Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1669 and GFF1670 are separated by 10 nucleotides GFF1670 and GFF1671 overlap by 4 nucleotides
GFF1669 - Signal transduction histidine kinase CheA (EC 2.7.3.-), at 53,599 to 55,608
GFF1669
GFF1670 - Flagellar motor rotation protein MotB, at 55,619 to 56,548
GFF1670
GFF1671 - Flagellar motor rotation protein MotA, at 56,545 to 57,432
GFF1671
Position (kb) 55
56
57 Strain fitness (log2 ratio) -2
-1
0
1 at 54.634 kb on + strand, within GFF1669 at 54.722 kb on - strand, within GFF1669 at 55.111 kb on + strand, within GFF1669 at 55.112 kb on - strand, within GFF1669 at 55.184 kb on - strand, within GFF1669 at 55.263 kb on - strand, within GFF1669 at 55.327 kb on - strand, within GFF1669 at 55.599 kb on + strand at 55.600 kb on - strand at 55.683 kb on - strand at 55.683 kb on - strand at 55.692 kb on - strand at 55.751 kb on + strand, within GFF1670 at 55.768 kb on + strand, within GFF1670 at 55.768 kb on + strand, within GFF1670 at 55.839 kb on - strand, within GFF1670 at 55.922 kb on - strand, within GFF1670 at 55.988 kb on + strand, within GFF1670 at 55.989 kb on - strand, within GFF1670 at 55.989 kb on - strand, within GFF1670 at 56.032 kb on + strand, within GFF1670 at 56.278 kb on - strand, within GFF1670 at 56.614 kb on - strand at 56.809 kb on - strand, within GFF1671 at 56.813 kb on - strand, within GFF1671 at 56.813 kb on - strand, within GFF1671 at 56.813 kb on - strand, within GFF1671 at 57.016 kb on - strand, within GFF1671 at 57.210 kb on - strand, within GFF1671 at 57.210 kb on - strand, within GFF1671 at 57.213 kb on + strand, within GFF1671 at 57.214 kb on - strand, within GFF1671 at 57.347 kb on + strand at 57.426 kb on + strand at 57.427 kb on - strand at 57.457 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 54,634 + GFF1669 0.51 -0.8 54,722 - GFF1669 0.56 +0.4 55,111 + GFF1669 0.75 +1.4 55,112 - GFF1669 0.75 +0.0 55,184 - GFF1669 0.79 -1.9 55,263 - GFF1669 0.83 +0.4 55,327 - GFF1669 0.86 -0.5 55,599 + -0.7 55,600 - -0.1 55,683 - +0.4 55,683 - -0.4 55,692 - -0.7 55,751 + GFF1670 0.14 -1.3 55,768 + GFF1670 0.16 -1.1 55,768 + GFF1670 0.16 -0.6 55,839 - GFF1670 0.24 +0.8 55,922 - GFF1670 0.33 -0.1 55,988 + GFF1670 0.40 -1.3 55,989 - GFF1670 0.40 -0.0 55,989 - GFF1670 0.40 -0.5 56,032 + GFF1670 0.44 -0.8 56,278 - GFF1670 0.71 +0.1 56,614 - -0.0 56,809 - GFF1671 0.30 -0.7 56,813 - GFF1671 0.30 -0.2 56,813 - GFF1671 0.30 -0.9 56,813 - GFF1671 0.30 +1.0 57,016 - GFF1671 0.53 -0.7 57,210 - GFF1671 0.75 -0.6 57,210 - GFF1671 0.75 -0.1 57,213 + GFF1671 0.75 +0.4 57,214 - GFF1671 0.75 +0.0 57,347 + +0.2 57,426 + -0.8 57,427 - +0.9 57,457 - +0.7
Or see this region's nucleotide sequence