Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF674

Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF672 and GFF673 overlap by 17 nucleotidesGFF673 and GFF674 overlap by 8 nucleotidesGFF674 and GFF675 are separated by 650 nucleotides GFF672 - 8-amino-7-oxononanoate synthase (EC 2.3.1.47), at 28,271 to 29,428 GFF672 GFF673 - Biotin synthesis protein BioC, at 29,412 to 30,167 GFF673 GFF674 - Dethiobiotin synthetase (EC 6.3.3.3), at 30,160 to 30,846 GFF674 GFF675 - Excinuclease ABC subunit B, at 31,497 to 33,518 GFF675 Position (kb) 30 31Strain fitness (log2 ratio) -2 -1 0 1 2at 29.426 kb on + strandat 29.426 kb on + strandat 29.577 kb on - strand, within GFF673at 29.577 kb on - strand, within GFF673at 29.577 kb on - strand, within GFF673at 29.790 kb on + strand, within GFF673at 29.791 kb on - strand, within GFF673at 30.080 kb on + strand, within GFF673at 30.197 kb on - strandat 30.249 kb on - strand, within GFF674at 30.249 kb on - strand, within GFF674at 30.264 kb on - strand, within GFF674at 30.264 kb on - strand, within GFF674at 30.268 kb on + strand, within GFF674at 30.268 kb on + strand, within GFF674at 30.271 kb on - strand, within GFF674at 30.384 kb on + strand, within GFF674at 30.399 kb on + strand, within GFF674at 30.399 kb on + strand, within GFF674at 30.525 kb on + strand, within GFF674at 30.615 kb on + strand, within GFF674at 30.615 kb on + strand, within GFF674at 30.616 kb on - strand, within GFF674at 30.618 kb on + strand, within GFF674at 30.692 kb on + strand, within GFF674at 30.693 kb on - strand, within GFF674at 30.693 kb on - strand, within GFF674at 30.815 kb on - strandat 30.826 kb on - strandat 31.109 kb on - strandat 31.358 kb on + strandat 31.392 kb on - strandat 31.392 kb on - strandat 31.502 kb on + strandat 31.556 kb on + strandat 31.770 kb on + strand, within GFF675at 31.770 kb on + strand, within GFF675at 31.773 kb on + strand, within GFF675at 31.797 kb on + strand, within GFF675at 31.798 kb on - strand, within GFF675

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI
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29,426 + +0.3
29,426 + +0.1
29,577 - GFF673 0.22 -0.4
29,577 - GFF673 0.22 -1.1
29,577 - GFF673 0.22 -0.1
29,790 + GFF673 0.50 -0.7
29,791 - GFF673 0.50 -0.5
30,080 + GFF673 0.88 -0.4
30,197 - +0.2
30,249 - GFF674 0.13 -0.1
30,249 - GFF674 0.13 -0.1
30,264 - GFF674 0.15 +0.4
30,264 - GFF674 0.15 -0.4
30,268 + GFF674 0.16 -0.4
30,268 + GFF674 0.16 -0.1
30,271 - GFF674 0.16 -0.4
30,384 + GFF674 0.33 +1.5
30,399 + GFF674 0.35 +0.1
30,399 + GFF674 0.35 -2.1
30,525 + GFF674 0.53 -0.4
30,615 + GFF674 0.66 -0.5
30,615 + GFF674 0.66 +0.0
30,616 - GFF674 0.66 +0.6
30,618 + GFF674 0.67 +1.3
30,692 + GFF674 0.77 +0.5
30,693 - GFF674 0.78 +1.5
30,693 - GFF674 0.78 +2.0
30,815 - +0.7
30,826 - -0.1
31,109 - -0.8
31,358 + +0.7
31,392 - -1.2
31,392 - +0.6
31,502 + -1.6
31,556 + +0.3
31,770 + GFF675 0.14 +2.2
31,770 + GFF675 0.14 +1.5
31,773 + GFF675 0.14 -0.6
31,797 + GFF675 0.15 +0.2
31,798 - GFF675 0.15 -0.2

Or see this region's nucleotide sequence