Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4860 and GFF4861 are separated by 1 nucleotides GFF4861 and GFF4862 are separated by 57 nucleotides GFF4862 and GFF4863 are separated by 58 nucleotides
GFF4860 - L-fuculokinase (EC 2.7.1.51), at 160,061 to 161,479
GFF4860
GFF4861 - L-fucose mutarotase, at 161,481 to 161,903
GFF4861
GFF4862 - L-fucose operon activator, at 161,961 to 162,671
GFF4862
GFF4863 - 'LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM' transl_table=11, at 162,730 to 163,830
GFF4863
Position (kb)
161
162
163 Strain fitness (log2 ratio)
-1
0
1 at 161.138 kb on - strand, within GFF4860 at 161.247 kb on + strand, within GFF4860 at 161.424 kb on - strand at 161.436 kb on - strand at 161.672 kb on + strand, within GFF4861 at 161.673 kb on - strand, within GFF4861 at 162.044 kb on + strand, within GFF4862 at 162.085 kb on + strand, within GFF4862 at 162.125 kb on + strand, within GFF4862 at 162.126 kb on - strand, within GFF4862 at 162.130 kb on - strand, within GFF4862 at 162.130 kb on - strand, within GFF4862 at 162.232 kb on + strand, within GFF4862 at 162.280 kb on - strand, within GFF4862 at 162.336 kb on - strand, within GFF4862 at 162.336 kb on - strand, within GFF4862 at 162.336 kb on - strand, within GFF4862 at 162.405 kb on + strand, within GFF4862 at 162.414 kb on + strand, within GFF4862 at 162.542 kb on + strand, within GFF4862 at 162.542 kb on + strand, within GFF4862 at 162.732 kb on - strand at 162.866 kb on + strand, within GFF4863 at 163.115 kb on - strand, within GFF4863 at 163.115 kb on - strand, within GFF4863 at 163.131 kb on + strand, within GFF4863 at 163.132 kb on - strand, within GFF4863 at 163.190 kb on + strand, within GFF4863 at 163.190 kb on + strand, within GFF4863 at 163.191 kb on - strand, within GFF4863 at 163.191 kb on - strand, within GFF4863 at 163.191 kb on - strand, within GFF4863 at 163.191 kb on - strand, within GFF4863 at 163.191 kb on - strand, within GFF4863 at 163.314 kb on + strand, within GFF4863 at 163.356 kb on - strand, within GFF4863 at 163.356 kb on - strand, within GFF4863 at 163.492 kb on - strand, within GFF4863
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 161,138 - GFF4860 0.76 +0.4 161,247 + GFF4860 0.84 -0.5 161,424 - +1.4 161,436 - -0.2 161,672 + GFF4861 0.45 -1.0 161,673 - GFF4861 0.45 +0.2 162,044 + GFF4862 0.12 -0.4 162,085 + GFF4862 0.17 -0.7 162,125 + GFF4862 0.23 -0.1 162,126 - GFF4862 0.23 -0.0 162,130 - GFF4862 0.24 +0.2 162,130 - GFF4862 0.24 +0.1 162,232 + GFF4862 0.38 -0.5 162,280 - GFF4862 0.45 -0.2 162,336 - GFF4862 0.53 -0.2 162,336 - GFF4862 0.53 -0.3 162,336 - GFF4862 0.53 +0.7 162,405 + GFF4862 0.62 -0.1 162,414 + GFF4862 0.64 -0.5 162,542 + GFF4862 0.82 +0.0 162,542 + GFF4862 0.82 -0.8 162,732 - -0.1 162,866 + GFF4863 0.12 +0.7 163,115 - GFF4863 0.35 -0.0 163,115 - GFF4863 0.35 +1.6 163,131 + GFF4863 0.36 -0.1 163,132 - GFF4863 0.37 +1.2 163,190 + GFF4863 0.42 +1.6 163,190 + GFF4863 0.42 -0.5 163,191 - GFF4863 0.42 +0.6 163,191 - GFF4863 0.42 -0.2 163,191 - GFF4863 0.42 +1.2 163,191 - GFF4863 0.42 -0.0 163,191 - GFF4863 0.42 -0.5 163,314 + GFF4863 0.53 +0.5 163,356 - GFF4863 0.57 -1.0 163,356 - GFF4863 0.57 +0.5 163,492 - GFF4863 0.69 +0.1
Or see this region's nucleotide sequence