Experiment: LB_plus_SM_buffer with 6C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1660 and GFF1661 overlap by 8 nucleotides GFF1661 and GFF1662 are separated by 193 nucleotides GFF1662 and GFF1663 are separated by 10 nucleotides
GFF1660 - Flagellar biosynthesis protein FlhA, at 44,633 to 46,711
GFF1660
GFF1661 - Flagellar biosynthesis protein FlhB, at 46,704 to 47,855
GFF1661
GFF1662 - Chemotaxis response - phosphatase CheZ, at 48,049 to 48,693
GFF1662
GFF1663 - Chemotaxis regulator - transmits chemoreceptor signals to flagelllar motor components CheY, at 48,704 to 49,093
GFF1663
Position (kb) 46
47
48 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 46.105 kb on + strand, within GFF1660 at 46.234 kb on + strand, within GFF1660 at 46.234 kb on + strand, within GFF1660 at 46.574 kb on + strand at 46.715 kb on + strand at 46.715 kb on + strand at 46.847 kb on - strand, within GFF1661 at 46.848 kb on + strand, within GFF1661 at 46.848 kb on + strand, within GFF1661 at 46.888 kb on + strand, within GFF1661 at 46.891 kb on - strand, within GFF1661 at 46.969 kb on - strand, within GFF1661 at 47.135 kb on + strand, within GFF1661 at 47.454 kb on - strand, within GFF1661 at 47.540 kb on + strand, within GFF1661 at 47.541 kb on - strand, within GFF1661 at 47.880 kb on - strand at 47.924 kb on - strand at 47.957 kb on + strand at 48.203 kb on - strand, within GFF1662 at 48.312 kb on + strand, within GFF1662 at 48.627 kb on + strand, within GFF1662 at 48.778 kb on - strand, within GFF1663
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 6C2_phage 15 MOI remove 46,105 + GFF1660 0.71 -3.0 46,234 + GFF1660 0.77 -3.5 46,234 + GFF1660 0.77 -0.0 46,574 + -0.8 46,715 + -2.5 46,715 + -0.4 46,847 - GFF1661 0.12 -0.9 46,848 + GFF1661 0.12 -0.8 46,848 + GFF1661 0.12 -0.5 46,888 + GFF1661 0.16 -0.7 46,891 - GFF1661 0.16 -0.7 46,969 - GFF1661 0.23 -0.1 47,135 + GFF1661 0.37 -0.8 47,454 - GFF1661 0.65 -0.4 47,540 + GFF1661 0.73 +0.0 47,541 - GFF1661 0.73 -0.0 47,880 - -0.1 47,924 - -0.7 47,957 + +0.3 48,203 - GFF1662 0.24 +1.7 48,312 + GFF1662 0.41 -1.3 48,627 + GFF1662 0.90 -0.2 48,778 - GFF1663 0.19 +0.3
Or see this region's nucleotide sequence