Experiment: LB_plus_SM_buffer with 8C2_phage 15 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4525 and GFF4526 overlap by 26 nucleotides GFF4526 and GFF4527 are separated by 3 nucleotides GFF4527 and GFF4528 are separated by 9 nucleotides GFF4528 and GFF4529 overlap by 4 nucleotides
GFF4525 - '2-(5''-triphosphoribosyl)-3'-dephosphocoenzyme- A synthase (EC 2.7.8.25)' transl_table=11, at 312,620 to 313,513
GFF4525
GFF4526 - Apo-citrate lyase phosphoribosyl-dephospho-CoA transferase (EC 2.7.7.61), at 313,488 to 314,039
GFF4526
GFF4527 - Citrate lyase alpha chain (EC 4.1.3.6), at 314,043 to 315,572
GFF4527
GFF4528 - Citrate lyase beta chain (EC 4.1.3.6), at 315,582 to 316,490
GFF4528
GFF4529 - Citrate lyase gamma chain, acyl carrier protein (EC 4.1.3.6), at 316,487 to 316,783
GFF4529
Position (kb) 314
315
316 Strain fitness (log2 ratio) -1
0
1 at 313.118 kb on + strand, within GFF4525 at 313.118 kb on + strand, within GFF4525 at 313.118 kb on + strand, within GFF4525 at 313.119 kb on - strand, within GFF4525 at 313.644 kb on + strand, within GFF4526 at 313.816 kb on + strand, within GFF4526 at 313.911 kb on - strand, within GFF4526 at 314.044 kb on + strand at 314.507 kb on + strand, within GFF4527 at 314.776 kb on + strand, within GFF4527 at 315.041 kb on - strand, within GFF4527 at 315.117 kb on + strand, within GFF4527 at 315.117 kb on + strand, within GFF4527 at 315.218 kb on - strand, within GFF4527 at 315.806 kb on + strand, within GFF4528 at 315.806 kb on + strand, within GFF4528 at 315.807 kb on - strand, within GFF4528 at 315.807 kb on - strand, within GFF4528 at 315.992 kb on - strand, within GFF4528 at 315.992 kb on - strand, within GFF4528 at 316.315 kb on - strand, within GFF4528 at 316.395 kb on - strand, within GFF4528 at 316.442 kb on + strand at 316.443 kb on - strand at 316.465 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with 8C2_phage 15 MOI remove 313,118 + GFF4525 0.56 +1.1 313,118 + GFF4525 0.56 -0.2 313,118 + GFF4525 0.56 +0.1 313,119 - GFF4525 0.56 -0.1 313,644 + GFF4526 0.28 +0.4 313,816 + GFF4526 0.59 +0.6 313,911 - GFF4526 0.77 +0.7 314,044 + +0.7 314,507 + GFF4527 0.30 +0.2 314,776 + GFF4527 0.48 +1.7 315,041 - GFF4527 0.65 -1.5 315,117 + GFF4527 0.70 +0.5 315,117 + GFF4527 0.70 +0.5 315,218 - GFF4527 0.77 -0.6 315,806 + GFF4528 0.25 -0.0 315,806 + GFF4528 0.25 -0.2 315,807 - GFF4528 0.25 -0.5 315,807 - GFF4528 0.25 -0.2 315,992 - GFF4528 0.45 -1.6 315,992 - GFF4528 0.45 -1.1 316,315 - GFF4528 0.81 +0.0 316,395 - GFF4528 0.89 +0.1 316,442 + -0.3 316,443 - -0.2 316,465 - -1.2
Or see this region's nucleotide sequence