Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF982 and GFF983 are separated by 238 nucleotides GFF983 and GFF984 are separated by 5 nucleotides
GFF982 - Putative cytoplasmic protein USSDB7A, at 166,993 to 167,439
GFF982
GFF983 - Aspartate racemase (EC 5.1.1.13), at 167,678 to 168,412
GFF983
GFF984 - LysR family transcriptional regulator QseA, at 168,418 to 169,326
GFF984
Position (kb) 167
168
169 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 166.787 kb on + strand at 166.843 kb on - strand at 167.125 kb on + strand, within GFF982 at 167.125 kb on + strand, within GFF982 at 167.125 kb on + strand, within GFF982 at 167.126 kb on - strand, within GFF982 at 167.126 kb on - strand, within GFF982 at 167.126 kb on - strand, within GFF982 at 167.127 kb on + strand, within GFF982 at 167.128 kb on - strand, within GFF982 at 167.166 kb on + strand, within GFF982 at 167.179 kb on + strand, within GFF982 at 167.228 kb on + strand, within GFF982 at 167.228 kb on + strand, within GFF982 at 167.263 kb on - strand, within GFF982 at 167.265 kb on + strand, within GFF982 at 167.271 kb on + strand, within GFF982 at 167.355 kb on + strand, within GFF982 at 167.362 kb on - strand, within GFF982 at 167.479 kb on + strand at 167.528 kb on + strand at 167.529 kb on - strand at 167.824 kb on + strand, within GFF983 at 167.885 kb on - strand, within GFF983 at 167.912 kb on - strand, within GFF983 at 167.912 kb on - strand, within GFF983 at 167.912 kb on - strand, within GFF983 at 167.940 kb on - strand, within GFF983 at 167.940 kb on - strand, within GFF983 at 168.075 kb on - strand, within GFF983 at 168.075 kb on - strand, within GFF983 at 168.108 kb on + strand, within GFF983 at 168.155 kb on + strand, within GFF983 at 168.244 kb on + strand, within GFF983 at 168.245 kb on - strand, within GFF983 at 168.271 kb on + strand, within GFF983 at 168.500 kb on + strand at 168.500 kb on + strand at 168.500 kb on + strand at 168.501 kb on - strand at 168.578 kb on + strand, within GFF984 at 168.579 kb on - strand, within GFF984 at 168.657 kb on + strand, within GFF984 at 168.712 kb on - strand, within GFF984 at 168.729 kb on - strand, within GFF984 at 168.765 kb on - strand, within GFF984 at 168.898 kb on + strand, within GFF984 at 168.899 kb on - strand, within GFF984 at 168.899 kb on - strand, within GFF984 at 168.925 kb on + strand, within GFF984 at 168.945 kb on + strand, within GFF984 at 168.954 kb on + strand, within GFF984 at 169.279 kb on + strand at 169.328 kb on + strand at 169.363 kb on + strand at 169.363 kb on + strand at 169.364 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 166,787 + +0.9 166,843 - -0.7 167,125 + GFF982 0.30 +0.1 167,125 + GFF982 0.30 -0.3 167,125 + GFF982 0.30 +0.4 167,126 - GFF982 0.30 +0.6 167,126 - GFF982 0.30 +0.2 167,126 - GFF982 0.30 -0.6 167,127 + GFF982 0.30 -0.5 167,128 - GFF982 0.30 -0.2 167,166 + GFF982 0.39 +0.2 167,179 + GFF982 0.42 -0.8 167,228 + GFF982 0.53 -0.1 167,228 + GFF982 0.53 -0.2 167,263 - GFF982 0.60 -0.6 167,265 + GFF982 0.61 -0.2 167,271 + GFF982 0.62 +0.6 167,355 + GFF982 0.81 +0.2 167,362 - GFF982 0.83 +0.1 167,479 + +0.1 167,528 + +0.9 167,529 - +0.2 167,824 + GFF983 0.20 +1.7 167,885 - GFF983 0.28 +0.3 167,912 - GFF983 0.32 +0.4 167,912 - GFF983 0.32 +0.1 167,912 - GFF983 0.32 -1.8 167,940 - GFF983 0.36 -0.1 167,940 - GFF983 0.36 +0.4 168,075 - GFF983 0.54 +2.3 168,075 - GFF983 0.54 +0.4 168,108 + GFF983 0.59 -1.8 168,155 + GFF983 0.65 -0.5 168,244 + GFF983 0.77 -0.5 168,245 - GFF983 0.77 -0.3 168,271 + GFF983 0.81 -0.5 168,500 + -1.3 168,500 + -0.2 168,500 + -1.3 168,501 - -0.2 168,578 + GFF984 0.18 +0.5 168,579 - GFF984 0.18 -0.3 168,657 + GFF984 0.26 -0.5 168,712 - GFF984 0.32 -0.4 168,729 - GFF984 0.34 -3.0 168,765 - GFF984 0.38 +0.4 168,898 + GFF984 0.53 -0.4 168,899 - GFF984 0.53 -2.3 168,899 - GFF984 0.53 -0.4 168,925 + GFF984 0.56 -1.6 168,945 + GFF984 0.58 +0.2 168,954 + GFF984 0.59 +0.7 169,279 + +0.4 169,328 + -0.4 169,363 + -0.2 169,363 + -0.7 169,364 - -1.3
Or see this region's nucleotide sequence