Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF861 and GFF862 are separated by 111 nucleotides GFF862 and GFF863 are separated by 102 nucleotides GFF863 and GFF864 are separated by 80 nucleotides
GFF861 - D-serine/D-alanine/glycine transporter, at 38,197 to 39,606
GFF861
GFF862 - Nitric oxide-dependent regulator DnrN or NorA, at 39,718 to 40,380
GFF862
GFF863 - Putative membrane protein, at 40,483 to 41,448
GFF863
GFF864 - NADPH:quinone oxidoreductase 2, at 41,529 to 42,377
GFF864
Position (kb) 40
41
42 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 39.488 kb on + strand at 39.489 kb on - strand at 39.492 kb on - strand at 39.575 kb on + strand at 39.580 kb on - strand at 39.643 kb on - strand at 39.649 kb on + strand at 39.809 kb on + strand, within GFF862 at 39.809 kb on + strand, within GFF862 at 39.810 kb on - strand, within GFF862 at 39.851 kb on - strand, within GFF862 at 39.993 kb on + strand, within GFF862 at 39.994 kb on - strand, within GFF862 at 40.155 kb on + strand, within GFF862 at 40.156 kb on - strand, within GFF862 at 40.228 kb on + strand, within GFF862 at 40.229 kb on - strand, within GFF862 at 40.229 kb on - strand, within GFF862 at 40.229 kb on - strand, within GFF862 at 40.229 kb on - strand, within GFF862 at 40.303 kb on - strand, within GFF862 at 40.461 kb on + strand at 40.462 kb on - strand at 40.615 kb on - strand, within GFF863 at 40.615 kb on - strand, within GFF863 at 40.615 kb on - strand, within GFF863 at 40.615 kb on - strand, within GFF863 at 40.616 kb on + strand, within GFF863 at 41.110 kb on - strand, within GFF863 at 41.213 kb on + strand, within GFF863 at 41.216 kb on + strand, within GFF863 at 41.222 kb on - strand, within GFF863 at 41.354 kb on - strand at 41.430 kb on - strand at 41.431 kb on + strand at 41.431 kb on + strand at 41.595 kb on - strand at 41.651 kb on + strand, within GFF864 at 41.750 kb on + strand, within GFF864 at 41.973 kb on + strand, within GFF864 at 41.974 kb on - strand, within GFF864 at 41.991 kb on - strand, within GFF864 at 42.024 kb on - strand, within GFF864 at 42.063 kb on + strand, within GFF864 at 42.064 kb on - strand, within GFF864 at 42.075 kb on - strand, within GFF864 at 42.077 kb on - strand, within GFF864 at 42.077 kb on - strand, within GFF864 at 42.162 kb on + strand, within GFF864 at 42.164 kb on + strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.165 kb on - strand, within GFF864 at 42.177 kb on - strand, within GFF864 at 42.177 kb on - strand, within GFF864 at 42.319 kb on - strand at 42.368 kb on + strand at 42.411 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 39,488 + +0.5 39,489 - -0.0 39,492 - -0.3 39,575 + -0.1 39,580 - -1.6 39,643 - -0.3 39,649 + -0.0 39,809 + GFF862 0.14 +0.3 39,809 + GFF862 0.14 +1.3 39,810 - GFF862 0.14 +0.8 39,851 - GFF862 0.20 +0.0 39,993 + GFF862 0.41 +0.1 39,994 - GFF862 0.42 +0.2 40,155 + GFF862 0.66 +0.0 40,156 - GFF862 0.66 +0.5 40,228 + GFF862 0.77 -0.6 40,229 - GFF862 0.77 +0.4 40,229 - GFF862 0.77 -2.6 40,229 - GFF862 0.77 -0.1 40,229 - GFF862 0.77 +0.9 40,303 - GFF862 0.88 -0.3 40,461 + +0.5 40,462 - +1.1 40,615 - GFF863 0.14 -0.3 40,615 - GFF863 0.14 -1.1 40,615 - GFF863 0.14 -0.9 40,615 - GFF863 0.14 -0.3 40,616 + GFF863 0.14 -1.4 41,110 - GFF863 0.65 +0.1 41,213 + GFF863 0.76 -2.3 41,216 + GFF863 0.76 +0.1 41,222 - GFF863 0.77 -2.8 41,354 - -0.3 41,430 - +0.2 41,431 + -0.2 41,431 + -0.4 41,595 - +0.8 41,651 + GFF864 0.14 -0.1 41,750 + GFF864 0.26 -0.3 41,973 + GFF864 0.52 -0.3 41,974 - GFF864 0.52 +0.5 41,991 - GFF864 0.54 -0.3 42,024 - GFF864 0.58 -0.5 42,063 + GFF864 0.63 -0.0 42,064 - GFF864 0.63 +0.8 42,075 - GFF864 0.64 +0.1 42,077 - GFF864 0.65 -0.4 42,077 - GFF864 0.65 +0.5 42,162 + GFF864 0.75 +0.6 42,164 + GFF864 0.75 -0.4 42,165 - GFF864 0.75 +0.7 42,165 - GFF864 0.75 +0.2 42,165 - GFF864 0.75 +0.7 42,165 - GFF864 0.75 +0.7 42,165 - GFF864 0.75 -1.6 42,177 - GFF864 0.76 -0.1 42,177 - GFF864 0.76 +0.3 42,319 - +1.3 42,368 + -0.1 42,411 + -0.3
Or see this region's nucleotide sequence