Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4542 and GFF4543 are separated by 301 nucleotides GFF4543 and GFF4544 are separated by 99 nucleotides GFF4544 and GFF4545 are separated by 108 nucleotides
GFF4542 - LysR-family transcriptional regulator YbeF, at 326,247 to 327,200
GFF4542
GFF4543 - Octanoate-[acyl-carrier-protein]-protein-N-octan oyltransferase, at 327,502 to 328,143
GFF4543
GFF4544 - Proposed lipoate regulatory protein YbeD, at 328,243 to 328,506
GFF4544
GFF4545 - D-alanyl-D-alanine carboxypeptidase (EC 3.4.16.4), at 328,615 to 329,826
GFF4545
Position (kb) 327
328
329 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 326.680 kb on + strand, within GFF4542 at 326.680 kb on + strand, within GFF4542 at 326.680 kb on + strand, within GFF4542 at 326.685 kb on - strand, within GFF4542 at 326.720 kb on + strand, within GFF4542 at 326.858 kb on + strand, within GFF4542 at 326.863 kb on + strand, within GFF4542 at 326.864 kb on - strand, within GFF4542 at 326.917 kb on + strand, within GFF4542 at 326.917 kb on + strand, within GFF4542 at 326.938 kb on + strand, within GFF4542 at 326.938 kb on + strand, within GFF4542 at 327.000 kb on + strand, within GFF4542 at 327.072 kb on - strand, within GFF4542 at 327.082 kb on - strand, within GFF4542 at 328.154 kb on - strand at 328.183 kb on - strand at 328.183 kb on - strand at 328.185 kb on - strand at 328.244 kb on + strand at 328.245 kb on - strand at 328.245 kb on - strand at 328.522 kb on - strand at 328.574 kb on - strand at 328.577 kb on - strand at 328.577 kb on - strand at 328.578 kb on + strand at 328.579 kb on - strand at 328.802 kb on - strand, within GFF4545 at 328.805 kb on + strand, within GFF4545 at 328.805 kb on + strand, within GFF4545 at 329.005 kb on - strand, within GFF4545 at 329.007 kb on - strand, within GFF4545
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 326,680 + GFF4542 0.45 +0.9 326,680 + GFF4542 0.45 -3.2 326,680 + GFF4542 0.45 -1.3 326,685 - GFF4542 0.46 -0.4 326,720 + GFF4542 0.50 -0.3 326,858 + GFF4542 0.64 +0.4 326,863 + GFF4542 0.65 +0.4 326,864 - GFF4542 0.65 +0.3 326,917 + GFF4542 0.70 +0.5 326,917 + GFF4542 0.70 -0.4 326,938 + GFF4542 0.72 -0.0 326,938 + GFF4542 0.72 +0.2 327,000 + GFF4542 0.79 -0.4 327,072 - GFF4542 0.86 -0.2 327,082 - GFF4542 0.88 -1.4 328,154 - +0.5 328,183 - +1.1 328,183 - -0.4 328,185 - +0.0 328,244 + +0.4 328,245 - -1.2 328,245 - -0.4 328,522 - +0.6 328,574 - +0.9 328,577 - +0.4 328,577 - -1.0 328,578 + +1.4 328,579 - +0.7 328,802 - GFF4545 0.15 +0.1 328,805 + GFF4545 0.16 +1.6 328,805 + GFF4545 0.16 +0.2 329,005 - GFF4545 0.32 +1.0 329,007 - GFF4545 0.32 +0.4
Or see this region's nucleotide sequence