Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF452 and GFF453 are separated by 844 nucleotides GFF453 and GFF454 are separated by 173 nucleotides GFF454 and GFF455 are separated by 288 nucleotides
GFF452 - FIG032766: hypothetical protein, at 174,603 to 174,869
GFF452
GFF453 - FIG00923183: possible membrane protein YebY, at 175,714 to 176,253
GFF453
GFF454 - Cold shock protein CspA, at 176,427 to 176,639
GFF454
GFF455 - FIG00638451: hypothetical protein, at 176,928 to 177,218
GFF455
Position (kb) 175
176
177 Strain fitness (log2 ratio) -1
0
1 at 175.118 kb on - strand at 175.119 kb on + strand at 175.120 kb on - strand at 175.227 kb on + strand at 175.253 kb on - strand at 175.328 kb on - strand at 175.328 kb on - strand at 175.341 kb on - strand at 175.398 kb on - strand at 175.418 kb on + strand at 175.540 kb on + strand at 175.799 kb on - strand, within GFF453 at 176.031 kb on + strand, within GFF453 at 176.053 kb on + strand, within GFF453 at 176.233 kb on + strand at 176.253 kb on + strand at 176.286 kb on + strand at 176.336 kb on - strand at 176.559 kb on - strand, within GFF454 at 176.577 kb on - strand, within GFF454 at 176.580 kb on - strand, within GFF454 at 176.614 kb on - strand, within GFF454 at 176.640 kb on - strand at 176.640 kb on - strand at 176.645 kb on - strand at 176.648 kb on - strand at 176.768 kb on - strand at 176.848 kb on + strand at 176.894 kb on - strand at 176.949 kb on + strand at 176.950 kb on - strand at 177.097 kb on - strand, within GFF455 at 177.209 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 175,118 - -0.2 175,119 + +0.0 175,120 - +0.1 175,227 + -0.8 175,253 - -1.0 175,328 - -0.6 175,328 - -0.9 175,341 - -1.4 175,398 - +0.3 175,418 + -0.7 175,540 + +0.6 175,799 - GFF453 0.16 -0.1 176,031 + GFF453 0.59 -0.9 176,053 + GFF453 0.63 -1.3 176,233 + -0.1 176,253 + +0.5 176,286 + -1.2 176,336 - -0.9 176,559 - GFF454 0.62 +0.3 176,577 - GFF454 0.70 -0.1 176,580 - GFF454 0.72 +0.4 176,614 - GFF454 0.88 +0.1 176,640 - -0.4 176,640 - -0.5 176,645 - -1.2 176,648 - -0.1 176,768 - -0.3 176,848 + +0.1 176,894 - -0.9 176,949 + -0.0 176,950 - -0.2 177,097 - GFF455 0.58 +0.2 177,209 + +1.3
Or see this region's nucleotide sequence