Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1808 and GFF1809 are separated by 102 nucleotides GFF1809 and GFF1810 are separated by 188 nucleotides GFF1810 and GFF1811 are separated by 365 nucleotides
GFF1808 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 31,798 to 32,973
GFF1808
GFF1809 - Putative GTP-binding protein YdgA, at 33,076 to 34,584
GFF1809
GFF1810 - FIG01046382: hypothetical protein, at 34,773 to 34,922
GFF1810
GFF1811 - Adenosine deaminase (EC 3.5.4.4), at 35,288 to 36,289
GFF1811
Position (kb)
33
34
35 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 32.174 kb on - strand, within GFF1808 at 32.174 kb on - strand, within GFF1808 at 32.566 kb on + strand, within GFF1808 at 32.683 kb on - strand, within GFF1808 at 32.683 kb on - strand, within GFF1808 at 32.856 kb on + strand at 32.856 kb on + strand at 32.980 kb on - strand at 33.011 kb on - strand at 33.092 kb on + strand at 33.108 kb on - strand at 33.319 kb on - strand, within GFF1809 at 33.418 kb on - strand, within GFF1809 at 33.501 kb on + strand, within GFF1809 at 33.520 kb on + strand, within GFF1809 at 33.718 kb on - strand, within GFF1809 at 34.447 kb on - strand at 34.447 kb on - strand at 34.469 kb on - strand at 34.644 kb on - strand at 34.825 kb on + strand, within GFF1810 at 34.852 kb on + strand, within GFF1810 at 34.852 kb on + strand, within GFF1810 at 34.920 kb on + strand at 34.980 kb on + strand at 35.031 kb on - strand at 35.102 kb on - strand at 35.247 kb on - strand at 35.251 kb on + strand at 35.296 kb on + strand at 35.299 kb on + strand at 35.299 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 32,174 - GFF1808 0.32 +1.4 32,174 - GFF1808 0.32 -0.3 32,566 + GFF1808 0.65 +0.3 32,683 - GFF1808 0.75 -1.4 32,683 - GFF1808 0.75 -1.3 32,856 + -0.4 32,856 + -0.1 32,980 - -0.3 33,011 - +0.1 33,092 + +1.4 33,108 - -0.6 33,319 - GFF1809 0.16 +0.4 33,418 - GFF1809 0.23 +0.5 33,501 + GFF1809 0.28 +0.4 33,520 + GFF1809 0.29 +0.4 33,718 - GFF1809 0.43 +0.3 34,447 - +0.3 34,447 - -3.2 34,469 - +0.4 34,644 - -1.5 34,825 + GFF1810 0.35 +0.2 34,852 + GFF1810 0.53 -0.0 34,852 + GFF1810 0.53 -0.0 34,920 + -0.3 34,980 + +0.2 35,031 - +0.7 35,102 - -0.5 35,247 - -0.4 35,251 + -0.2 35,296 + -0.4 35,299 + +0.9 35,299 + +1.7
Or see this region's nucleotide sequence