strand, within GFF1997 at 12.847 kb on + strand, within GFF1997 at 12.951 kb on - strand, within GFF1997 at 13.238 kb on + strand at 13.251 kb on + strand at 13.251 kb on + strand at 13.252 kb on - strand at 13.252 kb on - strand at 13.305 kb on + strand, within GFF1998 at 13.306 kb on - strand, within GFF1998 at 13.307 kb on + strand, within GFF1998 at 13.308 kb on - strand, within GFF1998 at 13.594 kb on + strand at 13.606 kb on - strand at 13.737 kb on - strand, within GFF1999 at 13.767 kb on - strand, within GFF1999 at 13.874 kb on + strand, within GFF1999 at 13.939 kb on + strand, within GFF1999 at 13.993 kb on + strand at 14.014 kb on + strand at 14.079 kb on + strand at 14.164 kb on - strand, within GFF2000 at 14.199 kb on - strand, within GFF2000 at 14.237 kb on - strand, within GFF2000 at 14.296 kb on + strand, within GFF2000 at 14.296 kb on + strand, within GFF2000 at 14.455 kb on - strand, within GFF2000 at 14.613 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 12,255 + GFF1996 0.87 -0.2 12,255 + GFF1996 0.87 -0.9 12,469 - -0.6 12,611 + GFF1997 0.17 +0.4 12,611 + GFF1997 0.17 -0.9 12,738 - GFF1997 0.34 +1.3 12,842 + GFF1997 0.49 +0.3 12,842 + GFF1997 0.49 -0.7 12,847 + GFF1997 0.49 -0.5 12,847 + GFF1997 0.49 -0.5 12,951 - GFF1997 0.63 +0.3 13,238 + -0.1 13,251 + -0.3 13,251 + -0.6 13,252 - -0.1 13,252 - +0.1 13,305 + GFF1998 0.19 +0.3 13,306 - GFF1998 0.19 -0.5 13,307 + GFF1998 0.19 -1.1 13,308 - GFF1998 0.20 -0.4 13,594 + -1.0 13,606 - -0.2 13,737 - GFF1999 0.28 -0.3 13,767 - GFF1999 0.36 -0.8 13,874 + GFF1999 0.66 -0.1 13,939 + GFF1999 0.84 +0.1 13,993 + +0.5 14,014 + +0.2 14,079 + -0.0 14,164 - GFF2000 0.11 +0.1 14,199 - GFF2000 0.17 +0.6 14,237 - GFF2000 0.24 -1.2 14,296 + GFF2000 0.35 -2.5 14,296 + GFF2000 0.35 -0.1 14,455 - GFF2000 0.65 +0.3 14,613 - -0.8
Or see this region's nucleotide sequence