Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4063 and Atu4064 are separated by 135 nucleotides Atu4064 and Atu4065 are separated by 1 nucleotides Atu4065 and Atu4066 are separated by 96 nucleotides Atu4066 and Atu4067 are separated by 14 nucleotides Atu4067 and Atu4068 are separated by 11 nucleotides Atu4068 and Atu4069 overlap by 4 nucleotides
Atu4063: Atu4063 - O-linked GlcNAc transferase, at 1,180,552 to 1,181,448
Atu4063
Atu4064: Atu4064 - 30S ribosomal protein S21, at 1,181,584 to 1,181,811
Atu4064
Atu4065: Atu4065 - hypothetical protein, at 1,181,813 to 1,182,031
Atu4065
Atu4066: Atu4066 - sarcosine oxidase beta subunit, at 1,182,128 to 1,183,381
Atu4066
Atu4067: Atu4067 - hypothetical protein, at 1,183,396 to 1,183,791
Atu4067
Atu4068: Atu4068 - sarcosine oxidase delta subunit, at 1,183,803 to 1,184,117
Atu4068
Atu4069: Atu4069 - sarcosine oxidase alpha subunit, at 1,184,114 to 1,187,074
Atu4069
Position (kb)
1182
1183
1184 Strain fitness (log2 ratio)
-2
-1
0
1 at 1181.138 kb on + strand, within Atu4063 at 1181.146 kb on - strand, within Atu4063 at 1181.179 kb on + strand, within Atu4063 at 1181.187 kb on - strand, within Atu4063 at 1181.207 kb on - strand, within Atu4063 at 1181.243 kb on - strand, within Atu4063 at 1181.277 kb on - strand, within Atu4063 at 1181.345 kb on + strand, within Atu4063 at 1181.351 kb on - strand, within Atu4063 at 1181.353 kb on - strand, within Atu4063 at 1181.361 kb on - strand at 1181.380 kb on - strand at 1181.623 kb on - strand, within Atu4064 at 1182.064 kb on + strand at 1182.064 kb on + strand at 1182.064 kb on + strand at 1182.076 kb on + strand at 1182.076 kb on + strand at 1182.086 kb on + strand at 1182.199 kb on + strand at 1182.223 kb on + strand at 1182.269 kb on + strand, within Atu4066 at 1182.269 kb on + strand, within Atu4066 at 1182.269 kb on + strand, within Atu4066 at 1182.337 kb on + strand, within Atu4066 at 1182.359 kb on + strand, within Atu4066 at 1182.431 kb on + strand, within Atu4066 at 1182.583 kb on + strand, within Atu4066 at 1182.591 kb on - strand, within Atu4066 at 1182.634 kb on + strand, within Atu4066 at 1182.689 kb on + strand, within Atu4066 at 1182.697 kb on - strand, within Atu4066 at 1182.710 kb on + strand, within Atu4066 at 1182.710 kb on + strand, within Atu4066 at 1182.718 kb on - strand, within Atu4066 at 1182.734 kb on + strand, within Atu4066 at 1182.742 kb on - strand, within Atu4066 at 1182.878 kb on - strand, within Atu4066 at 1182.923 kb on - strand, within Atu4066 at 1182.924 kb on - strand, within Atu4066 at 1182.948 kb on + strand, within Atu4066 at 1182.961 kb on - strand, within Atu4066 at 1182.968 kb on + strand, within Atu4066 at 1182.978 kb on + strand, within Atu4066 at 1183.030 kb on + strand, within Atu4066 at 1183.063 kb on - strand, within Atu4066 at 1183.124 kb on - strand, within Atu4066 at 1183.148 kb on + strand, within Atu4066 at 1183.153 kb on + strand, within Atu4066 at 1183.153 