Experiment: L-Threonine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PfGW456L13_141 and PfGW456L13_142 are separated by 28 nucleotides PfGW456L13_142 and PfGW456L13_143 are separated by 305 nucleotides PfGW456L13_143 and PfGW456L13_144 overlap by 4 nucleotides
PfGW456L13_141 - SSU ribosomal protein S18p @ SSU ribosomal protein S18p, zinc-independent, at 162,311 to 162,541
_141
PfGW456L13_142 - SSU ribosomal protein S6p, at 162,570 to 162,992
_142
PfGW456L13_143 - 23S rRNA (guanosine-2'-O-) -methyltransferase rlmB (EC 2.1.1.-), at 163,298 to 164,065
_143
PfGW456L13_144 - 3'-to-5' exoribonuclease RNase R, at 164,062 to 166,689
_144
Position (kb) 163
164
165 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 163.142 kb on + strand at 163.143 kb on - strand at 163.154 kb on - strand at 163.182 kb on + strand at 163.183 kb on - strand at 163.237 kb on + strand at 163.237 kb on + strand at 163.237 kb on + strand at 163.238 kb on - strand at 163.238 kb on - strand at 163.250 kb on + strand at 163.250 kb on + strand at 163.426 kb on - strand, within PfGW456L13_143 at 163.512 kb on + strand, within PfGW456L13_143 at 163.513 kb on - strand, within PfGW456L13_143 at 163.513 kb on - strand, within PfGW456L13_143 at 163.865 kb on + strand, within PfGW456L13_143 at 163.865 kb on + strand, within PfGW456L13_143 at 163.865 kb on + strand, within PfGW456L13_143 at 163.865 kb on + strand, within PfGW456L13_143 at 163.866 kb on - strand, within PfGW456L13_143 at 164.027 kb on + strand at 164.027 kb on + strand at 164.027 kb on + strand at 164.028 kb on - strand at 164.028 kb on - strand at 164.028 kb on - strand at 164.036 kb on + strand at 164.036 kb on + strand at 164.036 kb on + strand at 164.036 kb on + strand at 164.036 kb on + strand at 164.036 kb on + strand at 164.036 kb on + strand at 164.037 kb on - strand at 164.037 kb on - strand at 164.037 kb on - strand at 164.037 kb on - strand at 164.037 kb on - strand at 164.037 kb on - strand at 164.037 kb on - strand at 164.037 kb on - strand at 164.043 kb on + strand at 164.043 kb on + strand at 164.043 kb on + strand at 164.043 kb on + strand at 164.044 kb on - strand at 164.044 kb on - strand at 164.044 kb on - strand at 164.077 kb on - strand at 164.134 kb on - strand at 164.317 kb on + strand at 164.317 kb on + strand at 164.317 kb on + strand at 164.318 kb on - strand at 164.577 kb on - strand, within PfGW456L13_144 at 164.577 kb on - strand, within PfGW456L13_144 at 164.579 kb on + strand, within PfGW456L13_144 at 164.621 kb on + strand, within PfGW456L13_144 at 164.621 kb on + strand, within PfGW456L13_144 at 164.621 kb on + strand, within PfGW456L13_144 at 164.621 kb on + strand, within PfGW456L13_144 at 164.625 kb on + strand, within PfGW456L13_144 at 164.626 kb on - strand, within PfGW456L13_144 at 164.731 kb on + strand, within PfGW456L13_144 at 164.731 kb on + strand, within PfGW456L13_144 at 164.731 kb on + strand, within PfGW456L13_144 at 164.731 kb on + strand, within PfGW456L13_144 at 164.732 kb on - strand, within PfGW456L13_144 at 164.732 kb on - strand, within PfGW456L13_144 at 164.732 kb on - strand, within PfGW456L13_144 at 164.954 kb on + strand, within PfGW456L13_144
Per-strain Table
Position Strand Gene LocusTag Fraction L-Threonine (N) remove 163,142 + -0.8 163,143 - -0.0 163,154 - -1.3 163,182 + +0.1 163,183 - -1.5 163,237 + +0.1 163,237 + +0.5 163,237 + +1.0 163,238 - -1.7 163,238 - -0.0 163,250 + -0.6 163,250 + +0.7 163,426 - PfGW456L13_143 0.17 +0.7 163,512 + PfGW456L13_143 0.28 +0.4 163,513 - PfGW456L13_143 0.28 -0.2 163,513 - PfGW456L13_143 0.28 -2.1 163,865 + PfGW456L13_143 0.74 -0.8 163,865 + PfGW456L13_143 0.74 +1.1 163,865 + PfGW456L13_143 0.74 +0.7 163,865 + PfGW456L13_143 0.74 +1.1 163,866 - PfGW456L13_143 0.74 +1.2 164,027 + -0.4 164,027 + +0.5 164,027 + +0.3 164,028 - +0.3 164,028 - +0.4 164,028 - +1.0 164,036 + +1.2 164,036 + -0.4 164,036 + -0.3 164,036 + -0.4 164,036 + +0.7 164,036 + -0.0 164,036 + +0.7 164,037 - +0.5 164,037 - +2.3 164,037 - +0.2 164,037 - +0.6 164,037 - -0.1 164,037 - +1.0 164,037 - -0.3 164,037 - -0.9 164,043 + +1.6 164,043 + +0.4 164,043 + -0.5 164,043 + +0.4 164,044 - +0.1 164,044 - +0.5 164,044 - -0.5 164,077 - -0.4 164,134 - +2.0 164,317 + +0.6 164,317 + +0.3 164,317 + -0.9 164,318 - -0.1 164,577 - PfGW456L13_144 0.20 +1.1 164,577 - PfGW456L13_144 0.20 +1.3 164,579 + PfGW456L13_144 0.20 +2.6 164,621 + PfGW456L13_144 0.21 +1.6 164,621 + PfGW456L13_144 0.21 +3.2 164,621 + PfGW456L13_144 0.21 +0.1 164,621 + PfGW456L13_144 0.21 +2.5 164,625 + PfGW456L13_144 0.21 -0.5 164,626 - PfGW456L13_144 0.21 +0.9 164,731 + PfGW456L13_144 0.25 +0.0 164,731 + PfGW456L13_144 0.25 -0.1 164,731 + PfGW456L13_144 0.25 +1.9 164,731 + PfGW456L13_144 0.25 +0.7 164,732 - PfGW456L13_144 0.25 +0.5 164,732 - PfGW456L13_144 0.25 +0.5 164,732 - PfGW456L13_144 0.25 +1.3 164,954 + PfGW456L13_144 0.34 +2.1
Or see this region's nucleotide sequence