Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1738 and GFF1739 are separated by 94 nucleotides GFF1739 and GFF1740 are separated by 168 nucleotides GFF1740 and GFF1741 are separated by 44 nucleotides GFF1741 and GFF1742 overlap by 1 nucleotides
GFF1738 - Phosphate transport system regulatory protein PhoU, at 39,114 to 39,839
GFF1738
GFF1739 - Putative phosphatase YieH, at 39,934 to 40,599
GFF1739
GFF1740 - Xanthine/uracil/thiamine/ascorbate permease family protein, at 40,768 to 42,105
GFF1740
GFF1741 - FIG00553873: hypothetical protein, at 42,150 to 42,734
GFF1741
GFF1742 - FIG004212: hypothetical protein, at 42,734 to 43,489
GFF1742
Position (kb) 40
41
42
43 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 39.806 kb on + strand at 39.880 kb on + strand at 39.886 kb on + strand at 39.935 kb on + strand at 39.942 kb on + strand at 39.993 kb on + strand at 40.029 kb on + strand, within GFF1739 at 40.239 kb on + strand, within GFF1739 at 40.300 kb on - strand, within GFF1739 at 40.300 kb on - strand, within GFF1739 at 40.300 kb on - strand, within GFF1739 at 40.454 kb on - strand, within GFF1739 at 40.454 kb on - strand, within GFF1739 at 40.474 kb on + strand, within GFF1739 at 40.474 kb on + strand, within GFF1739 at 40.475 kb on - strand, within GFF1739 at 40.494 kb on + strand, within GFF1739 at 40.495 kb on - strand, within GFF1739 at 40.515 kb on + strand, within GFF1739 at 40.515 kb on + strand, within GFF1739 at 40.630 kb on - strand at 40.725 kb on + strand at 40.807 kb on - strand at 40.824 kb on + strand at 40.898 kb on - strand at 40.932 kb on - strand, within GFF1740 at 41.151 kb on + strand, within GFF1740 at 41.438 kb on + strand, within GFF1740 at 41.438 kb on + strand, within GFF1740 at 41.438 kb on + strand, within GFF1740 at 41.439 kb on - strand, within GFF1740 at 41.443 kb on + strand, within GFF1740 at 41.443 kb on + strand, within GFF1740 at 41.444 kb on - strand, within GFF1740 at 41.452 kb on - strand, within GFF1740 at 41.661 kb on + strand, within GFF1740 at 41.661 kb on + strand, within GFF1740 at 41.662 kb on - strand, within GFF1740 at 41.662 kb on - strand, within GFF1740 at 41.713 kb on + strand, within GFF1740 at 41.715 kb on + strand, within GFF1740 at 41.796 kb on + strand, within GFF1740 at 41.797 kb on - strand, within GFF1740 at 41.833 kb on + strand, within GFF1740 at 41.895 kb on - strand, within GFF1740 at 41.999 kb on - strand at 42.068 kb on + strand at 42.143 kb on - strand at 42.464 kb on - strand, within GFF1741 at 42.468 kb on - strand, within GFF1741 at 42.469 kb on + strand, within GFF1741 at 42.470 kb on - strand, within GFF1741 at 42.528 kb on + strand, within GFF1741 at 42.582 kb on - strand, within GFF1741 at 42.693 kb on - strand at 42.716 kb on + strand at 42.717 kb on - strand at 42.717 kb on - strand at 42.717 kb on - strand at 42.736 kb on - strand at 42.754 kb on - strand at 42.908 kb on + strand, within GFF1742 at 43.035 kb on - strand, within GFF1742 at 43.103 kb on + strand, within GFF1742
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 39,806 + -0.7 39,880 + +0.2 39,886 + -0.4 39,935 + -0.4 39,942 + +0.1 39,993 + -0.2 40,029 + GFF1739 0.14 -0.3 40,239 + GFF1739 0.46 -0.4 40,300 - GFF1739 0.55 +0.6 40,300 - GFF1739 0.55 +0.2 40,300 - GFF1739 0.55 +0.4 40,454 - GFF1739 0.78 -1.0 40,454 - GFF1739 0.78 +0.2 40,474 + GFF1739 0.81 -0.7 40,474 + GFF1739 0.81 -1.6 40,475 - GFF1739 0.81 -0.0 40,494 + GFF1739 0.84 -0.4 40,495 - GFF1739 0.84 -0.8 40,515 + GFF1739 0.87 +0.2 40,515 + GFF1739 0.87 -0.2 40,630 - +0.4 40,725 + +0.1 40,807 - -0.5 40,824 + +0.2 40,898 - -0.2 40,932 - GFF1740 0.12 -0.1 41,151 + GFF1740 0.29 -0.6 41,438 + GFF1740 0.50 +0.1 41,438 + GFF1740 0.50 +0.1 41,438 + GFF1740 0.50 -0.6 41,439 - GFF1740 0.50 -0.1 41,443 + GFF1740 0.50 -0.6 41,443 + GFF1740 0.50 -0.0 41,444 - GFF1740 0.51 -0.6 41,452 - GFF1740 0.51 +1.0 41,661 + GFF1740 0.67 +0.1 41,661 + GFF1740 0.67 -0.1 41,662 - GFF1740 0.67 +0.1 41,662 - GFF1740 0.67 +0.1 41,713 + GFF1740 0.71 -0.2 41,715 + GFF1740 0.71 +0.1 41,796 + GFF1740 0.77 -0.0 41,797 - GFF1740 0.77 -0.3 41,833 + GFF1740 0.80 -0.5 41,895 - GFF1740 0.84 -0.1 41,999 - -0.9 42,068 + -1.1 42,143 - +0.7 42,464 - GFF1741 0.54 -0.4 42,468 - GFF1741 0.54 -1.8 42,469 + GFF1741 0.55 +3.2 42,470 - GFF1741 0.55 +0.3 42,528 + GFF1741 0.65 +0.2 42,582 - GFF1741 0.74 -0.7 42,693 - -0.1 42,716 + +0.1 42,717 - -0.1 42,717 - +0.3 42,717 - +0.2 42,736 - +1.0 42,754 - -1.0 42,908 + GFF1742 0.23 +0.3 43,035 - GFF1742 0.40 +0.2 43,103 + GFF1742 0.49 +0.1
Or see this region's nucleotide sequence