kb on + strand, within Atu4066 at 1183.242 kb on - strand, within Atu4066 at 1183.289 kb on + strand at 1183.327 kb on + strand at 1183.327 kb on + strand at 1183.335 kb on - strand at 1183.386 kb on + strand at 1183.418 kb on - strand at 1183.450 kb on + strand, within Atu4067 at 1183.521 kb on - strand, within Atu4067 at 1183.605 kb on + strand, within Atu4067 at 1183.613 kb on - strand, within Atu4067 at 1183.613 kb on - strand, within Atu4067 at 1183.614 kb on - strand, within Atu4067 at 1183.665 kb on + strand, within Atu4067 at 1183.675 kb on + strand, within Atu4067 at 1183.702 kb on + strand, within Atu4067 at 1183.838 kb on - strand, within Atu4068 at 1183.866 kb on + strand, within Atu4068 at 1184.046 kb on + strand, within Atu4068 at 1184.158 kb on + strand at 1184.158 kb on + strand at 1184.244 kb on - strand at 1184.244 kb on - strand at 1184.366 kb on - strand at 1184.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 1,181,138 + Atu4063 0.65 +0.6 1,181,146 - Atu4063 0.66 -0.9 1,181,179 + Atu4063 0.70 +0.2 1,181,187 - Atu4063 0.71 -0.1 1,181,207 - Atu4063 0.73 +0.1 1,181,243 - Atu4063 0.77 -0.2 1,181,277 - Atu4063 0.81 +0.4 1,181,345 + Atu4063 0.88 -0.4 1,181,351 - Atu4063 0.89 +0.0 1,181,353 - Atu4063 0.89 +0.1 1,181,361 - +0.4 1,181,380 - +0.2 1,181,623 - Atu4064 0.17 -2.0 1,182,064 + +1.7 1,182,064 + +0.0 1,182,064 + -0.3 1,182,076 + -0.7 1,182,076 + +0.2 1,182,086 + +0.3 1,182,199 + +0.0 1,182,223 + +0.0 1,182,269 + Atu4066 0.11 -0.2 1,182,269 + Atu4066 0.11 +0.3 1,182,269 + Atu4066 0.11 -0.1 1,182,337 + Atu4066 0.17 +0.3 1,182,359 + Atu4066 0.18 +0.4 1,182,431 + Atu4066 0.24 -0.0 1,182,583 + Atu4066 0.36 +0.3 1,182,591 - Atu4066 0.37 -0.5 1,182,634 + Atu4066 0.40 +0.2 1,182,689 + Atu4066 0.45 +0.1 1,182,697 - Atu4066 0.45 +0.4 1,182,710 + Atu4066 0.46 +0.4 1,182,710 + Atu4066 0.46 -0.2 1,182,718 - Atu4066 0.47 +0.4 1,182,734 + Atu4066 0.48 +0.9 1,182,742 - Atu4066 0.49 +0.7 1,182,878 - Atu4066 0.60 +1.1 1,182,923 - Atu4066 0.63 +0.5 1,182,924 - Atu4066 0.63 +0.9 1,182,948 + Atu4066 0.65 +0.5 1,182,961 - Atu4066 0.66 +0.5 1,182,968 + Atu4066 0.67 +0.8 1,182,978 + Atu4066 0.68 +0.6 1,183,030 + Atu4066 0.72 +0.7 1,183,063 - Atu4066 0.75 -0.1 1,183,124 - Atu4066 0.79 +0.1 1,183,148 + Atu4066 0.81 +0.6 1,183,153 + Atu4066 0.82 +0.2 1,183,153 + Atu4066 0.82 +0.3 1,183,242 - Atu4066 0.89 +0.1 1,183,289 + +0.5 1,183,327 + -0.0 1,183,327 + -0.2 1,183,335 - +0.8 1,183,386 + +0.3 1,183,418 - +0.1 1,183,450 + Atu4067 0.14 +0.3 1,183,521 - Atu4067 0.32 +1.2 1,183,605 + Atu4067 0.53 -0.4 1,183,613 - Atu4067 0.55 -0.8 1,183,613 - Atu4067 0.55 +0.6 1,183,614 - Atu4067 0.55 -0.1 1,183,665 + Atu4067 0.68 +0.3 1,183,675 + Atu4067 0.70 -0.2 1,183,702 + Atu4067 0.77 -0.2 1,183,838 - Atu4068 0.11 +0.6 1,183,866 + Atu4068 0.20 +0.8 1,184,046 + Atu4068 0.77 -0.9 1,184,158 + -0.1 1,184,158 + +0.3 1,184,244 - +0.6 1,184,244 - -0.2 1,184,366 - +0.4 1,184,378 + +0.2
Or see this region's nucleotide sequence