Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3217 and Atu3218 are separated by 125 nucleotides Atu3218 and Atu8029 are separated by 169 nucleotides Atu8029 and Atu3219 are separated by 81 nucleotides Atu3219 and Atu3221 are separated by 243 nucleotides Atu3221 and Atu3222 are separated by 92 nucleotides
Atu3217: Atu3217 - aspartate aminotransferase, at 231,610 to 232,728
Atu3217
Atu3218: Atu3218 - hypothetical protein, at 232,854 to 233,129
Atu3218
Atu8029: Atu8029 - hypothetical protein, at 233,299 to 233,427
Atu8029
Atu3219: Atu3219 - dihydroxy-acid dehydratase, at 233,509 to 235,320
Atu3219
Atu3221: Atu3221 - hypothetical protein, at 235,564 to 235,752
Atu3221
Atu3222: Atu3222 - ribose ABC transporter substrate-binding protein, at 235,845 to 236,804
Atu3222
Position (kb) 233
234
235
236 Strain fitness (log2 ratio) -1
0
1
2 at 232.546 kb on - strand, within Atu3217 at 232.580 kb on - strand, within Atu3217 at 232.768 kb on + strand at 232.918 kb on + strand, within Atu3218 at 232.918 kb on + strand, within Atu3218 at 232.926 kb on - strand, within Atu3218 at 233.071 kb on - strand, within Atu3218 at 233.190 kb on - strand at 233.203 kb on + strand at 233.213 kb on + strand at 233.224 kb on + strand at 233.232 kb on - strand at 233.232 kb on - strand at 233.232 kb on - strand at 233.234 kb on + strand at 233.378 kb on + strand, within Atu8029 at 233.476 kb on - strand at 233.530 kb on + strand at 233.531 kb on + strand at 233.531 kb on + strand at 233.531 kb on + strand at 233.531 kb on + strand at 233.531 kb on + strand at 233.539 kb on - strand at 233.539 kb on - strand at 233.593 kb on - strand at 233.593 kb on - strand at 233.646 kb on + strand at 233.647 kb on + strand at 233.655 kb on - strand at 233.664 kb on - strand at 233.692 kb on + strand, within Atu3219 at 233.692 kb on + strand, within Atu3219 at 233.718 kb on - strand, within Atu3219 at 233.741 kb on - strand, within Atu3219 at 233.770 kb on - strand, within Atu3219 at 233.819 kb on - strand, within Atu3219 at 234.023 kb on + strand, within Atu3219 at 234.101 kb on - strand, within Atu3219 at 234.191 kb on - strand, within Atu3219 at 234.285 kb on - strand, within Atu3219 at 234.290 kb on - strand, within Atu3219 at 234.329 kb on + strand, within Atu3219 at 234.339 kb on - strand, within Atu3219 at 234.449 kb on - strand, within Atu3219 at 234.463 kb on - strand, within Atu3219 at 234.480 kb on - strand, within Atu3219 at 234.506 kb on + strand, within Atu3219 at 234.549 kb on + strand, within Atu3219 at 234.554 kb on + strand, within Atu3219 at 234.643 kb on - strand, within Atu3219 at 234.720 kb on + strand, within Atu3219 at 234.750 kb on - strand, within Atu3219 at 234.846 kb on - strand, within Atu3219 at 234.851 kb on - strand, within Atu3219 at 234.851 kb on - strand, within Atu3219 at 234.916 kb on - strand, within Atu3219 at 234.944 kb on - strand, within Atu3219 at 234.967 kb on - strand, within Atu3219 at 234.977 kb on - strand, within Atu3219 at 234.987 kb on + strand, within Atu3219 at 234.991 kb on - strand, within Atu3219 at 235.093 kb on - strand, within Atu3219 at 235.124 kb on - strand, within Atu3219 at 235.145 kb on - strand at 235.150 kb on - strand at 235.160 kb on - strand at 235.344 kb on + strand at 235.462 kb on - strand at 235.823 kb on + strand at 235.829 kb on - strand at 235.889 kb on - strand at 235.960 kb on + strand, within Atu3222 at 235.965 kb on + strand, within Atu3222 at 236.013 kb on - strand, within Atu3222 at 236.068 kb on + strand, within Atu3222 at 236.097 kb on - strand, within Atu3222 at 236.102 kb on - strand, within Atu3222 at 236.267 kb on + strand, within Atu3222
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 232,546 - Atu3217 0.84 -0.1 232,580 - Atu3217 0.87 +0.3 232,768 + -0.4 232,918 + Atu3218 0.23 -0.1 232,918 + Atu3218 0.23 +1.0 232,926 - Atu3218 0.26 -0.2 233,071 - Atu3218 0.79 -0.2 233,190 - +0.4 233,203 + +0.8 233,213 + +0.2 233,224 + +0.7 233,232 - +0.5 233,232 - +0.3 233,232 - -0.1 233,234 + -0.5 233,378 + Atu8029 0.61 -0.1 233,476 - +0.5 233,530 + +0.9 233,531 + -0.1 233,531 + +0.2 233,531 + +0.7 233,531 + +0.6 233,531 + +0.0 233,539 - +0.4 233,539 - +0.4 233,593 - -0.1 233,593 - -1.3 233,646 + -0.0 233,647 + +0.6 233,655 - +0.3 233,664 - +0.8 233,692 + Atu3219 0.10 +0.1 233,692 + Atu3219 0.10 +0.1 233,718 - Atu3219 0.12 +0.1 233,741 - Atu3219 0.13 +0.3 233,770 - Atu3219 0.14 -0.8 233,819 - Atu3219 0.17 +0.9 234,023 + Atu3219 0.28 +0.4 234,101 - Atu3219 0.33 +0.1 234,191 - Atu3219 0.38 -0.5 234,285 - Atu3219 0.43 +0.2 234,290 - Atu3219 0.43 +0.3 234,329 + Atu3219 0.45 +0.5 234,339 - Atu3219 0.46 +0.2 234,449 - Atu3219 0.52 +0.2 234,463 - Atu3219 0.53 +0.0 234,480 - Atu3219 0.54 +1.2 234,506 + Atu3219 0.55 +0.1 234,549 + Atu3219 0.57 +0.9 234,554 + Atu3219 0.58 -0.8 234,643 - Atu3219 0.63 +0.1 234,720 + Atu3219 0.67 +2.2 234,750 - Atu3219 0.68 -0.6 234,846 - Atu3219 0.74 -0.4 234,851 - Atu3219 0.74 -0.6 234,851 - Atu3219 0.74 +0.4 234,916 - Atu3219 0.78 +0.1 234,944 - Atu3219 0.79 +0.2 234,967 - Atu3219 0.80 -0.9 234,977 - Atu3219 0.81 +0.5 234,987 + Atu3219 0.82 +1.1 234,991 - Atu3219 0.82 -0.0 235,093 - Atu3219 0.87 +0.3 235,124 - Atu3219 0.89 -0.4 235,145 - -0.8 235,150 - +0.1 235,160 - -0.2 235,344 + -0.2 235,462 - +1.1 235,823 + +0.4 235,829 - +0.5 235,889 - +0.2 235,960 + Atu3222 0.12 +0.9 235,965 + Atu3222 0.12 -0.5 236,013 - Atu3222 0.17 +0.2 236,068 + Atu3222 0.23 +0.0 236,097 - Atu3222 0.26 +0.0 236,102 - Atu3222 0.27 -0.1 236,267 + Atu3222 0.44 +0.1
Or see this region's nucleotide sequence