Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4696

Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4695 and GFF4696 are separated by 253 nucleotidesGFF4696 and GFF4697 overlap by 4 nucleotidesGFF4697 and GFF4698 are separated by 11 nucleotides GFF4695 - Membrane-bound lytic murein transglycosylase B precursor (EC 3.2.1.-), at 6,755 to 7,834 GFF4695 GFF4696 - PTS system, glucitol/sorbitol-specific IIC component (EC 2.7.1.69), at 8,088 to 8,651 GFF4696 GFF4697 - PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components (EC 2.7.1.69), at 8,648 to 9,619 GFF4697 GFF4698 - PTS system, glucitol/sorbitol-specific IIA component (EC 2.7.1.69), at 9,631 to 9,993 GFF4698 Position (kb) 8 9Strain fitness (log2 ratio) -2 -1 0 1 2at 7.162 kb on + strand, within GFF4695at 7.163 kb on - strand, within GFF4695at 7.163 kb on - strand, within GFF4695at 7.292 kb on - strand, within GFF4695at 7.339 kb on + strand, within GFF4695at 7.339 kb on + strand, within GFF4695at 7.340 kb on - strand, within GFF4695at 7.340 kb on - strand, within GFF4695at 7.340 kb on - strand, within GFF4695at 7.399 kb on - strand, within GFF4695at 7.401 kb on - strand, within GFF4695at 7.401 kb on - strand, within GFF4695at 7.566 kb on + strand, within GFF4695at 7.567 kb on - strand, within GFF4695at 7.567 kb on - strand, within GFF4695at 7.579 kb on + strand, within GFF4695at 7.627 kb on + strand, within GFF4695at 7.627 kb on + strand, within GFF4695at 7.640 kb on - strand, within GFF4695at 7.699 kb on + strand, within GFF4695at 7.876 kb on - strandat 7.915 kb on + strandat 8.102 kb on + strandat 8.174 kb on + strand, within GFF4696at 8.289 kb on + strand, within GFF4696at 8.290 kb on - strand, within GFF4696at 8.299 kb on + strand, within GFF4696at 8.383 kb on - strand, within GFF4696at 8.386 kb on - strand, within GFF4696at 8.400 kb on + strand, within GFF4696at 8.435 kb on + strand, within GFF4696at 8.435 kb on + strand, within GFF4696at 8.524 kb on - strand, within GFF4696at 8.534 kb on - strand, within GFF4696at 8.534 kb on - strand, within GFF4696at 8.711 kb on - strandat 8.732 kb on + strandat 8.732 kb on + strandat 8.734 kb on + strandat 8.734 kb on + strandat 8.735 kb on - strandat 8.735 kb on - strandat 8.781 kb on + strand, within GFF4697at 8.951 kb on + strand, within GFF4697at 8.954 kb on - strand, within GFF4697at 8.970 kb on - strand, within GFF4697at 8.998 kb on + strand, within GFF4697at 9.142 kb on - strand, within GFF4697at 9.233 kb on - strand, within GFF4697at 9.233 kb on - strand, within GFF4697at 9.579 kb on + strandat 9.580 kb on - strandat 9.608 kb on - strandat 9.618 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI
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7,162 + GFF4695 0.38 -0.1
7,163 - GFF4695 0.38 -0.1
7,163 - GFF4695 0.38 +0.2
7,292 - GFF4695 0.50 +0.9
7,339 + GFF4695 0.54 +1.5
7,339 + GFF4695 0.54 +0.1
7,340 - GFF4695 0.54 +0.5
7,340 - GFF4695 0.54 +0.1
7,340 - GFF4695 0.54 +0.7
7,399 - GFF4695 0.60 -0.2
7,401 - GFF4695 0.60 +1.4
7,401 - GFF4695 0.60 -0.5
7,566 + GFF4695 0.75 -0.4
7,567 - GFF4695 0.75 -0.3
7,567 - GFF4695 0.75 -0.1
7,579 + GFF4695 0.76 +0.7
7,627 + GFF4695 0.81 -2.1
7,627 + GFF4695 0.81 -0.5
7,640 - GFF4695 0.82 +0.3
7,699 + GFF4695 0.87 +0.2
7,876 - +0.4
7,915 + -2.3
8,102 + -0.9
8,174 + GFF4696 0.15 +0.4
8,289 + GFF4696 0.36 +2.0
8,290 - GFF4696 0.36 -1.9
8,299 + GFF4696 0.37 -0.7
8,383 - GFF4696 0.52 +0.9
8,386 - GFF4696 0.53 -0.3
8,400 + GFF4696 0.55 +0.2
8,435 + GFF4696 0.62 -0.2
8,435 + GFF4696 0.62 +0.7
8,524 - GFF4696 0.77 +0.5
8,534 - GFF4696 0.79 +0.6
8,534 - GFF4696 0.79 +0.0
8,711 - +1.7
8,732 + -0.4
8,732 + -0.5
8,734 + -2.1
8,734 + +0.3
8,735 - +0.1
8,735 - +1.7
8,781 + GFF4697 0.14 -1.1
8,951 + GFF4697 0.31 +0.6
8,954 - GFF4697 0.31 +0.2
8,970 - GFF4697 0.33 +0.4
8,998 + GFF4697 0.36 +0.3
9,142 - GFF4697 0.51 -0.2
9,233 - GFF4697 0.60 -0.6
9,233 - GFF4697 0.60 -0.2
9,579 + -0.3
9,580 - -0.1
9,608 - -0.3
9,618 - -0.1

Or see this region's nucleotide sequence