Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4771 and GFF4772 are separated by 530 nucleotides GFF4772 and GFF4773 are separated by 207 nucleotides GFF4773 and GFF4774 are separated by 55 nucleotides GFF4774 and GFF4775 overlap by 4 nucleotides
GFF4771 - Serine/threonine specific protein phosphatase 2 (EC 3.1.3.16), at 78,554 to 79,210
GFF4771
GFF4772 - hypothetical protein, at 79,741 to 79,854
GFF4772
GFF4773 - DNA mismatch repair protein MutS, at 80,062 to 82,629
GFF4773
GFF4774 - FIG074102: hypothetical protein, at 82,685 to 83,065
GFF4774
GFF4775 - Possible membrane transport protein, at 83,062 to 84,270
GFF4775
Position (kb) 80
81
82
83 Strain fitness (log2 ratio) -1
0
1 at 79.227 kb on + strand at 79.274 kb on + strand at 79.275 kb on - strand at 79.577 kb on + strand at 79.697 kb on + strand at 79.716 kb on + strand at 79.788 kb on + strand, within GFF4772 at 79.906 kb on + strand at 79.907 kb on - strand at 80.263 kb on - strand at 80.341 kb on - strand, within GFF4773 at 80.341 kb on - strand, within GFF4773 at 80.391 kb on + strand, within GFF4773 at 80.488 kb on - strand, within GFF4773 at 80.494 kb on + strand, within GFF4773 at 80.501 kb on - strand, within GFF4773 at 80.606 kb on - strand, within GFF4773 at 80.634 kb on - strand, within GFF4773 at 80.768 kb on + strand, within GFF4773 at 80.794 kb on + strand, within GFF4773 at 80.798 kb on + strand, within GFF4773 at 80.798 kb on + strand, within GFF4773 at 80.872 kb on - strand, within GFF4773 at 80.958 kb on + strand, within GFF4773 at 81.029 kb on + strand, within GFF4773 at 81.029 kb on + strand, within GFF4773 at 81.467 kb on - strand, within GFF4773 at 81.488 kb on - strand, within GFF4773 at 81.533 kb on - strand, within GFF4773 at 81.574 kb on - strand, within GFF4773 at 81.586 kb on - strand, within GFF4773 at 81.648 kb on - strand, within GFF4773 at 81.650 kb on - strand, within GFF4773 at 81.693 kb on - strand, within GFF4773 at 81.767 kb on - strand, within GFF4773 at 81.784 kb on - strand, within GFF4773 at 81.934 kb on + strand, within GFF4773 at 81.935 kb on - strand, within GFF4773 at 81.971 kb on - strand, within GFF4773 at 82.113 kb on + strand, within GFF4773 at 82.171 kb on + strand, within GFF4773 at 82.171 kb on + strand, within GFF4773 at 82.225 kb on - strand, within GFF4773 at 82.253 kb on - strand, within GFF4773 at 82.330 kb on - strand, within GFF4773 at 83.008 kb on + strand, within GFF4774 at 83.055 kb on + strand at 83.120 kb on + strand at 83.121 kb on - strand at 83.157 kb on + strand at 83.158 kb on - strand at 83.305 kb on - strand, within GFF4775 at 83.305 kb on - strand, within GFF4775 at 83.416 kb on + strand, within GFF4775 at 83.426 kb on - strand, within GFF4775 at 83.589 kb on - strand, within GFF4775 at 83.623 kb on - strand, within GFF4775
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 79,227 + -0.5 79,274 + -0.1 79,275 - +0.6 79,577 + -1.0 79,697 + +0.4 79,716 + +1.5 79,788 + GFF4772 0.41 +0.7 79,906 + -0.3 79,907 - -1.2 80,263 - +0.2 80,341 - GFF4773 0.11 +0.9 80,341 - GFF4773 0.11 +0.3 80,391 + GFF4773 0.13 +0.6 80,488 - GFF4773 0.17 +1.4 80,494 + GFF4773 0.17 -0.6 80,501 - GFF4773 0.17 +0.2 80,606 - GFF4773 0.21 -1.1 80,634 - GFF4773 0.22 -0.2 80,768 + GFF4773 0.27 +1.0 80,794 + GFF4773 0.29 +0.5 80,798 + GFF4773 0.29 +0.0 80,798 + GFF4773 0.29 -0.1 80,872 - GFF4773 0.32 +0.0 80,958 + GFF4773 0.35 -1.6 81,029 + GFF4773 0.38 -0.2 81,029 + GFF4773 0.38 +0.8 81,467 - GFF4773 0.55 +0.7 81,488 - GFF4773 0.56 +0.3 81,533 - GFF4773 0.57 +0.2 81,574 - GFF4773 0.59 -0.7 81,586 - GFF4773 0.59 +0.3 81,648 - GFF4773 0.62 -0.3 81,650 - GFF4773 0.62 +0.3 81,693 - GFF4773 0.64 +0.2 81,767 - GFF4773 0.66 +1.1 81,784 - GFF4773 0.67 +1.0 81,934 + GFF4773 0.73 -0.8 81,935 - GFF4773 0.73 -0.3 81,971 - GFF4773 0.74 +0.7 82,113 + GFF4773 0.80 -0.4 82,171 + GFF4773 0.82 +0.2 82,171 + GFF4773 0.82 -0.0 82,225 - GFF4773 0.84 +0.4 82,253 - GFF4773 0.85 +1.2 82,330 - GFF4773 0.88 -0.0 83,008 + GFF4774 0.85 -1.4 83,055 + -0.6 83,120 + -1.6 83,121 - -0.8 83,157 + -0.3 83,158 - -0.3 83,305 - GFF4775 0.20 -0.4 83,305 - GFF4775 0.20 -0.2 83,416 + GFF4775 0.29 +0.7 83,426 - GFF4775 0.30 -0.4 83,589 - GFF4775 0.44 +0.6 83,623 - GFF4775 0.46 -0.4
Or see this region's nucleotide sequence