Experiment: R2A outgrowth control
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABID97_RS13190 and ABID97_RS13195 are separated by 135 nucleotides ABID97_RS13195 and ABID97_RS13200 are separated by 19 nucleotides ABID97_RS13200 and ABID97_RS13205 are separated by 12 nucleotides
ABID97_RS13190: ABID97_RS13190 - ABC transporter substrate-binding protein, at 2,747,301 to 2,748,479
_RS13190
ABID97_RS13195: ABID97_RS13195 - maleylacetate reductase, at 2,748,615 to 2,749,691
_RS13195
ABID97_RS13200: ABID97_RS13200 - intradiol ring-cleavage dioxygenase, at 2,749,711 to 2,750,559
_RS13200
ABID97_RS13205: ABID97_RS13205 - tripartite tricarboxylate transporter substrate binding protein, at 2,750,572 to 2,751,534
_RS13205
Position (kb)
2748
2749
2750 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2747.701 kb on + strand, within ABID97_RS13190 at 2747.764 kb on + strand, within ABID97_RS13190 at 2747.813 kb on - strand, within ABID97_RS13190 at 2748.046 kb on + strand, within ABID97_RS13190 at 2748.046 kb on + strand, within ABID97_RS13190 at 2748.064 kb on + strand, within ABID97_RS13190 at 2748.313 kb on + strand, within ABID97_RS13190 at 2748.314 kb on - strand, within ABID97_RS13190 at 2749.153 kb on + strand, within ABID97_RS13195 at 2749.154 kb on - strand, within ABID97_RS13195 at 2749.336 kb on + strand, within ABID97_RS13195 at 2749.769 kb on + strand at 2749.769 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A outgrowth control remove 2,747,701 + ABID97_RS13190 0.34 -1.3 2,747,764 + ABID97_RS13190 0.39 +0.1 2,747,813 - ABID97_RS13190 0.43 +0.5 2,748,046 + ABID97_RS13190 0.63 -0.3 2,748,046 + ABID97_RS13190 0.63 -0.3 2,748,064 + ABID97_RS13190 0.65 -0.1 2,748,313 + ABID97_RS13190 0.86 -0.5 2,748,314 - ABID97_RS13190 0.86 +0.2 2,749,153 + ABID97_RS13195 0.50 +0.2 2,749,154 - ABID97_RS13195 0.50 -1.9 2,749,336 + ABID97_RS13195 0.67 -0.2 2,749,769 + -3.0 2,749,769 + -0.2
Or see this region's nucleotide sequence