Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF915 and GFF916 are separated by 77 nucleotides GFF916 and GFF917 overlap by 11 nucleotides GFF917 and GFF918 are separated by 3 nucleotides GFF918 and GFF919 are separated by 120 nucleotides
GFF915 - Putative BglB-family transcriptional antiterminator, at 92,809 to 94,722
GFF915
GFF916 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 94,800 to 95,042
GFF916
GFF917 - RelE/StbE replicon stabilization toxin, at 95,032 to 95,316
GFF917
GFF918 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 95,320 to 95,784
GFF918
GFF919 - Ribonucleotide reductase of class III (anaerobic), large subunit (EC 1.17.4.2), at 95,905 to 98,043
GFF919
Position (kb) 95
96 Strain fitness (log2 ratio) -1
0
1
2 at 94.119 kb on - strand, within GFF915 at 94.229 kb on - strand, within GFF915 at 94.334 kb on + strand, within GFF915 at 94.441 kb on - strand, within GFF915 at 94.849 kb on - strand, within GFF916 at 94.898 kb on - strand, within GFF916 at 95.038 kb on + strand at 95.038 kb on + strand at 95.038 kb on + strand at 95.038 kb on + strand at 95.079 kb on + strand, within GFF917 at 95.080 kb on - strand, within GFF917 at 95.132 kb on + strand, within GFF917 at 95.132 kb on + strand, within GFF917 at 95.196 kb on + strand, within GFF917 at 95.315 kb on - strand at 95.485 kb on - strand, within GFF918 at 95.519 kb on - strand, within GFF918 at 95.519 kb on - strand, within GFF918 at 95.625 kb on + strand, within GFF918 at 95.626 kb on - strand, within GFF918 at 95.643 kb on + strand, within GFF918 at 95.684 kb on + strand, within GFF918 at 95.765 kb on + strand at 95.806 kb on + strand at 95.806 kb on + strand at 95.815 kb on + strand at 95.911 kb on + strand at 95.912 kb on - strand at 95.927 kb on - strand at 95.927 kb on - strand at 96.111 kb on - strand at 96.143 kb on + strand, within GFF919 at 96.148 kb on + strand, within GFF919 at 96.149 kb on - strand, within GFF919 at 96.164 kb on + strand, within GFF919 at 96.254 kb on + strand, within GFF919 at 96.254 kb on + strand, within GFF919 at 96.281 kb on + strand, within GFF919
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 94,119 - GFF915 0.68 -0.0 94,229 - GFF915 0.74 -1.7 94,334 + GFF915 0.80 +0.3 94,441 - GFF915 0.85 -1.3 94,849 - GFF916 0.20 +2.1 94,898 - GFF916 0.40 +0.5 95,038 + +0.2 95,038 + +0.5 95,038 + -0.5 95,038 + -0.4 95,079 + GFF917 0.16 -0.0 95,080 - GFF917 0.17 +0.2 95,132 + GFF917 0.35 -0.3 95,132 + GFF917 0.35 -1.4 95,196 + GFF917 0.58 -0.2 95,315 - -0.3 95,485 - GFF918 0.35 +0.0 95,519 - GFF918 0.43 +0.3 95,519 - GFF918 0.43 +0.1 95,625 + GFF918 0.66 +0.5 95,626 - GFF918 0.66 +0.2 95,643 + GFF918 0.69 -0.5 95,684 + GFF918 0.78 +0.1 95,765 + -0.0 95,806 + -0.3 95,806 + +0.3 95,815 + -0.4 95,911 + -0.5 95,912 - -0.3 95,927 - -0.0 95,927 - -0.3 96,111 - -1.4 96,143 + GFF919 0.11 -0.4 96,148 + GFF919 0.11 +0.3 96,149 - GFF919 0.11 -0.3 96,164 + GFF919 0.12 +0.3 96,254 + GFF919 0.16 +1.2 96,254 + GFF919 0.16 +0.4 96,281 + GFF919 0.18 -0.8
Or see this region's nucleotide sequence