Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF726 and GFF727 are separated by 10 nucleotides GFF727 and GFF728 are separated by 32 nucleotides
GFF726 - Isoaspartyl aminopeptidase (EC 3.4.19.5) @ Asp-X dipeptidase, at 84,213 to 85,154
GFF726
GFF727 - Dipeptide transport ATP-binding protein DppD in protein degradation cluster, at 85,165 to 87,036
GFF727
GFF728 - Dipeptide transport system, periplasmic component in protein degradation cluster, at 87,069 to 88,607
GFF728
Position (kb)
85
86
87
88 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 84.174 kb on + strand at 84.174 kb on + strand at 84.289 kb on + strand at 84.290 kb on - strand at 84.295 kb on - strand at 84.367 kb on + strand, within GFF726 at 84.367 kb on + strand, within GFF726 at 84.432 kb on + strand, within GFF726 at 84.433 kb on - strand, within GFF726 at 84.571 kb on + strand, within GFF726 at 84.572 kb on - strand, within GFF726 at 84.819 kb on - strand, within GFF726 at 84.819 kb on - strand, within GFF726 at 85.065 kb on - strand at 85.098 kb on + strand at 85.098 kb on + strand at 85.099 kb on - strand at 85.524 kb on - strand, within GFF727 at 85.527 kb on + strand, within GFF727 at 85.891 kb on + strand, within GFF727 at 85.923 kb on - strand, within GFF727 at 85.956 kb on + strand, within GFF727 at 86.100 kb on + strand, within GFF727 at 86.100 kb on + strand, within GFF727 at 86.100 kb on + strand, within GFF727 at 86.379 kb on + strand, within GFF727 at 86.721 kb on + strand, within GFF727 at 86.733 kb on + strand, within GFF727 at 86.734 kb on - strand, within GFF727 at 86.777 kb on - strand, within GFF727 at 86.777 kb on - strand, within GFF727 at 86.839 kb on + strand, within GFF727 at 86.910 kb on - strand at 86.910 kb on - strand at 86.921 kb on + strand at 87.032 kb on + strand at 87.032 kb on + strand at 87.033 kb on - strand at 87.035 kb on - strand at 87.090 kb on - strand at 87.198 kb on + strand at 87.213 kb on - strand at 87.303 kb on + strand, within GFF728 at 87.719 kb on + strand, within GFF728 at 87.867 kb on - strand, within GFF728 at 87.876 kb on + strand, within GFF728 at 87.876 kb on + strand, within GFF728 at 87.877 kb on - strand, within GFF728 at 87.877 kb on - strand, within GFF728 at 87.878 kb on + strand, within GFF728 at 87.885 kb on - strand, within GFF728 at 87.911 kb on + strand, within GFF728 at 87.940 kb on - strand, within GFF728
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 84,174 + +0.3 84,174 + +0.2 84,289 + +0.3 84,290 - -2.4 84,295 - +0.4 84,367 + GFF726 0.16 -1.2 84,367 + GFF726 0.16 +0.8 84,432 + GFF726 0.23 +0.2 84,433 - GFF726 0.23 +0.7 84,571 + GFF726 0.38 +0.5 84,572 - GFF726 0.38 -1.2 84,819 - GFF726 0.64 +1.0 84,819 - GFF726 0.64 -0.1 85,065 - +0.6 85,098 + +0.3 85,098 + +0.3 85,099 - +0.1 85,524 - GFF727 0.19 +0.5 85,527 + GFF727 0.19 -0.4 85,891 + GFF727 0.39 -0.3 85,923 - GFF727 0.40 +1.0 85,956 + GFF727 0.42 +0.6 86,100 + GFF727 0.50 +2.6 86,100 + GFF727 0.50 -0.1 86,100 + GFF727 0.50 +1.0 86,379 + GFF727 0.65 +1.5 86,721 + GFF727 0.83 -1.2 86,733 + GFF727 0.84 -0.2 86,734 - GFF727 0.84 +0.5 86,777 - GFF727 0.86 +0.4 86,777 - GFF727 0.86 -0.4 86,839 + GFF727 0.89 +0.4 86,910 - -0.5 86,910 - -0.7 86,921 + +0.1 87,032 + +0.3 87,032 + -0.4 87,033 - -0.8 87,035 - -0.8 87,090 - +0.5 87,198 + -0.4 87,213 - +0.6 87,303 + GFF728 0.15 -0.4 87,719 + GFF728 0.42 +0.9 87,867 - GFF728 0.52 +0.2 87,876 + GFF728 0.52 +0.3 87,876 + GFF728 0.52 -1.7 87,877 - GFF728 0.53 -1.6 87,877 - GFF728 0.53 -0.4 87,878 + GFF728 0.53 -0.7 87,885 - GFF728 0.53 -0.7 87,911 + GFF728 0.55 +0.0 87,940 - GFF728 0.57 -0.5
Or see this region's nucleotide sequence