Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4744

Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4742 and GFF4743 are separated by 17 nucleotidesGFF4743 and GFF4744 are separated by 53 nucleotidesGFF4744 and GFF4745 are separated by 10 nucleotidesGFF4745 and GFF4746 are separated by 26 nucleotidesGFF4746 and GFF4747 are separated by 79 nucleotides GFF4742 - Type III secretion transcriptional activator HilA, at 49,639 to 51,300 GFF4742 GFF4743 - Invasion protein IagB precursor, at 51,318 to 51,800 GFF4743 GFF4744 - Type III secretion injected virulence protein (YopH,tyrosine phosphatase of FAK and p130cas, prevents phagocytosis), at 51,854 to 53,461 GFF4744 GFF4745 - secretion chaparone, at 53,472 to 53,864 GFF4745 GFF4746 - doubtful CDS found within S. typhi pathogenicity island, at 53,891 to 54,115 GFF4746 GFF4747 - Acyl carrier protein, at 54,195 to 54,443 GFF4747 Position (kb) 51 52 53 54Strain fitness (log2 ratio) -2 -1 0 1at 51.902 kb on + strandat 51.932 kb on - strandat 51.976 kb on + strandat 51.994 kb on + strandat 52.199 kb on - strand, within GFF4744at 52.228 kb on + strand, within GFF4744at 52.228 kb on + strand, within GFF4744at 52.228 kb on + strand, within GFF4744at 52.233 kb on + strand, within GFF4744at 52.328 kb on + strand, within GFF4744at 52.361 kb on + strand, within GFF4744at 52.484 kb on - strand, within GFF4744at 52.535 kb on - strand, within GFF4744at 52.793 kb on - strand, within GFF4744at 52.793 kb on - strand, within GFF4744at 53.709 kb on - strand, within GFF4745at 54.377 kb on + strand, within GFF4747at 54.378 kb on - strand, within GFF4747

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI
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51,902 + -0.3
51,932 - -0.6
51,976 + +0.4
51,994 + -0.8
52,199 - GFF4744 0.21 +0.2
52,228 + GFF4744 0.23 -0.6
52,228 + GFF4744 0.23 -0.1
52,228 + GFF4744 0.23 -0.6
52,233 + GFF4744 0.24 -0.4
52,328 + GFF4744 0.29 -0.3
52,361 + GFF4744 0.32 -0.6
52,484 - GFF4744 0.39 +0.6
52,535 - GFF4744 0.42 -0.2
52,793 - GFF4744 0.58 -2.0
52,793 - GFF4744 0.58 +0.3
53,709 - GFF4745 0.60 -0.2
54,377 + GFF4747 0.73 -0.7
54,378 - GFF4747 0.73 -0.7

Or see this region's nucleotide sequence